{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T19:50:25Z","timestamp":1760385025919,"version":"3.44.0"},"reference-count":35,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The prevalence of <jats:italic>Trichomonas vaginalis<\/jats:italic> is not accurately estimated, since it is not a reportable disease.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>To assess the prevalence of <jats:italic>T vaginalis<\/jats:italic> infection in women attending a Greek gynaecological hospital and to evaluate four diagnostic methods for <jats:italic>T vaginalis<\/jats:italic> infection.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>255 symptomatic and 247 asymptomatic women were included in the study during 2006\u201307; 372 were Greek and 130 were immigrants. <jats:italic>T vaginalis<\/jats:italic> was detected in vaginal samples, using wet mount, culture in modified Diamond's medium, antigen detection and two PCR assays, targeting different regions of <jats:italic>T vaginalis<\/jats:italic> genome. Specimens were considered positive for <jats:italic>T vaginalis<\/jats:italic>, when tested positive either by culture or by both PCRs.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>23 women (4.6%) were positive for <jats:italic>T vaginalis<\/jats:italic>. Seven of the 23 positive samples (30.4%) were only PCR-positive. Infection was more prevalent in symptomatic women (6.7%) than in asymptomatic ones (2.4%). <jats:italic>T vaginalis<\/jats:italic> was more frequently detected in immigrants (7.9%) than in Greek women (3.3%). <jats:italic>Gardnerella vaginalis<\/jats:italic> infection was significantly more frequent in women infected with <jats:italic>T vaginalis<\/jats:italic>. PCR was the most sensitive method (100%), followed by culture (69.6%), wet mount (69.6%) and latex agglutination (54.6%). Agreement between PCR and culture as well as wet mount examination was very good (\u03ba=0.79).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The study shows a relatively low percentage of trichomoniasis in the female population living in Athens. The infection was more prevalent among immigrants, and a proportion of the infected women was asymptomatic. The tested methods had good agreement and PCR was found to improve the diagnostic yield considerably.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jcp.2009.070920","type":"journal-article","created":{"date-parts":[[2010,3,4]],"date-time":"2010-03-04T11:19:48Z","timestamp":1267701588000},"page":"249-253","source":"Crossref","is-referenced-by-count":14,"title":["Prevalence of <i>Trichomonas vaginalis<\/i> infection in women attending a major gynaecological hospital in Greece: a cross-sectional study"],"prefix":"10.1136","volume":"63","author":[{"given":"Evangelia-Theophano","family":"Piperaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianna","family":"Theodora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Mendris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louisa","family":"Barbitsa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassiliki","family":"Pitiriga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aris","family":"Antsaklis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Athanassios","family":"Tsakris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2010,3,4]]},"reference":[{"key":"2025092505063921000_63.3.249.1","unstructured":"Krieger J . 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