{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T12:02:08Z","timestamp":1780920128340,"version":"3.54.1"},"reference-count":37,"publisher":"BMJ","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p>\n                    <jats:bold>Aim:<\/jats:bold>\n                    To examine eosinophil infiltration and degranulation in 50 oesophageal biopsy specimens from 30 patients (21 men, 9 women; mean 39 years) with eosinophilic oesophagitis, by haematoxylin and eosin staining and immunohistochemistry.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    Immunohistochemistry was carried out using a monoclonal antibody for human eosinophilic major basic protein (MBP). Eosinophils were counted in three high power fields (\u00d740) and degranulation, as quantified by extracellular MBP immunostaining, was scored on a scale of 1\u20134. Morphological changes (basal cell hyperplasia, elongation of papillae and dilatation of intercellular spaces) were scored on a 1\u20134 scale on sections stained with haematoxylin and eosin.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Numbers of intraepithelial eosinophils were significantly higher with MBP immunostaining than with haematoxylin and eosin staining (mean 109.6\n                    <jats:italic>v<\/jats:italic>\n                    80.6; p&lt;0.001), whereas numbers of eosinophils were considerably correlated (r\u200a=\u200a0.794). Eosinophil degranulation was higher in the distal oesophagus. Additionally, basic morphological changes were markedly associated with eosinophil infiltration. Extracellular deposition of eosinophil-MBP and eosinophil infiltration in subepithelial connective tissue, present in the biopsy specimens, were detected by immunohistochemistry.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    Numbers of eosinophils and degranulation are underestimated by haematoxylin and eosin staining. Immunohistochemistry detected up to two times more eosinophils than routine haematoxylin and eosin staining. Moreover, eosinophil-MBP immunoreactivity in extracellular regions indicates the release of toxic eosinophil granule proteins and gives further evidence for a causative role of eosinophils with regard to structural changes in eosinophilic oesophagitis. Immunohistochemistry may serve as a useful diagnostic tool to support the morphological differential diagnosis of eosinophilic oesophagitis and gastro-oesophageal reflux disease.\n                  <\/jats:p>","DOI":"10.1136\/jcp.2005.031922","type":"journal-article","created":{"date-parts":[[2006,3,23]],"date-time":"2006-03-23T20:34:05Z","timestamp":1143146045000},"page":"1175-1180","source":"Crossref","is-referenced-by-count":117,"title":["Eosinophil infiltration and degranulation in oesophageal mucosa from adult patients with eosinophilic oesophagitis: a retrospective and comparative study on pathological biopsy"],"prefix":"10.1136","volume":"59","author":[{"given":"S","family":"Mueller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Aigner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Neureiter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Stolte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2006,3,23]]},"reference":[{"key":"2025120519585705000_59.11.1175.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(83)90004-1"},{"key":"2025120519585705000_59.11.1175.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2003.10.054"},{"key":"2025120519585705000_59.11.1175.3","doi-asserted-by":"publisher","DOI":"10.1007\/BF00402221"},{"key":"2025120519585705000_59.11.1175.4","first-page":"516","volume":"91","year":"1996","journal-title":"Am J Gastroenterol"},{"key":"2025120519585705000_59.11.1175.5","doi-asserted-by":"crossref","first-page":"2925","DOI":"10.4049\/jimmunol.123.6.2925","volume":"123","year":"1979","journal-title":"J Immunol"},{"key":"2025120519585705000_59.11.1175.6","doi-asserted-by":"crossref","unstructured":"Gleich GJ, Glitz DG, Abu-Ghazaleh RI. 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