{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T09:36:20Z","timestamp":1758706580813},"reference-count":38,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2011,3,11]]},"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Diagnosis of T-cell lymphoproliferation is sometimes challenging, and in certain instances pathologists rely heavily on the clonality assessment results of T-cell receptor (TCR) gene rearrangement (TCR-GR). Many investigators have designed various in-house primer sets for PCR-based study targeting different loci of TCR genes. In recent years, the commercial BIOMED-2 protocols have become available. The in-house primers are very cheap while the BIOMED-2 primers are expensive. This parallel study aimed to compare the sensitivity of the in-house<jats:italic>TCRG<\/jats:italic>primers (two reactions) and the BIOMED-2 TCR primers (six reactions) in an attempt to develop a sensitive and cost-effective strategy for TCR-GR assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>PCR-based analysis was performed on 69 samples of T-lineage neoplasms including 60 formalin-fixed paraffin-embedded (FFPE) tissues, 5 samples from peripheral blood (PB) and 4 samples from bone marrow (BM) aspirate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Forty-seven (78%) FFPE and all PB or BM aspirate samples yielded control DNA products suitable for clonality assessment including 4 precursor and 50 mature T-cell neoplasms. The detection rates of clonal TCR-GR were 63% (34\/54) by the two in-house<jats:italic>TCRG<\/jats:italic>primers, 85% (46\/54) by all six BIOMED-2 reactions, 91% (49\/54) by combining the in-house and BIOMED-2<jats:italic>TCRG<\/jats:italic>reactions and 94% (51\/54) by combining the in-house and all BIOMED-2 reactions. By using the in-house and BIOMED-2<jats:italic>TCRG<\/jats:italic>reactions with a total of four tubes, clonal TCR-GR was detected in 91% of the cases. The reagent cost for this combination was one-third of that for the six BIOMED-2 reactions and the detection rate was also higher than the latter alone (91% vs 85%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>As the in-house primers were custom made and are much cheaper than the commercial kits, the authors concluded that this four-tube strategy was cost-effective and efficient for TCR-GR clonality assessment.<\/jats:p><\/jats:sec>","DOI":"10.1136\/jcp.2010.086637","type":"journal-article","created":{"date-parts":[[2011,4,14]],"date-time":"2011-04-14T04:21:07Z","timestamp":1302754867000},"page":"536-542","source":"Crossref","is-referenced-by-count":18,"title":["A parallel comparison of T-cell clonality assessment between an in-house PCR assay and the BIOMED-2 assay leading to an efficient and cost-effective strategy"],"prefix":"10.1136","volume":"64","author":[{"given":"Szu-Yin","family":"Kuo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongxiang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yung-Liang","family":"Liao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng-Tsung","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yen-Chuan","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Betty Angela Nuako","family":"Bandoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Qing","family":"Du","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih-Sung","family":"Chuang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2011,4,13]]},"reference":[{"key":"2024053014483424000_64.6.536.1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"2024053014483424000_64.6.536.2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(09)70351-1"},{"key":"2024053014483424000_64.6.536.3","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1056\/NEJM198312293092601","article-title":"Immunoglobulin-gene rearrangements as unique clonal markers in human lymphoid neoplasms","volume":"309","author":"Arnold","year":"1983","journal-title":"N Engl J Med"},{"key":"2024053014483424000_64.6.536.4","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1056\/NEJM198508293130902","article-title":"T-cell receptor gene rearrangements as clinical markers of human T-cell lymphomas","volume":"313","author":"Bertness","year":"1985","journal-title":"N Engl J Med"},{"key":"2024053014483424000_64.6.536.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.11.7096"},{"key":"2024053014483424000_64.6.536.6","doi-asserted-by":"crossref","first-page":"2220","DOI":"10.1182\/blood.V75.11.2220.2220","article-title":"Monoclonality in B-lymphoproliferative disorders detected at the DNA level","volume":"75","author":"Trainor","year":"1990","journal-title":"Blood"},{"key":"2024053014483424000_64.6.536.7","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711690303"},{"key":"2024053014483424000_64.6.536.8","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3880232"},{"key":"2024053014483424000_64.6.536.9","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711620304"},{"key":"2024053014483424000_64.6.536.10","first-page":"173","article-title":"A simplified method of detection of clonal rearrangements of the T-cell receptor-gamma chain gene","volume":"1","author":"McCarthy","year":"1992","journal-title":"Diagn Mol Pathol"},{"key":"2024053014483424000_64.6.536.11","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.48.11.1045"},{"key":"2024053014483424000_64.6.536.12","doi-asserted-by":"publisher","DOI":"10.1080\/1042819021000040314"},{"key":"2024053014483424000_64.6.536.13","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404481"},{"key":"2024053014483424000_64.6.536.14","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404479"},{"key":"2024053014483424000_64.6.536.15","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404482"},{"key":"2024053014483424000_64.6.536.16","first-page":"659","article-title":"Molecular immunoglobulin\/T- cell receptor clonality analysis in cutaneous lymphoproliferations. 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