{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T17:14:02Z","timestamp":1763745242549,"version":"3.45.0"},"reference-count":8,"publisher":"BMJ","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1964,1]]},"DOI":"10.1136\/jcp.17.1.96","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"96-97","source":"Crossref","is-referenced-by-count":1,"title":["A micromethod for estimating osmotic fragility of erythrocytes"],"prefix":"10.1136","volume":"17","author":[{"given":"S. T. G.","family":"Butterworth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1964,1,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1002\/path.1700460213","volume":"46","author":"Creed, E.ff","year":"1938","unstructured":"Creed, E. ff. (1938). J. Path. Bact., 46, 331.","journal-title":"J. Path. Bact"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1290","DOI":"10.1182\/blood.V3.11.1290.1290","volume":"3","author":"Suess, J.; Limentani, D.; Dameshek, W.; Dolloff, M.J.","year":"1948","unstructured":"Suess, J., Limentani, D., Dameshek, W., and Dolloff, M. J. (1948). Blood, 3, 1290.","journal-title":"Blood"},{"key":"ref_3","article-title":"Practical Haematology","author":"Dacie, J.V.","year":"1956","unstructured":"Dacie, J. V. (1956). Practical Haematology, Churchill, London."},{"key":"ref_4","article-title":"Pakistan Soya and jack beans are commonly used for the preparation of urease in the estimation of blood urea but Damodaran and Sivaramakrishnan","volume-title":"demonstrated that the seeds of water melon (Citrullus vulgaris) had a high urease content. The raw water melon seeds without any treatment have been used as a source of urease in this work. The potency of urease was high in all healthy seeds as compared with Merck urease. Both these preparations of urease gave results with standard urea solutions and blood, which were within the range of error as advocated by King and Wootton","author":"College, From Dacca Medical","year":"1937","unstructured":"From Dacca Medical College, Pakistan Soya and jack beans are commonly used for the preparation of urease in the estimation of blood urea but Damodaran and Sivaramakrishnan (1937) demonstrated that the seeds of water melon (Citrullus vulgaris) had a high urease content. The raw water melon seeds without any treatment have been used as a source of urease in this work. The potency of urease was high in all healthy seeds as compared with Merck urease. Both these preparations of urease gave results with standard urea solutions and blood, which were within the range of error as advocated by King and Wootton (1956)."},{"key":"ref_5","unstructured":"The presence of urease in the soya bean (Soja) was demonstrated as early as 1909.","article-title":"The presence of urease in the soya bean (Soja) was"},{"key":"ref_6","article-title":"found the high urease potency in the family Papilonaceae including jack bean and horse gram (Dolichos biflorus) but the activity of the latter deteriorated rapidly on storage","author":"Annett","year":"1914","unstructured":"Annett (1914) found the high urease potency in the family Papilonaceae including jack bean and horse gram (Dolichos biflorus) but the activity of the latter deteriorated rapidly on storage. Klein (1933) demonstrated the presence of urease within the family Cucurbitaceae. Damodaran and Sivaramakrishnan (1937) concluded that the urease from the seeds of the water melon are superior to those from the soya and jack beans as it is free from the errors that pertain to the latter ones.","journal-title":"Klein; demonstrated the presence of urease within the family Cucurbitaceae. Damodaran and Sivaramakrishnan"},{"key":"ref_7","article-title":"The seeds which did not look healthy were discarded. The healthy seeds were washed in tap water to remove the sticky juice of the fruit from the surface and then dried in the air in the shade. They were then kept at room temperature. For use, seeds were cut in the longitudinal axis with a fine knife along the borders. Three preparations were made: I Half of the pulp of a seed chopped into fine particles; 2 a number of pulps cut into fine particles and preserved at room temperature; 3 a number of pulps cut into fine particles and preserved in the refrigerator. UREASE (MERCK, E) This is manufactured from jack bean meal. The reagents and method of urea estimation were the same as described by King and Wootton","volume-title":"except for the Nessler reagent which was made up by the method of Bock and Benedict","author":"VULGARIS) SEEDS","year":"1956","unstructured":"VULGARIS) SEEDS. The seeds which did not look healthy were discarded. The healthy seeds were washed in tap water to remove the sticky juice of the fruit from the surface and then dried in the air in the shade. They were then kept at room temperature. For use, seeds were cut in the longitudinal axis with a fine knife along the borders. Three preparations were made: I Half of the pulp of a seed chopped into fine particles; 2 a number of pulps cut into fine particles and preserved at room temperature; 3 a number of pulps cut into fine particles and preserved in the refrigerator. UREASE (MERCK, E) This is manufactured from jack bean meal. The reagents and method of urea estimation were the same as described by King and Wootton (1956) except for the Nessler reagent which was made up by the method of Bock and Benedict (Harrison, 1957)."},{"key":"ref_8","unstructured":"The common mode of preparation (Van Slyke and Cullen, 1914) of urease requires a large quantity of acetone.","article-title":"The common mode of preparation (Van Slyke and Cullen, 1914) of urease requires a large quantity of acetone"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.17.1.96","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T17:05:57Z","timestamp":1763744757000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.17.1.96"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1964,1]]},"references-count":8,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1964,1,1]]},"published-print":{"date-parts":[[1964,1]]}},"alternative-id":["10.1136\/jcp.17.1.96"],"URL":"https:\/\/doi.org\/10.1136\/jcp.17.1.96","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1964,1]]}}}