{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T05:17:30Z","timestamp":1765603050546,"version":"3.48.0"},"reference-count":13,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2004,6]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    In sheep, susceptibility to scrapie, which is similar to human prion diseases such as Kuru and variant Creutzfeldt-Jakob disease (vCJD), is determined by prion protein (PrP) gene (Prnp) polymorphisms. Sheep with genotype ARQ\/ARQ, denoting polymorphisms at codons 136, 154, and 171, are susceptible, whereas those with genotypes ARR\/ARQ and ARR\/ARR are resistant, indicating dominance of ARR over the ARQ allele.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aims:<\/jats:bold>\n                    Based on familial CJD E200K, 129V, where preferential use of the 200E allele in EK heterozygous individuals confers resistance, heterozygous ARR\/ARQ sheep were used to test the hypothesis that resistance is caused by preferential use of the ARR allele.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    After assessment of equivalent PrP expression across genotypes, allele use was analysed by sequencing reverse transcription polymerase chain reaction derived DNA clones containing the Prnp gene coding sequence.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    The ARR to ARQ ratio was 1.1 in 133 clones, representing Prnp mRNA from three ARR\/ARQ sheep, indicating equal use of both alleles.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    Dominance of the resistant associated allele in sheep scrapie involves mechanisms other than the absence of PrP derived from the disease associated ARQ allele.\n                  <\/jats:p>","DOI":"10.1136\/jcp.2003.012203","type":"journal-article","created":{"date-parts":[[2004,5,27]],"date-time":"2004-05-27T13:48:07Z","timestamp":1085665687000},"page":"647-650","source":"Crossref","is-referenced-by-count":11,"title":["Resistance to scrapie in PrP ARR\/ARQ heterozygous sheep is not caused by preferential allelic use"],"prefix":"10.1136","volume":"57","author":[{"given":"P A","family":"Caplazi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K I","family":"O\u2019Rourke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T V","family":"Baszler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2004,5,27]]},"reference":[{"key":"2025121221125816000_57.6.647.1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.7.2476"},{"key":"2025121221125816000_57.6.647.2","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1099\/0022-1317-78-4-975","volume":"78","year":"1997","journal-title":"J Gen Virol"},{"key":"2025121221125816000_57.6.647.3","first-page":"143","volume":"16","year":"2000","journal-title":"Arch Virol 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