{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T07:17:32Z","timestamp":1767856652500,"version":"3.49.0"},"reference-count":21,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    PCR detects clonal rearrangements of the Ig gene in lymphoproliferative disorders. False negativity occurs in germinal centre\/post-germinal centre lymphomas (GC\/PGCLs) as they display a high rate of somatic hypermutation (SHM), which causes primer mismatching when detecting Ig rearrangements by PCR.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aims:<\/jats:bold>\n                    To investigate the degree of SHM in a group of GC\/PGCLs and assess the rate of false negativity when using BIOMED-2 PCR when compared with previously published strategies.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    DNA was isolated from snap-frozen tissue from 49 patients with GC\/PGCL (23 diffuse large B cell lymphomas (DLBCLs), 26 follicular lymphomas (FLs)) and PCR-amplified for complete (VDJH), incomplete (DJH) and Ig\u03ba\/\u03bb rearrangements using the BIOMED-2 protocols, and compared with previously published methods using consensus primers. Germinal centre phenotype was defined by immunohistochemistry based on CD10, Bcl-6 and MUM-1.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Clonality detection by amplifying Ig rearrangements using BIOMED-2 family-specific primers was considerably higher than that found using consensus primers (74% DLBCL and 96% FL vs 69% DLBCL and 73% FL). Addition of BIOMED-2 DJH rearrangements increased detection of clonality by 22% in DLBCL. SHM was present in VDJH rearrangements from all patients with DLBCL (median (range) 5.7% (2.5\u201313.5)) and FL (median (range) 5.3% (2.3\u201311.9)) with a clonal rearrangement.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    Use of BIOMED-2 primers has significantly reduced the false negative rate associated with GC\/PGCL when compared with consensus primers, and the inclusion of DJH rearrangements represents a potential complementary target for clonality assessment, as SHM is thought not to occur in these types of rearrangements.\n                  <\/jats:p>","DOI":"10.1136\/jcp.2006.038984","type":"journal-article","created":{"date-parts":[[2006,6,30]],"date-time":"2006-06-30T21:03:41Z","timestamp":1151701421000},"page":"524-528","source":"Crossref","is-referenced-by-count":22,"title":["Improved clonality assessment in germinal centre\/post-germinal centre non-Hodgkin\u2019s lymphomas with high rates of somatic hypermutation"],"prefix":"10.1136","volume":"60","author":[{"given":"Mark A","family":"Catherwood","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Patton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edwina","family":"Dobbin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lakshmi","family":"Venkatraman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H Denis","family":"Alexander","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2006,6,30]]},"reference":[{"key":"2025121220434482000_60.5.524.1","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1182\/blood.V78.1.192.192","volume":"78","year":"1991","journal-title":"Blood"},{"key":"2025121220434482000_60.5.524.2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S1525-1578(10)60685-X","volume":"4","year":"2002","journal-title":"J Mol Diagn"},{"key":"2025121220434482000_60.5.524.3","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S0002-9440(10)65132-2","volume":"155","year":"1999","journal-title":"Am J Pathol"},{"key":"2025121220434482000_60.5.524.4","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711690303"},{"key":"2025121220434482000_60.5.524.5","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(94)90085-X"},{"key":"2025121220434482000_60.5.524.6","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(96)90111-2"},{"key":"2025121220434482000_60.5.524.7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1998.tb01446.x"},{"key":"2025121220434482000_60.5.524.8","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1182\/blood.V95.5.1797.005k30_1797_1803","volume":"95","year":"2000","journal-title":"Blood"},{"key":"2025121220434482000_60.5.524.9","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.6.1717"},{"key":"2025121220434482000_60.5.524.10","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.43.11.888"},{"key":"2025121220434482000_60.5.524.11","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.45.9.770"},{"key":"2025121220434482000_60.5.524.12","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"2025121220434482000_60.5.524.13","unstructured":"Jaffe ES, Harris NL, Stein H, et al. 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