{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T03:12:05Z","timestamp":1768273925778,"version":"3.49.0"},"reference-count":30,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1971,12]]},"abstract":"<jats:p>A study is reported of the estimation of plasma calcium fractions and the calcium-binding affinity of plasma proteins in a total sample of 59 people, which included 29 normal subjects and 30 patients with either hypercalcaemia or hypocalcaemia. It was demonstrated that when the sample was considered as a whole there was a significant correlation between the total plasma calcium concentration and the ultrafiltrable, ionized, and protein-bound calcium fractions and between the ultrafiltrable and ionized fractions. We have also demonstrated that in patients with either hypercalcaemia or hypocalcaemia, including acidotic uraemics, the calcium-binding affinity of the plasma proteins did not differ significantly from that in normal subjects. A significant correlation was also found between the total plasma calcium concentration and the ultrafiltrable, ionized and protein-bound calcium fractions when the normal subjects and the groups of patients with hypercalcaemia and hypocalcaemia due to chronic renal failure were considered as separate groups. The group of patients with hypercalcaemia included patients both with hyperparathyroidism and with hypercalcaemia due to other causes.<\/jats:p>","DOI":"10.1136\/jcp.24.9.856","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"856-866","source":"Crossref","is-referenced-by-count":19,"title":["Plasma calcium fractions and the protein-binding of calcium in normal subjects and in patients with hypercalcaemia and hypocalcaemia"],"prefix":"10.1136","volume":"24","author":[{"given":"M. R.","family":"Wills","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. R.","family":"Lewin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1971,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0009-8981(61)90083-3","article-title":"The use of high centrifugal force in separating plasma calcium into its protein-bound and protein-free fractions","volume":"6","author":"B-een, S.; M.; Freeman","year":"1961","unstructured":"S. B-een, M., and Freeman, (1961a). The use of high centrifugal force in separating plasma calcium into its protein-bound and protein-free fractions. Clin. chinm. Acta, 6, 181-187.","journal-title":"Clin. chinm. Acta"},{"key":"ref_2","article-title":"Plasma calcium distribution in relation to parathyroid function","author":"Breen, M.; Freeman, S.","year":"1961","unstructured":"Breen, M., and Freeman, S. (1961b). Plasma calcium distribution in relation to parathyroid function. Amer.J. Physiol., 200,341-344.","journal-title":"Amer. J. Physiol"},{"key":"ref_3","first-page":"433","article-title":"The binding of calcium by the plasma proteins in hyperparathyroidism","volume":"32","author":"Dale, G.; E., N.; Kellerman, M.","year":"1967","unstructured":"G. Dale, N. E., and Kellerman, M. (1967). The binding of calcium by the plasma proteins in hyperparathyroidism. Clin. Sci., 32, 433-442.","journal-title":"Clin. Sci"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/0009-8981(61)90175-9","article-title":"A determination of ionized calcium in serum by two-wavelength spectrophotometry of a metal indicator","volume":"6","author":"Ettori, J.; Scoggan, S.M.","year":"1961","unstructured":"Ettori, J., and Scoggan, S. M. (1961). A determination of ionized calcium in serum by two-wavelength spectrophotometry of a metal indicator. Clin. chim. Acta, 6, 861-865.","journal-title":"Clin. chim. Acta"},{"key":"ref_5","first-page":"27","article-title":"The ionized, complexed and protein-bound fractions of calcium in plasma","author":"Fanconi, A.; Rose, G.A.","year":"1958","unstructured":"Fanconi, A., and Rose, G. A. (1958). The ionized, complexed and protein-bound fractions of calcium in plasma. Quart. J. Med., 27,463-494.","journal-title":"Quart. J. Med"},{"key":"ref_6","article-title":"Calciunm Metabolism and the Bone","author":"Fourman, P.; Royer, P.","year":"1968","unstructured":"Fourman, P., and Royer, P. (1968). Calciunm Metabolism and the Bone."},{"key":"ref_7","first-page":"2","article-title":"The calcium fractions of plasma in hypercalcaemic conditions and in adult coeliac disease","author":"Fowler, D.I.; Fone, D.J.; Cooke, W.T.","year":"1961","unstructured":"Fowler, D. I., Fone, D. J., and Cooke, W. T. (1961). The calcium fractions of plasma in hypercalcaemic conditions and in adult coeliac disease. 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(1970)."},{"key":"ref_10","first-page":"1","article-title":"Ultrafiltration studies on calcium and phosphorus in human serum","volume":"91","author":"Hopkins, T.; Howard, J.E.; Eisenberg, H.","year":"1952","unstructured":"Hopkins, T., Howard, J. E., and Eisenberg, H. (1952). Ultrafiltration studies on calcium and phosphorus in human serum. Bull. Johns Hopk. Hosp., 91, 1-21.","journal-title":"Bull. Johns Hopk. Hosp"},{"key":"ref_11","first-page":"96","article-title":"'Ober Erfahrungen mit neueren Untersuchungsmethoden bei Mineralstoffwechselstudien. It. lonisiertes Calcium im Blutserum","volume":"213","author":"Kotzaurek, R.","year":"1967","unstructured":"Kotzaurek, R. (1967). 'Ober Erfahrungen mit neueren Untersuchungsmethoden bei Mineralstoffwechselstudien. It. lonisiertes Calcium im Blutserum. Arch. klin. Med. 213, 96-105.","journal-title":"Arch. klin. 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Path., 21, 541-554.","journal-title":"J.clin. Path"},{"key":"ref_30","article-title":"Studies in serum proteins: V. A rapid procedure for the estimation of total protein, true albumin, total globulin, alpha globulin, beta globulin and gamma globulin in 1 0 ml of serum","author":"Wolfson, W.Q.; Cohn, C.; Calvary, E.; Ichiba, F.","year":"1948","unstructured":"Wolfson, W. Q., Cohn, C., Calvary, E., and Ichiba, F. (1948). Studies in serum proteins: V. A rapid procedure for the estimation of total protein, true albumin, total globulin, alpha globulin, beta globulin and gamma globulin in 1 0 ml of serum. Amer."}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.24.9.856","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T13:02:24Z","timestamp":1758805344000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.24.9.856"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,12]]},"references-count":30,"journal-issue":{"issue":"9","published-online":{"date-parts":[[1971,12,1]]},"published-print":{"date-parts":[[1971,12]]}},"alternative-id":["10.1136\/jcp.24.9.856"],"URL":"https:\/\/doi.org\/10.1136\/jcp.24.9.856","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,12]]}}}