{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T04:24:03Z","timestamp":1765081443720,"version":"3.46.0"},"reference-count":20,"publisher":"BMJ","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1973,12]]},"abstract":"<jats:p>\n                    A test is described to distinguish the immunofluorescence seen on\n                    <jats:italic>T. pallidum<\/jats:italic>\n                    in syphilis from that due to antibodies against DNA.\n                  <\/jats:p>\n                  <jats:p>\n                    The method consists of comparing the results of the fluorescent treponemal antibody (FTA-ABS) test with the staining obtained with\n                    <jats:italic>T. gondii<\/jats:italic>\n                    and\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    using an indirect fluorescent technique. The ability of a particular serum to stain other organisms, in addition to\n                    <jats:italic>T. pallidum<\/jats:italic>\n                    , at a significant titre indicates that the treponemal fluorescence is not specific, and the loss of this staining property when organisms are pretreated with deoxyribonuclease suggests that the common microbial antigen is DNA. The isolated treponemal fluorescence found in syphilis is unaffected by pretreatment of\n                    <jats:italic>T. pallidum<\/jats:italic>\n                    .\n                  <\/jats:p>\n                  <jats:p>When 123 sera from patients with collagen disorders were examined, non-specific bacterial fluorescence was observed in six sera, while in 95 sera from patients with lepromatous leprosy, a positive FTA-ABS reaction was obtained in 14. There was no accompanying immunofluorescence on other protozoa. Therefore the antibody reacting in the FTA-ABS test in leprosy is likely to be treponemal in origin. A mitochondrial antibody was found in seven out of the 15 sera from patients with lepromatous leprosy.<\/jats:p>","DOI":"10.1136\/jcp.26.12.968","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"968-972","source":"Crossref","is-referenced-by-count":5,"title":["The significance of the fluorescent treponemal antibody (FTA-ABS) test in collagen disorders and leprosy"],"prefix":"10.1136","volume":"26","author":[{"given":"D. J. M.","family":"Wright","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1973,12,1]]},"reference":[{"key":"ref_1","first-page":"28","article-title":"Le diagnostic des borrelioses par immunofluorescence","volume":"111","author":"Allinne, M.; Marx, R.","year":"1966","unstructured":"Allinne, M., and Marx, R. (1966). Le diagnostic des borrelioses par immunofluorescence. Ann. Inst. Pasteur, 111, Suppl., 28-35.","journal-title":"Ann. Inst. Pasteur"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1038\/204194a0","article-title":"Antigenicity of trypanosome nuclei: Evidence that DNA is not coupled to histone in these protozoa","volume":"204","author":"Beck, J.S.; Walker, P.J.","year":"1964","unstructured":"Beck, J. S., Walker, P. J. (1964). Antigenicity of trypanosome nuclei: Evidence that DNA is not coupled to histone in these protozoa. 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J., Brouwer, R., Feltkamp, T. E. W., Ten Veen, J. H., and Van Loghem, J. J. (1972). Measles antibodies in sera from patients with autoimmune disease. Lancet, 1, 115-116.","journal-title":"Lancet"},{"key":"ref_10","article-title":"Manual of Tests for Syphilis (revised) (Public Health Service Publication, No. 411). U. S. Government Printing Office","author":"National Communicable Disease Centre","year":"1969","unstructured":"National Communicable Disease Centre (1969). Manual of Tests for Syphilis (revised) (Public Health Service Publication, No. 411). U.S. Government Printing Office, Atlanta, Georgia and Washington, D.C."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/jid.1966.13","article-title":"Treponemal antibodies in non-syphilitic, positive anti-nuclear factor sera","volume":"46","author":"Neblett, T.R.; Burnham, T.K.; Merriam, L.R.; Fine, G.","year":"1966","unstructured":"Neblett, T. R., Burnham, T. K., Merriam, L. R., and Fine, G. (1966). 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(1970). Syphilis and biologic false positive reactors among leprosy patients. Arch. Derm., 101, 328-330.","journal-title":"Arch. Derm"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1001\/archinte.1972.03650010013003","article-title":"Epstein-Barr virus (herpes-type virus) antibodies in connective tissue diseases","volume":"130","author":"Stevens, D.A.; Stevens, M.B.; Newell, G.R.; Levin, P.H.; Waggonner, D.E.","year":"1972","unstructured":"Stevens, D. A., Stevens, M. B., Newell, G. R., Levin, P. H., and Waggonner, D. E. (1972). Epstein-Barr virus (herpes-type virus) antibodies in connective tissue diseases. Arch. intern. Med., 130, 23-28.","journal-title":"Arch. intern. Med"},{"key":"ref_15","first-page":"313","article-title":"Reactivity in the FTA-ABS test of rabbits hyperimmunized with non-pathogenic treponemes","volume":"46","author":"Tringali, G.R.; Cox, P.M.","year":"1970","unstructured":"Tringali, G. R., and Cox, P. M. (1970). 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World Health Organization; Manual ofAuto-immune Serology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/S0140-6736(73)91971-5","article-title":"Autoantibodies in leprosy","volume":"2","author":"Wright, D.J.M.","year":"1973","unstructured":"Wright, D. J. M. (1973). Autoantibodies in leprosy. Lancet, 2, 40.","journal-title":"Lancet"},{"key":"ref_19","first-page":"740","article-title":"New antibody in early syphilis","volume":"1","author":"Wright, D.J.M.; Doniach, D.; Lessof, M.H.; Turk, J.L.; Grimble, A.S.; Catterall, R.D.","year":"1970","unstructured":"Wright, D. J. M., Doniach, D., Lessof, M. H., Turk, J. L., Grimble, A. S., and Catterall, R. D. (1970). New antibody in early syphilis. Lancet, 1, 740-744.","journal-title":"Lancet"},{"key":"ref_20","first-page":"239","article-title":"Syphilitic immunofluorescence in experimental relapsing fever","volume":"49","author":"Wright, D.J.M.; Ginger, C.D.","year":"1973","unstructured":"Wright, D. J. 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