{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T04:24:11Z","timestamp":1778300651717,"version":"3.51.4"},"reference-count":19,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1974,3]]},"abstract":"<jats:p>Cryostat sections from 160 rectal suction biopsies were stained for cholinesterases by the method of Karnovsky and Roots (1964) in an attempt to facilitate the diagnosis of Hirschsprung's disease.<\/jats:p>\n                  <jats:p>The method proved at least as reliable as experienced assessment of paraffin haematoxylin-eosin sections, and appeared to offer the advantages of reduced scanning fatigue and superior demonstration of the increased cholinesterase-positive nerves in Hirschprung's disease.<\/jats:p>\n                  <jats:p>Contrary to the findings of Meier-Ruge (1971) it was not possible to base a diagnosis on mucosal cholinesterase activity.<\/jats:p>","DOI":"10.1136\/jcp.27.3.207","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"207-213","source":"Crossref","is-referenced-by-count":22,"title":["Experience with a cholinesterase histochemical technique for rectal suction biopsies in the diagnosis of Hirschsprung's disease"],"prefix":"10.1136","volume":"27","author":[{"given":"P. H.","family":"Trigg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Belin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Haberkorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. J.","family":"Long","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. H.","family":"Nixon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Plaschkes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Spitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. H.","family":"Willital","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1974,3,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/0022-3468(68)90670-2","article-title":"Ganglion cell distribution in the normal rectum and anal canal: a basis for the diagnosis of Hirschsprung's disease by anorectal biopsy","volume":"3","author":"Aldridge, R.T.; Campbell, P.E.","year":"1968","unstructured":"Aldridge, R. T., and Campbell, P. E. (1968). Ganglion cell distribution in the normal rectum and anal canal: a basis for the diagnosis of Hirschsprung's disease by anorectal biopsy. J. pediat. Surg., 3, 475-490.","journal-title":"J. pediat. Surg"},{"key":"ref_2","article-title":"Experience with rectal suction biopsy in the diagnosis of Hirschsprung's disease","author":"Campbell, P.E.; Noblett, H.R.","year":"1969","unstructured":"Campbell, P. E., and Noblett, H. R. (1969). Experience with rectal suction biopsy in the diagnosis of Hirschsprung's disease."},{"key":"ref_3","article-title":"Personal communication","author":"Carter, R.F.","year":"1972","unstructured":"Carter, R. F. (1972). Personal communication."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1177\/15.10.580","article-title":"Histochemical methods for separate, consecutive, and simultaneous demonstration of acetylcholinesterase and norepinephrine in cryostat sections","volume":"15","author":"El-Badawi, A.; Schenk, E.A.","year":"1967","unstructured":"El-Badawi, A., and Schenk, E. A. (1967). Histochemical methods for separate, consecutive, and simultaneous demonstration of acetylcholinesterase and norepinephrine in cryostat sections. J. Histochem. Cytochem., 15, 580-588.","journal-title":"J. Histochem. Cytochem"},{"key":"ref_5","first-page":"7","article-title":"A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines","volume":"no.","author":"Falck, B.; Owman, C.","year":"1965","unstructured":"Falck, B., and Owman, C. (1965). A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta Univ. lund., Sect. II, no. 7.","journal-title":"Acta Univ. lund., Sect. II"},{"key":"ref_6","first-page":"894","article-title":"Histochemical diagnosis of Hirschsprung's disease","volume":"1","author":"Gannon, B.J.; Burnstock, G.; Noblett, H.R.; Campbell, P.E.","year":"1969","unstructured":"Gannon, B. J., Burnstock, G., Noblett, H. R., and Campbell, P. E. (1969). Histochemical diagnosis of Hirschsprung's disease. (Letter). Lancet, 1, 894.","journal-title":"(Letter). Lancet"},{"key":"ref_7","first-page":"76","article-title":"Histochemistry and the pathology of Hirschsprung's disease","volume":"4","author":"Garrett, J.R.; Howard, E.R.","year":"1969","unstructured":"Garrett, J. R., and Howard, E. R. (1969). Histochemistry and the pathology of Hirschsprung's disease. Proc. roy. microsc. Soc., 4, 76-78.","journal-title":"Proc. roy. microsc. Soc"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1136\/adc.44.235.406","article-title":"Autonomic nerves in rectum and colon in Hirschsprung's disease","volume":"44","author":"Garrett, J.R.; Howard, E.R.; Nixon, H.H.","year":"1969","unstructured":"Garrett, J. R., Howard, E. R., and Nixon, H. H. (1969a). Autonomic nerves in rectum and colon in Hirschsprung's disease. Arch. Dis. Childh., 44, 406-417.","journal-title":"Arch. Dis. 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Microscopic Histochemistry: Principles and Practice. The University of Chicago Press, Chicago."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1177\/12.3.219","article-title":"A 'direct-colouring' thiocholine method for cholinesterases","volume":"12","author":"Karnovsky, M.J.; Roots, L.","year":"1964","unstructured":"Karnovsky, M. J., and Roots, L. (1964). A 'direct-colouring' thiocholine method for cholinesterases. (Letter). J. Histochem. Cytochem., 12, 219-221.","journal-title":"(Letter). J. Histochem. Cytochem"},{"key":"ref_13","author":"Lake, B.D.","year":"1972","unstructured":"Lake, B. D. (1972). Personal communication."},{"key":"ref_14","first-page":"67","article-title":"Das Megacolon. Seine Diagnose und Pathophysiologie","volume":"344","author":"Meier-Ruge, W.","year":"1968","unstructured":"Meier-Ruge, W. (1968). Das Megacolon. Seine Diagnose und Pathophysiologie. Virchows Arch. path. Anat., 344, 67-85.","journal-title":"Virchows Arch. path. 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