{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:03:15Z","timestamp":1761919395108,"version":"3.44.0"},"reference-count":40,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2010,11,30]]},"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>CpG island methylator phenotype (CIMP) involves the targeting of multiple genes by promoter hypermethylation, and the cell-cycle regulatory proteins often change in human neoplasms. To gain insight into the role of epigenetic aberration of cell-cycle regulator genes in oesophagus squamous cell carcinoma (ESCC), the authors determined the hypermethylation profile in ESCC.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The promoter methylation status of nine cell-cycle regulator genes was examined in 50 ESCC, 50 dysplastic tissues and 50 normal epithelial tissues by methylation-specific PCR.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The frequency of promoter methylation was 52% for <jats:italic>p14<\/jats:italic>, 44% for <jats:italic>p15<\/jats:italic>, 50% for <jats:italic>p16<\/jats:italic>, 56% for <jats:italic>p21<\/jats:italic>, 38% for <jats:italic>p27<\/jats:italic>, 8% for <jats:italic>p53<\/jats:italic>, 42% for <jats:italic>p57<\/jats:italic>, 36% for <jats:italic>p73<\/jats:italic>, and 44% for <jats:italic>RB1<\/jats:italic> of 50 ESCC. CIMP+ was detected in 54% (27\/50) of ESCC and 8% (4\/50) of dysplastic tissues; no CIMP+ was present in normal epithelial tissues (p&lt;0.001). The results show that promoter methylation of <jats:italic>p14<\/jats:italic>, <jats:italic>p15<\/jats:italic>, <jats:italic>p16<\/jats:italic>, <jats:italic>p21<\/jats:italic>, <jats:italic>p27<\/jats:italic>, <jats:italic>p57<\/jats:italic> and <jats:italic>p73<\/jats:italic> was far more common in ESCC samples with CIMP+ than those with CIMP\u2212. A significant difference between CIMP status and TNM stage and metastasis was found in ESCC (p&lt;0.05). Patients with ESCC with CIMP+ had poorer 4-year survival than those with CIMP\u2212.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The results suggest that CIMP of a subset of cell-cycle regulatory genes has an important role in the pathogenesis and progression of ESCC.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jcp.2010.082875","type":"journal-article","created":{"date-parts":[[2010,12,17]],"date-time":"2010-12-17T20:46:58Z","timestamp":1292618818000},"page":"246-251","source":"Crossref","is-referenced-by-count":23,"title":["CpG island methylator phenotype of cell-cycle regulators associated with TNM stage and poor prognosis in patients with oesophageal squamous cell 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