{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:21:08Z","timestamp":1758846068913,"version":"3.44.0"},"reference-count":10,"publisher":"BMJ","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1972,10]]},"abstract":"<jats:p>The statement made in some standard textbooks that Benedict's qualitative test gives a green, yellow, or orange-red precipitate with pure solutions of glucose of varying strength has been shown to be incorrect. Pure solutions of glucose give only a bright red precipitate at all concentrations. These changes in the colour of the suspensions are observed with urinary glucose only.<\/jats:p>\n               <jats:p>The difference in the action of glucose in water and in urine has been shown to be mostly due to creatinine and to a small extent to the histidine content of urine. The colour of the precipitate depends not only on the concentration of glucose but also on that of creatinine. An increase in concentration of creatinine tends to make the precipitate more yellow. Histidine has a similar though much smaller effect. Attention has been drawn to possible errors in the semi-quantitative assay of urinary glucose by Benedict's test arising out of variation in concentration of creatinine and histidine.<\/jats:p>","DOI":"10.1136\/jcp.25.10.892","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"892-895","source":"Crossref","is-referenced-by-count":6,"title":["The role of creatinine and histidine in Benedict's qualitative test for reducing sugar in urine"],"prefix":"10.1136","volume":"25","author":[{"given":"B. K.","family":"Sur","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. K.","family":"Shukla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. S.","family":"Agashe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1972,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0065-2423(08)60312-1","article-title":"Amino aciduria","volume":"2","author":"Bigwood, E.J.; Crokaert, R.; Schram, E.; Soupart, P.; Vis, H.","year":"1959","unstructured":"Bigwood, E. J., Crokaert, R., Schram, E., Soupart, P., and Vis, H. (1959). Amino aciduria. Advance. clin. Chem., 2, 201-265.","journal-title":"Advance. clin. Chem"},{"key":"ref_2","first-page":"1965","article-title":"Urinary creatinine excretion as an index of the completeness of 24hour urine collections","volume":"2","year":"1969","unstructured":"Edwards, 0. M., Bayliss, R. I. S., and Millen, S. (1969). Urinary creatinine excretion as an index of the completeness of 24hour urine collections. Lancet, 2, 1965-66.","journal-title":"Lancet"},{"key":"ref_3","article-title":"Urine analysis: chemical examination","volume-title":"Gradwohl's Clinical Laboratory Methods and Diagnosis","volume":"2","author":"Frankel, S.","year":"1963","unstructured":"Frankel, S. (1963). Urine analysis: chemical examination. In Gradwohl's Clinical Laboratory Methods and Diagnosis, 6th ed., vol. 2, edited by S. Frankel and S. Reitman. Mosby, St Louis.","edition":"6th ed"},{"key":"ref_4","first-page":"11","author":"Harper, H.A.","year":"1969","unstructured":"Harper, H. A. (1969). Review of Physiological Chemistry, 12th ed., p. 11.","edition":"12th ed","journal-title":"Review of Physiological Chemistry"},{"key":"ref_5","first-page":"160","article-title":"Chemical Methods in Clinical Medicine","author":"Harrison, G.A.","year":"1957","unstructured":"Harrison, G. A. (1957). Chemical Methods in Clinical Medicine, 4th ed., p. 160. Churchill, London.","edition":"4th ed"},{"key":"ref_6","first-page":"83","article-title":"Hawk's Physiological Chemistry","author":"Oser, B.L.","year":"1965","unstructured":"Oser, B. L. (Ed.) (1965). Hawk's Physiological Chemistry, 14th ed., p. 83. McGraw Hill, New York.","edition":"14th ed"},{"issue":"784","key":"ref_7","article-title":"Creatinine excretion","volume":"1","author":"Pierro, A.; Johnson, R.E.","year":"1970","unstructured":"Pierro, A., and Johnson, R. E. (1970). Creatinine excretion. Lancet, 1,784.","journal-title":"Lancet"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1136\/jcp.25.10.896","article-title":"Mode of action of creatinine on colour of cuprous oxide precipitate in Benedict's qualitative sugar test","volume":"25","author":"Sur, B.K.; Shukla, R.K.; Agashe, V.S.","year":"1972","unstructured":"Sur, B. K., Shukla, R. K., and Agashe, V. S. (1972). Mode of action of creatinine on colour of cuprous oxide precipitate in Benedict's qualitative sugar test. J. clin. Path., 25, 896.898.","journal-title":"J. clin. Path"},{"key":"ref_9","first-page":"161","article-title":"Micro-Analysis in Medical Biochemistry","author":"Wootton, I.D.P.","year":"1964","unstructured":"Wootton, I. D. P. (1964). Micro-Analysis in Medical Biochemistry, 4th ed., p. 161. Churchill, London.","edition":"4th ed"},{"issue":"1254","key":"ref_10","article-title":"Creatinine excretion","volume":"2","author":"Zorab, P.A.; Clark, S.; Harrison, A.","year":"1969","unstructured":"Zorab, P. A., Clark, S., and Harrison, A. (1969). Creatinine excretion. Lancet, 2,1254.","journal-title":"Lancet"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.25.10.892","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:32:37Z","timestamp":1758817957000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.25.10.892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,10]]},"references-count":10,"journal-issue":{"issue":"10","published-online":{"date-parts":[[1972,10,1]]},"published-print":{"date-parts":[[1972,10]]}},"alternative-id":["10.1136\/jcp.25.10.892"],"URL":"https:\/\/doi.org\/10.1136\/jcp.25.10.892","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1972,10]]}}}