{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T07:34:34Z","timestamp":1765006474013,"version":"3.46.0"},"reference-count":9,"publisher":"BMJ","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1971,11]]},"abstract":"<jats:p>\n                    The morphology of red cells damaged by 2-aminoethyl\n                    <jats:italic>iso<\/jats:italic>\n                    thiouronium bromide (AET) has been compared to that of PNH cells by transmission and scanning electron microscopy. The main features of the effect of AET as demonstrated by the scanning electron microscope were spherical and deformed cells, with surface craters and relatively large pits. The transmission electron microscope revealed loss of surface structure, an unusual amount of irregularly shaped electrondense material and fine pits in the cell membranes. The paroxysmal nocturnal haemoglobinuria cells showed, by transmission electron microscope, electron-dense material scattered over the cell and fine pitting; by scanning electron microscope, larger craters were seen and swellings protruded from the cell surface. Although the AET changes were not identical with the natural abnormality of paroxysmal nocturnal haemoglobinuria, the two types of cells were sufficiently similar to each other to support the proposition that AET cells have many of the characteristics of paroxysmal nocturnal haemoglobinuria.\n                  <\/jats:p>","DOI":"10.1136\/jcp.24.8.677","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"677-684","source":"Crossref","is-referenced-by-count":5,"title":["Electron microscope study of PNH red cells and AET-treated normal red cells (PNH-like cells)"],"prefix":"10.1136","volume":"24","author":[{"given":"S. M.","family":"Lewis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Lambertenghi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Ferrone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Sirchia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1971,11,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1038\/215402a0","article-title":"Surface ultramicroscopy of human blood cells","volume":"215","author":"Clarke, J.A.; Salsbury, A.J.","year":"1967","unstructured":"Clarke, J. A., and Salsbury, A. J. (1967). Surface ultramicroscopy of human blood cells. Nature (Lond.), 215, 402-404.","journal-title":"Nature (Lond.)"},{"key":"ref_2","first-page":"36","article-title":"Preparation of erythrocyte ghosts by gradual haemolysis in hypotonic aqueous solution","volume":"6","author":"Danon, D.; Nevo, A.; Marikovsky, Y.","year":"1956","unstructured":"Danon, D., Nevo, A., and Marikovsky, Y. (1956). Preparation of erythrocyte ghosts by gradual haemolysis in hypotonic aqueous solution. Bull. Res. Councl. Israel E., 6, 36-39.","journal-title":"Bull. Res. Councl. Israel E"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/B978-1-4831-9928-3.50006-0","article-title":"The scanning electron microscope: Principles and applications in biology and medicine","volume":"12","author":"Hayes, T.L.; Pease, R.F.","year":"1968","unstructured":"Hayes, T. L., and Pease, R. F. (1968). The scanning electron microscope: Principles and applications in biology and medicine. Advanc. biol. med. Phys., 12, 85-137.","journal-title":"Advanc. biol. med. Phys"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1111\/j.1365-2141.1965.tb00118.x","article-title":"Electronmicroscope studies of the red cell in paroxysmal nocturnal haemoglobinuria","volume":"11","author":"Lewis, S.M.; Danon, D.; Marikovsky, Y.","year":"1965","unstructured":"Lewis, S. M., Danon, D., and Marikovsky, Y. (1965). Electronmicroscope studies of the red cell in paroxysmal nocturnal haemoglobinuria. Brit. J. Haemat., 11, 689-695.","journal-title":"Brit. J. Haemat"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/j.1365-2141.1971.tb07072.x","article-title":"The effects of phenothiazines on human erythrocytes: an ultrastructural study","volume":"20","author":"Preston, F.E.; Lee, D.","year":"1971","unstructured":"Preston, F. E., and Lee, D. (1971). The effects of phenothiazines on human erythrocytes: an ultrastructural study. Brit. J. Haemat., 20, 563-566.","journal-title":"Brit. J. Haemat"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1136\/jcp.20.4.603","article-title":"A new method for detecting changes in the surface appearance of human red blood cells","volume":"20","author":"Salsbury, A.J.; Clarke, J.A.","year":"1970","unstructured":"Salsbury, A. J., and Clarke, J. A. (1970). A new method for detecting changes in the surface appearance of human red blood cells. J. clin. Path., 20, 603-610.","journal-title":"J. clin. Path"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1038\/215747a0","article-title":"Immune lysis of AET-treated normal red-cells (PNH-like cells)","volume":"215","author":"Sirchia, G.; Dacie, J.V.","year":"1967","unstructured":"Sirchia, G., and Dacie, J. V. (1967). Immune lysis of AET-treated normal red-cells (PNH-like cells). Nature (Lond.), 215, 747-748.","journal-title":"Nature (Lond.)"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1182\/blood.V25.4.502.502","article-title":"The action of two sulfhydryl compounds on normal human red cells: relationship to red cells of paroxysmal nocturnal hemoglobinuria","volume":"25","author":"Sirchia, G.; Ferrone, S.; Mercuriali, F.","year":"1965","unstructured":"Sirchia, G., Ferrone, S., and Mercuriali, F. (1965). The action of two sulfhydryl compounds on normal human red cells: relationship to red cells of paroxysmal nocturnal hemoglobinuria. Blood, 25, 502-510.","journal-title":"Blood"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1182\/blood.V28.1.98.98","article-title":"Observations on certain enzyme activities of normal human red cells treated with sulfhydryl compounds","volume":"28","author":"Sirchia, G.; Ferrone, S.; Milani, R.; Mercuriali, F.","year":"1966","unstructured":"Sirchia, G., Ferrone, S., Milani, R., and Mercuriali, F. (1966). Observations on certain enzyme activities of normal human red cells treated with sulfhydryl compounds. Blood, 28, 98-101.","journal-title":"Blood"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.24.8.677","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T07:31:42Z","timestamp":1765006302000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.24.8.677"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,11]]},"references-count":9,"journal-issue":{"issue":"8","published-online":{"date-parts":[[1971,11,1]]},"published-print":{"date-parts":[[1971,11]]}},"alternative-id":["10.1136\/jcp.24.8.677"],"URL":"https:\/\/doi.org\/10.1136\/jcp.24.8.677","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1971,11]]}}}