{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:15:19Z","timestamp":1760865319054},"reference-count":38,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1970,4]]},"abstract":"<jats:p>A new method has been developed for the assay of serum pyridoxal using<jats:italic>L. casei.<\/jats:italic>Bound pyridoxal phosphate in serum is converted by acid hydrolysis to pyridoxal for which this organism is specific. This method proved to be considerably more sensitive than other methods so far reported in the literature.<\/jats:p><jats:p>Serum pyridoxal concentrations were measured in 151 control subjects aged 17 to 80 years. The range of concentrations found was 1\u00b75 to 13\u00b75 ng\/ml which compared well with values obtained by most workers measuring pyridoxal phosphate by enzymatic methods. A marked fall with age was confirmed, and levels in women of childbearing age were lower than in men of comparable age. Subnormal serum pyridoxal concentrations were found in 62% of patients with sideroblastic anaemia and in the majority of patients with rheumatoid arthritis, Crohn's disease, coeliac disease, and in pregnant women at term.<\/jats:p>","DOI":"10.1136\/jcp.23.3.232","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T04:50:46Z","timestamp":1181364646000},"page":"232-242","source":"Crossref","is-referenced-by-count":46,"title":["The measurement of serum pyridoxal by a microbiological assay using<i>Lactobacillus casei<\/i>"],"prefix":"10.1136","volume":"23","author":[{"given":"Barbara B.","family":"Anderson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Barbara","family":"Peart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine E.","family":"Fulford-Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1970,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1136\/jcp.21.1.85","article-title":"Effect of light on the Lactobacillus casei microbiological assay","volume":"21","author":"Anderson, B.B.; Cowan, J.D.","year":"1968","unstructured":"Anderson, B. B., and Cowan, J. D. (1968). Effect of light on the Lactobacillus casei microbiological assay. J.clin. Path., 21, 85-87.","journal-title":"J.clin. 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The effect of vitamin supplementation on the urinary excretion of tryptophan metabolites by pregnant women."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1111\/j.1365-2141.1958.tb03846.x","article-title":"The absorption of folic acid","volume":"4","author":"Chanarin, I.; Anderson, B.B.; Mollin, D.L.","year":"1958","unstructured":"Chanarin, I., Anderson, B. B., and Mollin, D. L. (1958). The absorption of folic acid. Brit. J. Haemat., 4, 156-166.","journal-title":"Brit. J. Haemat"},{"key":"ref_9","author":"Dawson, A.M.; Holdsworth, C.D.; Pitcher, C.S.","year":"1964","unstructured":"Dawson, A. M., Holdsworth, C. D., and Pitcher, C.S. (1964)."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1002\/art.1780070303","article-title":"Excretion of tryptophan metabolites by patients with rheumatoid arthritis","volume":"7","author":"Flinn, J.H.; Price, J.M.; Yess, N.; Brown, R.R.","year":"1964","unstructured":"Flinn, J. H., Price, J. M., Yess, N., and Brown, R. R. (1964). Excretion of tryptophan metabolites by patients with rheumatoid arthritis. Arthr. and Rheum., 7, 201-210.","journal-title":"Arthr. and Rheum"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1017\/S0022029900009900","article-title":"The effect of heat on the vitamin B, of mnilk","volume":"26","author":"Gregory, M.E.","year":"1959","unstructured":"Gregory, M. E. (1959). The effect of heat on the vitamin B, of mnilk. J. Dairy Res., 26, 203-220.","journal-title":"J. Dairy Res"},{"key":"ref_12","article-title":"A method of determining pyridoxal phosphate in blood by decarboxylation of L-tyrosine'\"","volume-title":"C(U). Clin.","author":"Hamfelt, A.(1962","unstructured":"Hamfelt, A.(1962). A method of determining pyridoxal phosphate in blood by decarboxylation of L-tyrosine'\" C(U). Clin."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/0009-8981(64)90214-1","article-title":"Age variation of vitamin B6 metabolism in man","volume":"10","author":"Hamfelt, A.","year":"1964","unstructured":"Hamfelt, A. (1964). Age variation of vitamin B6 metabolism in man. Clin. chim. Acta, 10, 48-54.","journal-title":"Clin. chim. Acta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/0003-2697(67)90248-5","article-title":"D-alanine interference in microbiological assays of vitamin B, in human blood","volume":"19","author":"Haskell, B.E.; andWalln6fer, U.","year":"1967","unstructured":"Haskell, B. E., andWalln6fer, U. (1967). D-alanine interference in microbiological assays of vitamin B, in human blood. Analyt. Biochem, 19, 569-577.","journal-title":"Analyt. 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J"},{"key":"ref_17","first-page":"917","article-title":"Recherches sur des carences en vitamine B, pendanltgazstation et dans diveertsats pathologiqueasl'aided'eunpereuve de surcharge en pyridoxine","volume":"16","author":"Karlin, R.; Croizat, P.; Revol, L.; Pommatau, E.V.; iala, J.-J.; Dumont, M.","year":"1968","unstructured":"Karlin, R., Croizat, P., Revol, L., Pommatau, E.V,iala, J.-J., and Dumont, M. (1968). Recherches sur des carences en vitamine B, pendanltgazstation et dans diveertsats pathologiqueasl'aided'eunpereuve de surcharge en pyridoxine. Path. Biol., 16, 917-924.","journal-title":"Path. Biol"},{"key":"ref_18","first-page":"94","article-title":"Effect of vitamin B, depletion on the pyridoxal, pyridoxamine and pyridoxine content of the blood and urine of men","author":"Kelsay, J.; Baysal, A.; Linkswiler, H.","year":"1968","unstructured":"Kelsay, J., Baysal, A., and Linkswiler, H. (1968). 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Haemat"},{"key":"ref_21","first-page":"636653","article-title":"Urinary excretion of pyridoxine and 4-pyridoxic acid in rheumatoid arthritis","volume":"7","author":"McKusick, A.B.; Sherwin, R.W.; Jones, L.G.; Hsu, J.M.","year":"1964","unstructured":"McKusick, A. B., Sherwin, R. W., Jones, L. G., and Hsu, J. M. (1964). Urinary excretion of pyridoxine and 4-pyridoxic acid in rheumatoid arthritis. Arthr. and Rheum., 7, 636653.","journal-title":"Arthr. and Rheum"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1002\/art.1780070606","article-title":"Tryptophan metabolism in rheumatic disease","volume":"7","author":"Pinals, R.S.","year":"1964","unstructured":"Pinals, R. S. (1964). Tryptophan metabolism in rheumatic disease. 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E., and Rannefeld, A. N. (1945). The vitamin B, group."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1099\/00221287-1-2-251","article-title":"The preservation of bacteria by drying","volume":"1","author":"Stamp, Lord","year":"1947","unstructured":"Stamp, Lord (1947). The preservation of bacteria by drying. J. gen. Microbiol., 1, 251-265.","journal-title":"J. gen. Microbiol"},{"key":"ref_29","first-page":"833","article-title":"Methods for the determination of vitamin B, in biological materials","volume":"22","author":"Storvick, C.A.; Peters, J.M.","year":"1964","unstructured":"Storvick, C. A., and Peters, J. M. (1964). Methods for the determination of vitamin B, in biological materials. Vitam. and Horn?., 22, 833-854.","journal-title":"Vitam. and Horn?"},{"key":"ref_30","article-title":"Disturbance of vitamin","author":"Wachstein, M.; Gudaitis, A.","year":"1952","unstructured":"Wachstein, M., and Gudaitis, A. (1952). Disturbance of vitamin"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"350","DOI":"10.3181\/00379727-103-25515","article-title":"Pyridoxal phosphate in plasma and leukocytes of normal and pregnant subjects following B, load tests","volume":"103","author":"Wachstein, M.; Kellner, J.D.; Ortiz, J.M.","year":"1960","unstructured":"Wachstein, M., Kellner, J. D., and Ortiz, J. M. (1960). Pyridoxal phosphate in plasma and leukocytes of normal and pregnant subjects following B, load tests. Proc. Soc. exp. Biol. (N.Y.), 103, 350-353.","journal-title":"Proc. Soc. exp. Biol. (N. Y.)"},{"key":"ref_32","author":"Wada, H.; Morisue, T.; Sakamoto, Y.; Ichihara, K.","year":"1957","unstructured":"Wada, H., Morisue, T., Sakamoto, Y., and Ichihara, K. (1957)."},{"key":"ref_33","article-title":"Determination of plasma pyridoxal phosphate with wheat germ glutamic-aspartic apotransaminase","author":"Walsh, M.P.","year":"1966","unstructured":"Walsh, M. P. (1966). 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We did not specifically question our female control subjects about taking oral contraceptives but in view of the above observations, this cannot be overlooked as a possible reason for the lower serum pyridoxal levels found in young women","author":"Brown, Recently; Rose, Price; Wolf","year":"1969","unstructured":"Recently Brown, Rose, Price and Wolf (1969) and previously Rose (1966) showed evidence of disturbed B6 metabolism, on the basis of the tryptophan loading test, in subjects taking an oral contraceptive. We did not specifically question our female control subjects about taking oral contraceptives but in view of the above observations, this cannot be overlooked as a possible reason for the lower serum pyridoxal levels found in young women."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1038\/210196a0","article-title":"Excretion of xanthurenic acid in the urine of women taking progestogen-oestrogen preparations","volume":"210","author":"Rose, D.P.","year":"1966","unstructured":"Rose, D. P. (1966). Excretion of xanthurenic acid in the urine of women taking progestogen-oestrogen preparations. Nature (Lond.), 210, 196-197.","journal-title":"Nature (Lond.)"},{"issue":"44","key":"ref_38","article-title":"Tryptophan metabolism as affected by anovulatory agents","volume":"166","author":"Brown, R.R.; Rose, D.P.; Price, J.M.; Wolf, H.","year":"1969","unstructured":"Brown, R. R., Rose, D. P., Price, J. M., and Wolf, H. (1969). Tryptophan metabolism as affected by anovulatory agents. Ann. N. Y. Acad. Sci. 166,44.","journal-title":"Ann. N. Y. Acad. Sci"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.23.3.232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T05:23:51Z","timestamp":1717133031000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.23.3.232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,4]]},"references-count":38,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1970,4,1]]},"published-print":{"date-parts":[[1970,4]]}},"alternative-id":["10.1136\/jcp.23.3.232"],"URL":"https:\/\/doi.org\/10.1136\/jcp.23.3.232","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1970,4]]}}}