{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T05:44:57Z","timestamp":1764999897558,"version":"3.46.0"},"reference-count":14,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Onchocerca volvulus<\/jats:italic>\n                      infection is traditionally diagnosed by examination of skin snips for the presence of microfilariae. A disadvantage of this method is the low sensitivity particularly with light or prepatent infection. Serodiagnosis using recombinant-antigen-based assays may provide a more sensitive diagnostic test. An ELISA based on a recombinant antigen OvH3 has previously been validated using sera from endemic areas. This study investigated the role of this ELISA-based assay for use in the serodiagnosis of onchocerciasis in non-endemic areas.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>The ELISA-based assay was tested on sera from untreated patients with known onchocerciasis and on untreated and treated patients with definite or probable onchocerciasis identified from a hospital diagnostic database. The assay was also tested on sera from patients with other helminthic infections to determine the sensitivity and specificity of this assay in a tertiary referral laboratory dealing with sera from a variety of patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The sensitivity and specificity of the OvH3 assay were 93.2% and 93.5%, respectively, when tested on non-endemic patients with clinical diagnosis of onchocerciasis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>This study demonstrates the potential role of the assay as a sensitive and specific test for use in the serodiagnosis of onchocerciasis in a reference laboratory dealing with sera from patients in non-endemic setting.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jcp.2006.037093","type":"journal-article","created":{"date-parts":[[2007,10,12]],"date-time":"2007-10-12T20:12:49Z","timestamp":1192219969000},"page":"347-351","source":"Crossref","is-referenced-by-count":10,"title":["The role of a recombinant hybrid protein based ELISA for the serodiagnosis of\n                    <i>Onchocerca volvulus<\/i>"],"prefix":"10.1136","volume":"61","author":[{"given":"J A","family":"Andrews","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W J","family":"Bligh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P L","family":"Chiodini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J E","family":"Bradley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P N","family":"Nde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Lucius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2007,10,8]]},"reference":[{"key":"2025120521402972000_61.3.347.1","unstructured":"World Health Organization. Expert Committee on Onchocerciasis. Third report. WHO Tech Rep Ser 1987;752."},{"key":"2025120521402972000_61.3.347.2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(85)90321-9"},{"key":"2025120521402972000_61.3.347.3","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(95)90656-8"},{"key":"2025120521402972000_61.3.347.4","doi-asserted-by":"publisher","DOI":"10.1126\/science.2011741"},{"key":"2025120521402972000_61.3.347.5","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(91)90046-9"},{"key":"2025120521402972000_61.3.347.6","doi-asserted-by":"crossref","first-page":"198","DOI":"10.4269\/ajtmh.1993.48.198","article-title":"A sensitive serodiagnostic test for onchocerciasis using a cocktail of recombinant antigens.","volume":"48","author":"Bradley","year":"1993","journal-title":"Am J Trop Med Hyg"},{"key":"2025120521402972000_61.3.347.7","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60219-5"},{"key":"2025120521402972000_61.3.347.8","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(94)90433-2"},{"key":"2025120521402972000_61.3.347.9","doi-asserted-by":"crossref","first-page":"877","DOI":"10.4269\/ajtmh.1998.59.877","article-title":"A cocktail of recombinant Onchocerca volvulus antigens for serological diagnosis with the potential to predict the endemicity of onchocerciasis infection.","volume":"59","author":"Bradley","year":"1998","journal-title":"Am J Trop Med Hyg"},{"key":"2025120521402972000_61.3.347.10","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0169-4758(93)90204-S","article-title":"Improved immunodiagnostic tests to monitor onchocerciasis control programmes \u2013 a multicenter effort.","volume":"9","author":"Ramachandran","year":"1993","journal-title":"Parasitol Today"},{"key":"2025120521402972000_61.3.347.11","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.3.549"},{"key":"2025120521402972000_61.3.347.12","doi-asserted-by":"crossref","first-page":"566","DOI":"10.4269\/ajtmh.2002.66.566","article-title":"Sensitive and specific serodiagnosis of onchocerciasis with recombinant hybrid proteins.","volume":"66","author":"Nde","year":"2002","journal-title":"Am J Trop Med Hyg"},{"key":"2025120521402972000_61.3.347.13","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(94)00204-Z"},{"key":"2025120521402972000_61.3.347.14","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.3.1199"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.2006.037093","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T05:40:41Z","timestamp":1764999641000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.2006.037093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10,8]]},"references-count":14,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2008,2,27]]},"published-print":{"date-parts":[[2008,3]]}},"alternative-id":["10.1136\/jcp.2006.037093"],"URL":"https:\/\/doi.org\/10.1136\/jcp.2006.037093","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[2007,10,8]]}}}