{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:10:48Z","timestamp":1771027848905,"version":"3.50.1"},"reference-count":44,"publisher":"BMJ","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Hashimoto\u2019s thyroiditis (HT) is a risk factor for thyroid lymphoma, and clonal B\u00a0cell populations in HT support this link. The literature on B\u00a0cell clonality in HT is controversial.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aims:<\/jats:bold>\n                    To identify clonal B\u00a0cell populations in HT and to assess their usefulness in differentiating HT from mucosa associated lymphoid tissue (MALT) lymphoma and predicting future development of lymphoma.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    DNA from formalin fixed, paraffin wax embedded blocks of thyroid specimens from 10 patients with HT and two thyroid MALT lymphomas was analysed for B\u00a0cell clonality by seminested polymerase chain reaction (PCR) using FRIII\/LJH and FRIII\/VLJH primers to amplify the IgH gene VDJ region. In one case, PCR products were sequenced. Immunohistochemistry was performed by labelled streptavidin\u2013biotin technique using antibodies to: CD45, CD45RO, CD3, CD20, and cytokeratin.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    The histopathological and clinical findings were characteristic of HT. Clonal bands were seen in three and a polyclonal smear pattern was seen in seven cases. The clonal bands in HT were associated with a background smear, and could not be reproduced from other blocks from the same case or from deeper sections of the same block. The clonal bands in thyroid lymphomas were not associated with a background smear and were reproducible. None of the patients with clonal B\u00a0cells has developed malignant lymphoma during a follow up of 10\u201313 years.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    B\u00a0cell clonal bands in HT have different features from those in lymphoma (non-pure and non-reproducible) and do not predict future development of lymphoma.\n                  <\/jats:p>","DOI":"10.1136\/jcp.2004.018416","type":"journal-article","created":{"date-parts":[[2004,11,24]],"date-time":"2004-11-24T13:12:48Z","timestamp":1101301968000},"page":"1258-1263","source":"Crossref","is-referenced-by-count":63,"title":["Clonal B cell populations in a minority of patients with Hashimoto\u2019s thyroiditis"],"prefix":"10.1136","volume":"57","author":[{"given":"A","family":"Saxena","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E C","family":"Alport","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Moshynska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Kanthan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M A","family":"Boctor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2004,11,24]]},"reference":[{"key":"2025121408145063000_57.12.1258.1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)91409-F"},{"key":"2025121408145063000_57.12.1258.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(97)90101-5"},{"key":"2025121408145063000_57.12.1258.3","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1056\/NEJM198704303161803","volume":"316","year":"1987","journal-title":"N Engl J Med"},{"key":"2025121408145063000_57.12.1258.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(97)90161-1"},{"key":"2025121408145063000_57.12.1258.5","first-page":"13","volume":"135","year":"1989","journal-title":"Am J Pathol"},{"key":"2025121408145063000_57.12.1258.6","first-page":"157","volume":"119","year":"1995","journal-title":"Arch Pathol Lab Med"},{"key":"2025121408145063000_57.12.1258.7","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1182\/blood.V73.6.1635.1635","volume":"73","year":"1989","journal-title":"Blood"},{"key":"2025121408145063000_57.12.1258.8","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1093\/oxfordjournals.annonc.a059177","volume":"6","year":"1995","journal-title":"Ann Oncol"},{"key":"2025121408145063000_57.12.1258.9","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1056\/NEJM198503073121001","volume":"312","year":"1985","journal-title":"N Engl J Med"},{"key":"2025121408145063000_57.12.1258.10","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1016\/S0046-8177(88)80287-9","volume":"19","year":"1988","journal-title":"Hum Pathol"},{"key":"2025121408145063000_57.12.1258.11","doi-asserted-by":"crossref","unstructured":"Isaacson PG. 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