{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T17:39:53Z","timestamp":1763746793511,"version":"3.45.0"},"reference-count":27,"publisher":"BMJ","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>A better understanding of the biology of nodal metastatic disease is of indisputable value. Three-dimensional (3D) serial section alignment and reconstruction techniques can be used for visualisation of nodal metastasis and could provide better understanding of disease growth patterns.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>19 tumour-involved sentinel nodes (SLNs) from breast cancer patients were serially sectioned, immunohistochemically stained, and digitally scanned. Digital image alignment and voxel-based rendering was used to construct informative 3D visual representations of metastatic tumour distribution within involved nodes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The 3D reconstruction technique was successful and informative. The reconstructions of all 19 SLNs enabled the metastatic tumour cells to be viewed infiltrating normal SLN tissue from all angles. Metastases were present at the afferent lymphatic pole in 17\/19 cases, confined to the afferent pole only in 7 cases, located at the efferent pole in 12\/19 cases, and efferent pole only in just 2 cases. Finally, this study made the novel observation that metastatic growth occurs in three distinct patterns: sinusoidal, nodular and diffuse.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>This methodology provides improved understanding of metastatic disease development and potentially could be used to develop strategies to improve techniques for its routine detection. Further studies are required in order to evaluate the prognostic and biological significance of the growth patterns identified.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jcp.2009.065219","type":"journal-article","created":{"date-parts":[[2009,3,20]],"date-time":"2009-03-20T20:12:46Z","timestamp":1237579966000},"page":"617-623","source":"Crossref","is-referenced-by-count":13,"title":["Three-dimensional reconstruction of sentinel lymph nodes with metastatic breast cancer indicates three distinct patterns of tumour growth"],"prefix":"10.1136","volume":"62","author":[{"given":"E C","family":"Paish","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A R","family":"Green","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E A","family":"Rakha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R D","family":"Macmillan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J R","family":"Maddison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I O","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2009,3,19]]},"reference":[{"key":"2025112109015514000_62.7.617.1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602794"},{"key":"2025112109015514000_62.7.617.2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.38114.679387.AE"},{"key":"2025112109015514000_62.7.617.3","unstructured":"Stevens A Lowe J . Pathology. Chapter 4. London: Mosby, 1995."},{"key":"2025112109015514000_62.7.617.4","unstructured":"National Health Service Breast Screening Programme. Pathology reporting in breast cancer screening. Publication no. 3. 2nd edn. London: National Health Service, 1995."},{"key":"2025112109015514000_62.7.617.5","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20001215)89:12<2527::AID-CNCR3>3.0.CO;2-6"},{"key":"2025112109015514000_62.7.617.6","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(194807)1:2<238::AID-CNCR2820010208>3.0.CO;2-U"},{"key":"2025112109015514000_62.7.617.7","doi-asserted-by":"publisher","DOI":"10.1097\/01.MP.0000092952.21794.AD"},{"key":"2025112109015514000_62.7.617.8","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19990915)86:6<990::AID-CNCR14>3.0.CO;2-D"},{"key":"2025112109015514000_62.7.617.9","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199903000-00003"},{"key":"2025112109015514000_62.7.617.10","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.5858\/2000-124-1670-EOASLN","article-title":"Evaluation of axillary sentinel lymph node biopsy by immunohistochemistry and multilevel sectioning in patients with breast carcinoma.","volume":"124","author":"Liu","year":"2000","journal-title":"Arch Pathol Lab Medicine"},{"key":"2025112109015514000_62.7.617.11","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(20000301)88:5<1099::AID-CNCR22>3.0.CO;2-7"},{"key":"2025112109015514000_62.7.617.12","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2003.014308"},{"key":"2025112109015514000_62.7.617.13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1075-122X.2004.21283.x"},{"key":"2025112109015514000_62.7.617.14","doi-asserted-by":"publisher","DOI":"10.1309\/V0VC-25AF-09VY-T6Q1"},{"key":"2025112109015514000_62.7.617.15","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200303000-00012"},{"key":"2025112109015514000_62.7.617.16","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(94)90165-1"},{"key":"2025112109015514000_62.7.617.17","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050112"},{"key":"2025112109015514000_62.7.617.18","first-page":"287","article-title":"Evaluation of the prognostic value of morphometric features and cellular DNA content in FIGO1 ovarian cancer patients.","volume":"9","author":"Baak","year":"1987","journal-title":"Anal Quant Cytol Histol"},{"key":"2025112109015514000_62.7.617.19","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199708)248:4<583::AID-AR10>3.0.CO;2-L"},{"key":"2025112109015514000_62.7.617.20","doi-asserted-by":"publisher","DOI":"10.1053\/hp.2000.6536"},{"key":"2025112109015514000_62.7.617.21","first-page":"34a","article-title":"Three dimensional reconstruction of breast carcinomas using routine laboratory equipment and immunohistochemistry.","volume":"210","author":"Jones","year":"2006","journal-title":"J Pathol"},{"key":"2025112109015514000_62.7.617.22","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(00)01611-6"},{"key":"2025112109015514000_62.7.617.23","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2004.024794"},{"key":"2025112109015514000_62.7.617.24","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20010615)91:12<2263::AID-CNCR1257>3.0.CO;2-5"},{"key":"2025112109015514000_62.7.617.25","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(1984)54:4<605::AID-CNCR2820540402>3.0.CO;2-6"},{"key":"2025112109015514000_62.7.617.26","doi-asserted-by":"crossref","unstructured":"Maddison J Paish EC Kurien T . 3D reconstruction and visualisation for exploration of human axillary lymph nodes. Proceedings of the Third International Conference on Medical Information Visualisation \u00e2\u0080\u0093 BioMedical Visualisation. Washington, DC: IEEE Computer Society, 5\u00e2\u0080\u00937 July, 2005:55\u00e2\u0080\u00939.","DOI":"10.1109\/MEDIVIS.2005.2"},{"key":"2025112109015514000_62.7.617.27","doi-asserted-by":"crossref","unstructured":"Paish EC Green AR Rakha EA . Three-dimensional reconstruction of sentinel lymph nodes with metastatic breast cancer indicates three distinct patterns of tumour growth. http:\/\/www.nottingham.ac.uk\/~mrzarg\/Paish_figures.htm (accessed 22 April 2009).","DOI":"10.1136\/jcp.2009.065219"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.2009.065219","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T17:02:05Z","timestamp":1763744525000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.2009.065219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3,19]]},"references-count":27,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2009,6,26]]},"published-print":{"date-parts":[[2009,7]]}},"alternative-id":["10.1136\/jcp.2009.065219"],"URL":"https:\/\/doi.org\/10.1136\/jcp.2009.065219","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[2009,3,19]]}}}