{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T10:02:55Z","timestamp":1789725775696,"version":"4.0.1"},"reference-count":27,"publisher":"BMJ","issue":"10","funder":[{"name":"Program Management Unit for Competitiveness","award":["C01F650206"],"award-info":[{"award-number":["C01F650206"]}]},{"name":"Program Management Unit for Competitiveness","award":["C01F670186"],"award-info":[{"award-number":["C01F670186"]}]},{"name":"Program Management Unit for Competitiveness","award":["C01F690069"],"award-info":[{"award-number":["C01F690069"]}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2026,7,5]]},"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To address the absence of nationally accessible proficiency testing (PT) for biospecimens in Thailand, we developed and evaluated a pilot PT programme consisting of four schemes covering DNA extraction, quantification, purity and integrity assessment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The pilot PT programme comprised two\n                      <jats:italic>processing<\/jats:italic>\n                      schemes: DNA extraction from whole blood (P-DNABLD) and frozen tissue (P-DNAFRT) and two\n                      <jats:italic>testing<\/jats:italic>\n                      schemes: DNA quantification and purity (T-DNAQ) and DNA integrity (T-DNAI). All schemes were conducted in accordance with ISO\/IEC 17043:2023 and ISO 13528:2022. PT items were assessed for homogeneity and stability, and participant performance was evaluated based on consensus values using\n                      <jats:italic>z<\/jats:italic>\n                      - or\n                      <jats:italic>z<\/jats:italic>\n                      \u2032-scores.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All PT items satisfied the ISO 13528:2022 requirements for homogeneity and stability after accounting for instability-related uncertainty in T-DNAQ. 30 laboratories participated nationwide in at least one scheme (P-DNABLD, n=28; P-DNAFRT, n=17; T-DNAQ, n=29; T-DNAI, n=13). All PT items were received within 3\u2009days of dispatch, with \u226590% arriving within the acceptable temperature range. DNA extraction yield-related measurands demonstrated substantial interlaboratory variability (CV 78.2\u201295.3%), whereas purity and integrity measurands were more consistent (CV 1.3\u201211.2%). In T-DNAQ, A\n                      <jats:sub>260<\/jats:sub>\n                      \/A\n                      <jats:sub>230<\/jats:sub>\n                      showed the greatest interlaboratory variability (CV 8.2\u201216.4%); within-platform variability was low in T-DNAI. Across all schemes, \u226575% of participants achieved at least Satisfactory performance (|z or z\u2032| \u2264 2.0). Direct costs were approximately 12% of those of comparable international PT schemes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This pilot demonstrates the feasibility and accessibility of a nationally coordinated PT programme for biospecimens and supports the establishment of a sustainable PT framework to harmonise biospecimen quality practices.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jcp-2026-210762","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T17:20:27Z","timestamp":1785172827000},"page":"702-712","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Developing a national proficiency testing programme for biospecimens: the Thailand Biobank Consortium experience"],"prefix":"10.1136","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3689-8184","authenticated-orcid":false,"given":"Piyavat","family":"Apisampinvong","sequence":"first","affiliation":[{"name":"Center of Multidisciplinary Technology for Advanced Medicine (CMUTEAM), Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand"},{"name":"Integrated Research Infrastructure Center (iRIC), Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0235-7747","authenticated-orcid":false,"given":"Chawanit","family":"Wongkasa","sequence":"additional","affiliation":[{"name":"Ramathibodi Comprehensive Cancer Center, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1416-8277","authenticated-orcid":false,"given":"Thanastha","family":"Thanomchard","sequence":"additional","affiliation":[{"name":"Ramathibodi Comprehensive Cancer Center, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natthanich","family":"Denchairat","sequence":"additional","affiliation":[{"name":"Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natthaorn","family":"Luepongpatthana","sequence":"additional","affiliation":[{"name":"Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratikorn","family":"Kamngoen","sequence":"additional","affiliation":[{"name":"Center of Multidisciplinary Technology for Advanced Medicine (CMUTEAM), Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand"},{"name":"Integrated Research Infrastructure Center (iRIC), Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rassanee","family":"Bissanum","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand"},{"name":"Translational Medicine Research Center, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pasarat","family":"Khongkow","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand"},{"name":"Translational Medicine Research Center, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunchit","family":"Judprasong","sequence":"additional","affiliation":[{"name":"Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhitiwass","family":"Suvagandha","sequence":"additional","affiliation":[{"name":"Division of Proficiency Testing, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3595-8003","authenticated-orcid":false,"given":"Arnat","family":"Pasena","sequence":"additional","affiliation":[{"name":"Center of Multidisciplinary Technology for Advanced Medicine (CMUTEAM), Faculty of Medicine, 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Integrating the DNA integrity number (DIN) to assess genomic DNA (gDNA) quality control using the agilent 2200 TapeStation system. In: Agilent technologies application note. Agilent Technologies, 2016."},{"key":"2026091802100904000_79.10.702.25","unstructured":"Brookman B , Mann I , eds. Eurachem guide: selection, use and interpretation of Proficiency Testing (PT) schemes, 3rd ed. 2021. Available: www.eurachem.org"},{"key":"2026091802100904000_79.10.702.26","unstructured":"International Organization for Standardization . ISO\/IEC 13528:2022 statistical methods for use in proficiency testing by interlaboratory comparison. 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