{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T11:19:33Z","timestamp":1765711173359,"version":"3.48.0"},"reference-count":10,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2009,11,23]]},"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:p>\n                    Myeloid sarcoma of the breast is a rare manifestation of acute myeloid leukaemia (AML). This report describes a patient who was diagnosed with AML FAB M2. Molecular analysis showed evidence of an\n                    <jats:italic>NPM1<\/jats:italic>\n                    mutation (subtype A) and internal tandem duplications of the\n                    <jats:italic>FLT3<\/jats:italic>\n                    gene (\n                    <jats:italic>FLT3<\/jats:italic>\n                    -ITD). Eight months after allogeneic stem cell transplantation, the patient developed a palpable mass in the left breast initially suspected as breast carcinoma. Core needle biopsy of the lesion resulted in diagnosis of myeloid sarcoma. Molecular analysis of formalin-fixed specimens of the breast tumour confirmed the known\n                    <jats:italic>FLT3<\/jats:italic>\n                    and\n                    <jats:italic>NPM1<\/jats:italic>\n                    gene mutations. Immunohistochemically, an aberrant cytoplasmic staining pattern for\n                    <jats:italic>NPM1<\/jats:italic>\n                    and overexpression of\n                    <jats:italic>FLT3<\/jats:italic>\n                    were demonstrated. The myeloid sarcoma showed complete transient resolution following treatment with the kinase inhibitor sorafenib. However, the patient developed bone marrow relapse and died in fatal cerebral haemorrhage 1\u2005year after initial diagnosis of AML. In summary, combined molecular and immunohistochemical examination of\n                    <jats:italic>NPM1<\/jats:italic>\n                    and\n                    <jats:italic>FLT3<\/jats:italic>\n                    is helpful in the diagnosis of extramedullary manifestations of AML in core needle biopsies.\n                  <\/jats:p>","DOI":"10.1136\/jcp.2009.071357","type":"journal-article","created":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T20:36:09Z","timestamp":1270154169000},"page":"558-561","source":"Crossref","is-referenced-by-count":8,"title":["Immunohistochemistry and molecular analyses in myeloid sarcoma of the breast in a patient with relapse of\n                    <i>NPM1<\/i>\n                    -mutated and\n                    <i>FLT3<\/i>\n                    -mutated AML after allogeneic stem cell transplantation"],"prefix":"10.1136","volume":"63","author":[{"given":"Matthias","family":"Choschzick","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrike","family":"Bacher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis","family":"Ayuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette","family":"Lebeau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2010,4,1]]},"reference":[{"key":"2025121403171030000_63.6.558.1","unstructured":"Pileri SA Orazi A Falini B . Myeloid sarcoma. In: Swerdlow SH Campo E Lee Harris N , eds. WHO classification of tumours of haematopoietic and lymphoid tissues. 4th Edition. Lyon: IARCPress, 2008:140\u00e2\u0080\u00931."},{"key":"2025121403171030000_63.6.558.2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-1596-2-42"},{"key":"2025121403171030000_63.6.558.3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa041974"},{"key":"2025121403171030000_63.6.558.4","first-page":"1911","article-title":"Internal tandem duplication of the flt3 gene found in acute myeloid leukemia","volume":"10","author":"Nakao","year":"1996","journal-title":"Leukemia"},{"key":"2025121403171030000_63.6.558.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2008.09.014"},{"key":"2025121403171030000_63.6.558.6","doi-asserted-by":"publisher","DOI":"10.1080\/10428190600967022"},{"key":"2025121403171030000_63.6.558.7","doi-asserted-by":"publisher","DOI":"10.1177\/106689690301100404"},{"key":"2025121403171030000_63.6.558.8","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800768"},{"key":"2025121403171030000_63.6.558.9","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-007-0323-5"},{"key":"2025121403171030000_63.6.558.10","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djm328"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.2009.071357","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T11:17:20Z","timestamp":1765711040000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.2009.071357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4,1]]},"references-count":10,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2010,5,24]]},"published-print":{"date-parts":[[2010,6]]}},"alternative-id":["10.1136\/jcp.2009.071357"],"URL":"https:\/\/doi.org\/10.1136\/jcp.2009.071357","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[2010,4,1]]}}}