{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T10:08:33Z","timestamp":1782382113678,"version":"3.54.5"},"reference-count":31,"publisher":"BMJ","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2010,10,4]]},"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>The BIOMED-2 multiplex PCR protocol is a commonly used procedure for assessing B cell clonality in lymphoma diagnostics. Follicular lymphoma poses a special challenge for PCR-based analyses because of high prevalence of somatic hypermutations in the rearranged immunoglobulin (IG) domains. This study aimed to evaluate the BIOMED-2 protocol performance in detection of B cell clonality in follicular lymphoma using formalin-fixed, paraffin-embedded (FFPE) tissue.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>FFPE samples from 118 patients diagnosed with follicular lymphoma in the period 1998\u20132008 were used in the study. Clonality of IG heavy (<jats:italic>IGH<\/jats:italic>) and light chains (<jats:italic>IGK<\/jats:italic>, <jats:italic>IGL<\/jats:italic>) was assessed using a PCR procedure that was optimised for FFPE tissue.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The highest clonal detection rates were 67.8% with the <jats:italic>IGH<\/jats:italic> V\u043d-FR2-J\u043d assay and 66.1% with the <jats:italic>IGK<\/jats:italic> V\u03ba-J\u03ba assay. Clonality was detected in 94.9% of all FFPE follicular lymphoma samples when all assays were combined. FFPE samples stored for 1\u20135\u2005years did not perform significantly differently from those stored for 6\u201311\u2005years. Interobserver agreement of clonality was tested for all analyses. The lowest score (Cohen's <jats:italic>\u03ba<\/jats:italic> value = 0.56) was observed for the <jats:italic>IGK<\/jats:italic> V\u03ba-J\u03ba clonality assay.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>An improved PCR protocol for detection of clonality in FFPE samples using BIOMED-2 IG primers is presented. For best performance, a combination of <jats:italic>IGH<\/jats:italic> and <jats:italic>IGK<\/jats:italic> analyses is recommended.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jcp.2010.081109","type":"journal-article","created":{"date-parts":[[2010,10,28]],"date-time":"2010-10-28T23:45:46Z","timestamp":1288309546000},"page":"37-41","source":"Crossref","is-referenced-by-count":30,"title":["Detection of clonality in follicular lymphoma using formalin-fixed, paraffin-embedded tissue samples and BIOMED-2 immunoglobulin primers"],"prefix":"10.1136","volume":"64","author":[{"given":"Ellen","family":"Berget","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Helgeland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Molven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olav Karsten","family":"Vintermyr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2010,10,28]]},"reference":[{"key":"2025092504121646000_64.1.37.1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2007.00876.x"},{"key":"2025092504121646000_64.1.37.2","doi-asserted-by":"publisher","DOI":"10.1517\/17530059.1.4.451"},{"key":"2025092504121646000_64.1.37.3","first-page":"98","article-title":"Improvements to B cell clonality analysis using PCR amplification of immunoglobulin light chain genes","volume":"55","author":"Diss","year":"2002","journal-title":"J Clin Pathol"},{"key":"2025092504121646000_64.1.37.4","first-page":"794","article-title":"Comparison of different polymerase chain reaction-based approaches for clonality assessment of immunoglobulin heavy-chain gene rearrangements in B-cell neoplasia","volume":"12","author":"Derksen","year":"1999","journal-title":"Mod Pathol"},{"key":"2025092504121646000_64.1.37.5","first-page":"471","article-title":"Description of a novel FR1 IgH PCR strategy and its comparison with three other strategies for the detection of clonality in B-cell malignancies","volume":"9","author":"Aubin","year":"1995","journal-title":"Leukemia"},{"key":"2025092504121646000_64.1.37.6","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.6.493"},{"key":"2025092504121646000_64.1.37.7","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/j.1365-2141.1990.tb02643.x","article-title":"Development of a highly sensitive assay, based on the polymerase chain reaction, for rare B-lymphocyte clones in a polyclonal population","volume":"75","author":"Brisco","year":"1990","journal-title":"Br J Haematol"},{"key":"2025092504121646000_64.1.37.8","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.43.5.429"},{"key":"2025092504121646000_64.1.37.9","doi-asserted-by":"crossref","first-page":"2220","DOI":"10.1182\/blood.V75.11.2220.2220","article-title":"Monoclonality in B-lymphoproliferative disorders detected at the DNA level","volume":"75","author":"Trainor","year":"1990","journal-title":"Blood"},{"key":"2025092504121646000_64.1.37.10","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"2025092504121646000_64.1.37.11","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404479"},{"key":"2025092504121646000_64.1.37.12","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404482"},{"key":"2025092504121646000_64.1.37.13","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404467"},{"key":"2025092504121646000_64.1.37.14","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.1016\/S0002-9440(10)64472-0","article-title":"Effect of fixatives and tissue processing on the content and integrity of nucleic acids","volume":"161","author":"Srinivasan","year":"2002","journal-title":"Am J Pathol"},{"key":"2025092504121646000_64.1.37.15","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90390-0"},{"key":"2025092504121646000_64.1.37.16","first-page":"345","article-title":"Efficient DNA extraction from 25-year-old paraffin-embedded tissues: study of 365 samples","volume":"39","author":"Gillio-Tos","year":"2007","journal-title":"Pathology (Phila)"},{"key":"2025092504121646000_64.1.37.17","doi-asserted-by":"publisher","DOI":"10.1136\/mp.53.1.51"},{"key":"2025092504121646000_64.1.37.18","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3880232"},{"key":"2025092504121646000_64.1.37.19","unstructured":"Swerdlow SH Campo E Harris NL , eds. 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