{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T08:15:15Z","timestamp":1765008915903,"version":"3.46.0"},"reference-count":10,"publisher":"BMJ","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2009,3,17]]},"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background and Aim:<\/jats:title>\n                    <jats:p>The cytogenetic and diagnostic hallmark of mantle cell lymphoma (MCL) is translocation t(11;14)(q13;q32), resulting in overexpression of cyclin D1. Cyclin D1 expression was analysed in 32 cases of MCL.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>The t(11;14) translocation was detected by fluorescence in situ hybridisation, level of cyclin D1 mRNA by competitive RT-PCR, and level of cyclin D1 and D2 proteins by immunohistochemistry and\/or immunoblotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>In 30 cases, the presence of translocation t(11;14), a high level of cyclin D1 mRNA, and a high level of the cyclin D1 protein were confirmed. Two cyclin D1-negative cases overexpressing cyclin D2 were detected by immunoblotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>There are rare cyclin D1-negative cases of MCL overexpressing cyclin D2. Anti-cyclin D1 antibodies with low specificity can bind both cyclin D1 and cyclin D2, thus providing false cyclin D1-positive signals in immunohistochemical analysis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jcp.2008.063701","type":"journal-article","created":{"date-parts":[[2009,9,25]],"date-time":"2009-09-25T15:37:06Z","timestamp":1253893026000},"page":"948-950","source":"Crossref","is-referenced-by-count":8,"title":["Complex analysis of cyclin D1 expression in mantle cell lymphoma: two cyclin D1-negative cases detected"],"prefix":"10.1136","volume":"62","author":[{"given":"L","family":"Stefancikova","sequence":"first","affiliation":[{"name":"Department of Pathology, University Hospital Brno. Jihlavska 20, 625 00 Brno, Czech Republic"},{"name":"Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Moulis","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Hospital Brno. Jihlavska 20, 625 00 Brno, Czech Republic"},{"name":"Faculty of Medicine, Masaryk University, Komenskeho namesti 2, 662 43 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Fabian","sequence":"additional","affiliation":[{"name":"Department of Pathology, Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Falkova","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Hospital Brno. Jihlavska 20, 625 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Vasova","sequence":"additional","affiliation":[{"name":"Department of Hematooncology, University Hospital Brno. Jihlavska 20, 625 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Kren","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Hospital Brno. Jihlavska 20, 625 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Macak","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Hospital Brno. Jihlavska 20, 625 00 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Smardova","sequence":"additional","affiliation":[{"name":"Department of Pathology, University Hospital Brno. Jihlavska 20, 625 00 Brno, Czech Republic"},{"name":"Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic"},{"name":"Faculty of Medicine, Masaryk University, Komenskeho namesti 2, 662 43 Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2009,9,25]]},"reference":[{"key":"2025120600124550000_62.10.948.1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2000.02303.x"},{"key":"2025120600124550000_62.10.948.2","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1182\/blood.V89.3.965","article-title":"Detection of cyclin D1 (bcl-1, PRAD1) overexpression by a simple competitive reverse transcription-polymerase chain reaction assay in t(11;14)(q13;q32)-bearing B-cell malignancies and\/or mantle cell lymphoma.","volume":"89","author":"Uchimaru","year":"1997","journal-title":"Blood"},{"key":"2025120600124550000_62.10.948.3","doi-asserted-by":"publisher","DOI":"10.1309\/TYE7K2CQMQ707FRT"},{"key":"2025120600124550000_62.10.948.4","unstructured":"Swerdlow SH Campo E Harris NL , eds. Classification of tumours of haematopoietic and lymphoid tissues. Lyon: IARC Press, 2008."},{"key":"2025120600124550000_62.10.948.5","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-04-1753"},{"key":"2025120600124550000_62.10.948.6","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-10-118794"},{"key":"2025120600124550000_62.10.948.7","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-01-0015"},{"key":"2025120600124550000_62.10.948.8","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(03)00028-X"},{"key":"2025120600124550000_62.10.948.9","first-page":"2253","article-title":"Significance of cyclin D1 overexpression for the diagnosis of mantle cell lymphoma: a clinicopathologic comparison of cyclin D1-positive MCL and cyclin D1-negative MCL-like B-cell lymphoma.","volume":"95","author":"Yatabe","year":"2000","journal-title":"Blood"},{"key":"2025120600124550000_62.10.948.10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.2006.01986.x"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.2008.063701","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T08:12:53Z","timestamp":1765008773000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.2008.063701"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,25]]},"references-count":10,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2009,9,25]]},"published-print":{"date-parts":[[2009,10]]}},"alternative-id":["10.1136\/jcp.2008.063701"],"URL":"https:\/\/doi.org\/10.1136\/jcp.2008.063701","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[2009,9,25]]}}}