{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T03:47:34Z","timestamp":1765597654276,"version":"3.48.0"},"reference-count":26,"publisher":"BMJ","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Cyclin D1 expression is central to mantle cell lymphoma (MCL) biology. The cyclin D1 gene produces two forms of mRNA: long (D1L) and short (D1S) versions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>To study the relationship between histology, cyclin D1 mRNA (transcript) levels, cyclin D1 transcript type, cyclin D1 protein expression by immunohistochemistry (IHC), and proliferation (Ki-67%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>17 MCLs were initially studied for: levels of expression of cyclin D1 transcripts and for cyclin D1 transcript type by reverse-transcriptase PCR; intensity and percentage cyclin D1 protein expression by IHC; and Ki-67% by IHC. The relationship between cyclin D1 protein expression and proliferation was further validated on an independent set of 23 MCLs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>MCLs expressed variable levels of cyclin D1 at both transcript and protein levels. Furthermore, D1L and D1S were the predominant transcripts in 69% and 31% of cases, respectively. While only 9% of cases with dominance of D1L had blastoid histology, 60% of the cases with dominance of the D1S had blastoid features. Furthermore, the levels of D1L showed direct correlation with cyclin D1 protein expression and Ki-67%. Among these cases, and in the independent set of MCLs (n\u200a=\u200a40), the level of cyclin D1 protein expression directly correlated with Ki-67%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>MCLs express variable levels of cyclin D1 transcripts and protein, and have variable proliferation (Ki-67%). Cases with dominance of D1S transcripts are more likely to be of blastoid morphology. There is correlation between D1L transcripts levels, cyclin D1 protein expression and Ki-67%.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jcp.2008.057455","type":"journal-article","created":{"date-parts":[[2008,6,13]],"date-time":"2008-06-13T22:56:40Z","timestamp":1213397800000},"page":"920-927","source":"Crossref","is-referenced-by-count":19,"title":["Correlation of cyclin D1 transcript levels, transcript type and protein expression with proliferation and histology among mantle cell lymphoma"],"prefix":"10.1136","volume":"61","author":[{"given":"R","family":"Shakir","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Ngo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K N","family":"Naresh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2008,6,13]]},"reference":[{"key":"2025121219423381000_61.8.920.1","doi-asserted-by":"crossref","first-page":"3909","DOI":"10.1182\/blood.V89.11.3909","article-title":"A clinical evaluation of the International Lymphoma Study Group classification of non-Hodgkin\u00e2\u0080\u0099s lymphoma.","volume":"89","author":"The Non-Hodgkin\u00e2\u0080\u0099s Lymphoma Classification Project","year":"1997","journal-title":"Blood"},{"key":"2025121219423381000_61.8.920.2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04761.x"},{"key":"2025121219423381000_61.8.920.3","first-page":"1833","article-title":"Cyclin D1 messenger RNA overexpression as a marker for mantle cell lymphoma.","volume":"10","author":"de Boer","year":"1995","journal-title":"Oncogene"},{"key":"2025121219423381000_61.8.920.4","first-page":"2253","article-title":"Significance of cyclin D1 overexpression for the diagnosis of mantle cell lymphoma: a clinicopathologic comparison of cyclin D1-positive MCL and cyclin D1-negative MCL-like B-cell lymphoma.","volume":"95","author":"Yatabe","year":"2000","journal-title":"Blood"},{"key":"2025121219423381000_61.8.920.5","unstructured":"Swerdlow S Berger F Isaacson PG . Mantle cell lymphoma. In: Jaffe E S Harris N L Stein H Vardiman J W , eds. World Health Organization classification of tumours. Pathology & genetics. Tumours of haematopoietic and lymphoid tissues, 1st edn. 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