{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T16:56:43Z","timestamp":1765040203406,"version":"3.44.0"},"reference-count":24,"publisher":"BMJ","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:sec>\n                  <jats:title>Aims:<\/jats:title>\n                  <jats:p>The Southeast Asian (SEA) deletion (\u2212\u2212<jats:sup>SEA<\/jats:sup>) represents the most common determinant causing \u03b1\u200athalassaemia in Southeast Asian countries. The embryonic \u03b6-globin chain has been defined as a marker for the detection of this deletion in adults. The aim of this study was to develop an appropriate low-cost ELISA for \u03b6-globin chain detection as a routine screening test for (\u2212\u2212<jats:sup>SEA<\/jats:sup>) \u03b1 thalassaemia deletion.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods:<\/jats:title>\n                  <jats:p>A sandwich ELISA system for \u03b6-globin chains was established with a pair of \u03b6-globin-specific monoclonal antibodies prepared in-house, and locally made products. Against a gap-PCR method that was taken as the standard, this assay was validated in a cohort study testing a total of 526 individuals comprising patients scheduled for haemoglobinopathy diagnostic analysis and normal individuals. Routine screening of the (\u2212\u2212<jats:sup>SEA<\/jats:sup>) deletion in 300 random student volunteers was conducted using the assay.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results:<\/jats:title>\n                  <jats:p>While the cut-off point was set at a percentage positive value of 30, the sensitivity and specificity of this ELISA method were 100% and 99.24%, respectively. The mean intra-assay and inter-assay coefficients of variation among the different concentrations in the optimised ELISA conditions were 2.1\u201311.4% and 4.3\u201313.2%, respectively. Seventeen of the 300 volunteers sampled were determined by the ELISA to have the (\u2212\u2212<jats:sup>SEA<\/jats:sup>) deletion; these results were in 100% agreement with the gap-PCR results.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions:<\/jats:title>\n                  <jats:p>This study validates the ELISA method described here as a simple, rapid and cost-effective assay that is potentially adaptable for application in large-scale population screening for this prevalent disorder in SEA areas such as southern China.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jcp.2008.059477","type":"journal-article","created":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T21:39:59Z","timestamp":1233265199000},"page":"147-151","source":"Crossref","is-referenced-by-count":14,"title":["Development and validation of a \u03b6-globin-specific ELISA for carrier screening of the (\u2212\u2212<sup>SEA<\/sup>) \u03b1 thalassaemia deletion"],"prefix":"10.1136","volume":"62","author":[{"given":"L","family":"Tang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W J","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y Q","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X M","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"2025092506071726000_62.2.147.1","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1182\/blood.V73.5.1081.1081","article-title":"A review molecular genetics of the human alpha-globin gene.","volume":"73","author":"Higgs","year":"1989","journal-title":"Blood"},{"key":"2025092506071726000_62.2.147.2","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1182\/blood.V91.7.2213","article-title":"Hydrops fetalis caused by alpha-thalassemia: an emerging health care 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blood.","volume":"74","author":"Chui","year":"1989","journal-title":"Blood"},{"key":"2025092506071726000_62.2.147.14","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1182\/blood.V80.2.517.517","article-title":"Human embryonic zeta-globin chain expression in deletional alpha-thalassemias.","volume":"80","author":"Tang","year":"1992","journal-title":"Blood"},{"key":"2025092506071726000_62.2.147.15","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1056\/NEJM198601093140203","article-title":"Embryonic zetaglobin chains in adults: a marker for alpha-thalassemia-1 haplotype due to a greater than 17.5-kb deletion.","volume":"314","author":"Chui","year":"1986","journal-title":"N Engl J Med"},{"key":"2025092506071726000_62.2.147.16","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1182\/blood.V72.5.1589.1589","article-title":"A novel monoclonal antibody based diagnostic test for alpha-thalassemia-1 carriers due to the (\u00e2\u0088\u0092\u00e2\u0088\u0092SEA\/) 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Immunoassay San Diego: Academic Press, 1996:227\u00e2\u0080\u009336.","DOI":"10.1016\/B978-012214730-2\/50011-X"},{"key":"2025092506071726000_62.2.147.24","doi-asserted-by":"publisher","DOI":"10.1002\/pd.81"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.2008.059477","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T13:07:22Z","timestamp":1758805642000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.2008.059477"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,29]]},"references-count":24,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2009,1,29]]},"published-print":{"date-parts":[[2009,2]]}},"alternative-id":["10.1136\/jcp.2008.059477"],"URL":"https:\/\/doi.org\/10.1136\/jcp.2008.059477","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[2009,1,29]]}}}