{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T09:09:21Z","timestamp":1765012161915,"version":"3.46.0"},"reference-count":16,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To improve the detection of\n                      <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n                      by designing a multiplex PCR assay using two\n                      <jats:italic>N gonorrhoeae<\/jats:italic>\n                      -specific genes as targets, thereby providing detection and confirmation of a positive result simultaneously.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      PCR primers were designed to detect two\n                      <jats:italic>N gonorrhoeae<\/jats:italic>\n                      genes, namely porA and pgi1. Primers for an internal control targeting the\n                      <jats:italic>ompW<\/jats:italic>\n                      gene of\n                      <jats:italic>Vibrio cholerae<\/jats:italic>\n                      were also designed and incorporated in the assay. The DNA of 45 clinical isolates including 33\n                      <jats:italic>N gonorrhoeae<\/jats:italic>\n                      isolates, seven non-gonococcal Neisseria species, and five non-Neisseria species was tested using the multiplex PCR assay.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All 33\n                      <jats:italic>N gonorrhoeae<\/jats:italic>\n                      isolates were successfully detected by the assay and none of the non-gonococcal isolates was detected. The assay showed a sensitivity and specificity of 100%, and a limit of detection of 1.25\u2009ng of DNA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This multiplex PCR assay offers a sensitive and specific assay suitable for the detection of\n                      <jats:italic>N gonorrhoeae<\/jats:italic>\n                      , and offers real potential for diagnostic use.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jcp.2009.071431","type":"journal-article","created":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T20:36:09Z","timestamp":1270154169000},"page":"431-433","source":"Crossref","is-referenced-by-count":4,"title":["Design of a multiplex PCR assay for the simultaneous detection and confirmation of\n                    <i>Neisseria 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