{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:14:35Z","timestamp":1787505275860,"version":"build-2736575974"},"reference-count":23,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Bcl-2 protooncogene alterations are involved in tumorigenesis and may have prognostic ramifications.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aims:<\/jats:bold>\n                    To investigate normal ocular structures and choroidal melanoma for: (1) Bcl-2 protein expression (semiquantitative staining values: SI, staining intensity; PP, percentage of positive cells; and IRS, immunoreactivity score) and (2) any associations between the staining values and clinicopathological features in these lesions.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Materials\/Methods:<\/jats:bold>\n                    Bcl-2 protein expression was analysed in 24 choroidal melanomas using immunoperoxidase staining methods.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Bcl-2 protein expression was seen in corneal epithelium, lens epithelium, the ciliary body, and retinal cells. In these structures, the mean (SEM) values were: 1.1 (0.1), 1.6 (0.3), 1.1 (0.1), and 2.3 (0.3), respectively, for SI; 1.6 (0.2), 1.7 (0.1), 1.7 (0.2), and 1.7 (0.2) for PP, respectively; and 1.9 (0.4), 2.7 (0.5), 1.9 (0.1), and 4.0 (0.8), respectively, for IRS. Based on Bcl-2 immunoreactivity, the lesions were divided into two groups. The first group comprised 12 tumours with Bcl-2 expression. Bcl-2 expression was significantly higher in this group compared with normal ocular structures (1.5 (0.1)\n                    <jats:italic>v<\/jats:italic>\n                    2.8 (0.2), 1.7 (0.1)\n                    <jats:italic>v<\/jats:italic>\n                    3.5 (0.1), and 2.6 (0.3)\n                    <jats:italic>v<\/jats:italic>\n                    9.3 (0.9) for mean (SEM) SI, PP, and IRS scores, respectively; p \u200a=\u200a 0.00). The second group comprised 12 tumours lacking Bcl-2 protein expression. There was no significant correlation between Bcl-2 protein expression and most of the clinicopathological features of these lesions.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    Bcl-2 protein expression is altered in choroidal melanomas.\n                  <\/jats:p>","DOI":"10.1136\/jcp.2004.023291","type":"journal-article","created":{"date-parts":[[2005,4,27]],"date-time":"2005-04-27T16:16:29Z","timestamp":1114618589000},"page":"486-489","source":"Crossref","is-referenced-by-count":15,"title":["Analysis of Bcl-2 protein expression in choroidal melanomas"],"prefix":"10.1136","volume":"58","author":[{"given":"M R","family":"Hussein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2005,4,27]]},"reference":[{"key":"2025121402443861000_58.5.486.1","first-page":"32","volume":"118","year":"2002","journal-title":"Vestn Oftalmol"},{"key":"2025121402443861000_58.5.486.2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2003.015800"},{"key":"2025121402443861000_58.5.486.3","doi-asserted-by":"publisher","DOI":"10.1097\/00006982-200408000-00030"},{"key":"2025121402443861000_58.5.486.4","doi-asserted-by":"publisher","DOI":"10.1097\/00008469-200304000-00002"},{"key":"2025121402443861000_58.5.486.5","doi-asserted-by":"publisher","DOI":"10.1002\/path.1300"},{"key":"2025121402443861000_58.5.486.6","first-page":"497","volume":"120","year":"1996","journal-title":"Arch Pathol Lab Med"},{"key":"2025121402443861000_58.5.486.7","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1136\/bjo.83.1.110","volume":"83","year":"1999","journal-title":"Br J Ophthalmol"},{"key":"2025121402443861000_58.5.486.8","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1016\/S0002-9440(10)64817-1","volume":"157","year":"2000","journal-title":"Am J Pathol"},{"key":"2025121402443861000_58.5.486.9","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-1392"},{"key":"2025121402443861000_58.5.486.10","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-200108000-00006"},{"key":"2025121402443861000_58.5.486.11","doi-asserted-by":"publisher","DOI":"10.1002\/mc.10047"},{"key":"2025121402443861000_58.5.486.12","doi-asserted-by":"crossref","unstructured":"Hussein MR, Ismael HH. Alterations of p53, Bcl-2, and hMSH2 protein expression in the normal breast, benign proliferative breast disease, in situ and infiltrating ductal breast carcinomas in upper Egypt. Cancer Biol Ther. 2004;3.[Epub ahead of print.].","DOI":"10.4161\/cbt.3.10.1136"},{"key":"2025121402443861000_58.5.486.13","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/S0002-9440(10)64744-X","volume":"156","year":"2000","journal-title":"Am J Pathol"},{"key":"2025121402443861000_58.5.486.14","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199907130-00030"},{"key":"2025121402443861000_58.5.486.15","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.16.11245"},{"key":"2025121402443861000_58.5.486.16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102195200"},{"key":"2025121402443861000_58.5.486.17","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401384"},{"key":"2025121402443861000_58.5.486.18","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.17.10.970.5240"},{"key":"2025121402443861000_58.5.486.19","doi-asserted-by":"publisher","DOI":"10.1089\/08977150151071017"},{"key":"2025121402443861000_58.5.486.20","first-page":"150","volume":"8","year":"1995","journal-title":"Mod Pathol"},{"key":"2025121402443861000_58.5.486.21","first-page":"643","volume":"146","year":"1995","journal-title":"Am J Pathol"},{"key":"2025121402443861000_58.5.486.22","doi-asserted-by":"publisher","DOI":"10.1016\/S0748-7983(96)90176-6"},{"key":"2025121402443861000_58.5.486.23","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-200202000-00016"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.2004.023291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T10:44:51Z","timestamp":1765709091000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.2004.023291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4,27]]},"references-count":23,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2005,4,27]]},"published-print":{"date-parts":[[2005,5]]}},"alternative-id":["10.1136\/jcp.2004.023291"],"URL":"https:\/\/doi.org\/10.1136\/jcp.2004.023291","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4,27]]}}}