{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T03:33:32Z","timestamp":1765596812870,"version":"3.48.0"},"reference-count":18,"publisher":"BMJ","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Proliferating and tumour cells express the glycolytic isoenzyme, pyruvate kinase type M2 (M2-PK). In tumours cells, M2-PK usually exists in dimeric form (tumour M2-PK), causing the accumulation of glycolytic phosphometabolites, which allows cells to invade areas with low oxygen and glucose concentrations.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aims:<\/jats:bold>\n                    To investigate the expression of tumour M2-PK during the metaplasia\u2013dysplasia\u2013adenocarcinoma sequence of Barrett\u2019s oesophagus, and to assess the prognostic usefulness of tumour M2-PK in oesophageal cancer.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Materials\/Methods:<\/jats:bold>\n                    One hundred and ninety cases selected from the histopathology archives as follows: 17 reflux oesophagitis, 37 Barrett\u2019s oesophagus, 21 high grade dysplasia, 112 adenocarcinomas, and three control tumours. Sections were stained immunohistochemically with antibody to tumour M2-PK.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Tumour M2-PK was expressed in all cases, and increased cytoplasmic expression was seen with progression along the metaplasia\u2013dysplasia\u2013adenocarcinoma sequence. All cases of adenocarcinoma showed 100% staining so that tumour M2-PK was not a useful prognostic marker.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    Tumour M2-PK is not a specific marker of Barrett\u2019s adenocarcinoma, but may be important as a marker of transformed and highly proliferating clones during progression along the metaplasia\u2013dysplasia\u2013adenocarcinoma sequence.\n                  <\/jats:p>","DOI":"10.1136\/jcp.2004.018150","type":"journal-article","created":{"date-parts":[[2004,10,27]],"date-time":"2004-10-27T16:18:35Z","timestamp":1098893915000},"page":"1156-1159","source":"Crossref","is-referenced-by-count":23,"title":["The metabolic marker tumour pyruvate kinase type M2 (tumour M2-PK) shows increased expression along the metaplasia\u2013dysplasia\u2013adenocarcinoma sequence in Barrett\u2019s oesophagus"],"prefix":"10.1136","volume":"57","author":[{"given":"K","family":"Koss","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R F","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Gregory","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S J","family":"Darnton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M R","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A Z","family":"Jankowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2004,10,27]]},"reference":[{"key":"2025121219313038000_57.11.1156.1","doi-asserted-by":"publisher","DOI":"10.1016\/S1052-5157(03)00005-9"},{"key":"2025121219313038000_57.11.1156.2","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1016\/S0002-9440(10)65346-1","volume":"154","year":"1999","journal-title":"Am J Pathol"},{"key":"2025121219313038000_57.11.1156.3","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)03411-5"},{"key":"2025121219313038000_57.11.1156.4","first-page":"135","volume":"152","year":"1998","journal-title":"Am J Pathol"},{"key":"2025121219313038000_57.11.1156.5","first-page":"3153","volume":"17","year":"1997","journal-title":"Anticancer Res"},{"key":"2025121219313038000_57.11.1156.6","first-page":"531","volume":"24","year":"2000","journal-title":"Cancer Detect Prev"},{"key":"2025121219313038000_57.11.1156.7","first-page":"3267","volume":"18","year":"1998","journal-title":"Anticancer Res"},{"key":"2025121219313038000_57.11.1156.8","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204792"},{"key":"2025121219313038000_57.11.1156.9","first-page":"177","volume":"424","year":"1994","journal-title":"Virchows Arch"},{"key":"2025121219313038000_57.11.1156.10","first-page":"851","volume":"23","year":"2003","journal-title":"Anticancer Res"},{"key":"2025121219313038000_57.11.1156.11","first-page":"4961","volume":"20","year":"2000","journal-title":"Anticancer Res"},{"key":"2025121219313038000_57.11.1156.12","unstructured":"Jankowski J , Wright NA, Meltzer S, et al. 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