{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T02:55:06Z","timestamp":1764989706175,"version":"3.46.0"},"reference-count":26,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1974,3]]},"abstract":"<jats:p>Bone marrow preparations were examined from 80 patients with aplastic anaemia. The degree of cellularity varied greatly and in a third of the cases it was normal or even hypercellular at one site of aspiration. In the severely hypoplastic marrows lymphoid cells were predominant and this situation was associated with a worse prognosis. There was no correlation between marrow lymphoid cell content and blood lymphocyte count but there was an inverse relationship between blood lymphocyte count and marrow erythroblasts and a close direct relationship between the blood neutrophil count and marrow myeloid cell content.<\/jats:p>\n                  <jats:p>In all cases a proportion of the erythroblasts showed morphological abnormalities. These included especially megaloblastic changes and asynchrony of nuclear-cytoplasmic maturation. There were also binucleated cells, internuclear chromatin bridges, intercellular cytoplasmic connexions, nuclear degenerative changes, namely, blurred outlines, irregular shapes, budding and fragmentation, and atypical mitotic figures. These appearances illustrate the extent to which a qualitative defect of erythropoiesis occurs as part of the haematological pattern in aplastic anaemia, and in some cases dominates the bone marrow picture.<\/jats:p>\n                  <jats:p>Similar cytological features were found in all cases, including five patients with Fanconi's anaemia.<\/jats:p>","DOI":"10.1136\/jcp.27.3.231","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"231-241","source":"Crossref","is-referenced-by-count":39,"title":["The bone marrow in aplastic anaemia: Diagnostic and prognostic features"],"prefix":"10.1136","volume":"27","author":[{"given":"B.","family":"Frisch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. M.","family":"Lewis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1974,3,1]]},"reference":[{"key":"ref_1","first-page":"296","article-title":"Haematological effects of chronic benzene poisoning in 217 workers","volume":"28","author":"Aksoy, M.; Dincol, K.; Akgun, T.; Erdem, S.; Dincol, G.","year":"1971","unstructured":"Aksoy, M., Dincol, K., Akgun, T., Erdem, S., and Dincol, G. (1971). Haematological effects of chronic benzene poisoning in 217 workers. Brit. J. indust. Med., 28, 296-302.","journal-title":"Brit. J. indust. Med"},{"key":"ref_2","first-page":"295","article-title":"Early hematological abnormalities associated with chloramphenicol therapy","volume":"36","author":"Beck, E.A.; Beuhler, F.; Armentrout, S.A.; Ludin, H.","year":"1972","unstructured":"Beck, E.A., Beuhler, F., Armentrout, S. A., Ludin, H. (1972). Early hematological abnormalities associated with chloramphenicol therapy. Helv. med. Acta. 36, 295-306.","journal-title":"Helv. med. Acta"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"593","DOI":"10.7326\/0003-4819-76-4-593","article-title":"Pure red cell anemia: immunosuppressive treatment","volume":"76","author":"Bottiger, L.E.; Rausing, A.","year":"1972","unstructured":"Bottiger, L. E., and Rausing, A. (1972). Pure red cell anemia: immunosuppressive treatment. Ann. intern. med., 76, 593-597.","journal-title":"Ann. intern. med"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1111\/j.1365-2141.1968.tb01554.x","article-title":"Increased agglutinability by anti-i of red cells in sideroblastic and megaloblastic anaemia","volume":"15","author":"Cooper, A.G.; Hoffbrand, A.V.; Worlledge, S.M.","year":"1968","unstructured":"Cooper, A. G., Hoffbrand, A. V., and Worlledge, S. M. (1968). Increased agglutinability by anti-i of red cells in sideroblastic and megaloblastic anaemia. Brit. J. Haemat., 15, 381-387.","journal-title":"Brit. J. Haemat"},{"key":"ref_5","article-title":"Practical Haematology","author":"Dacie, J.V.; Lewis, S.M.","year":"1968","unstructured":"Dacie, J. V., and Lewis, S. M. (1968). Practical Haematology, 4th ed. Churchill, London.","edition":"4th ed"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1136\/bmj.2.5764.743","article-title":"Aplastic anaemia, acute myeloblastic leukaemia and oxymetholone","volume":"2","author":"Delamore, 1. W.; Geary, C. G","year":"1971","unstructured":"Delamore, 1. W., and Geary, C. G. (1971). Aplastic anaemia, acute myeloblastic leukaemia and oxymetholone. Brit. med. J., 2, 743-745.","journal-title":"Brit. med. J"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1365-2141.1969.tb00404.x","article-title":"Anomalies of blood group antigens and erythrocyte enzymes in two types of chronic refractory anaemia","volume":"16","author":"Dreyfus, B.; Sultan, C.; Rochant, H.; Mannoni, Salmon C.; P., Cartron; P., J.; Boivin, P.; Galand, C.","year":"1969","unstructured":"Dreyfus, B., Sultan, C., Rochant, H., Salmon. C.. Mannoni, P., Cartron, J. P., Boivin, P., and Galand, C. (1969). Anomalies of blood group antigens and erythrocyte enzymes in two types of chronic refractory anaemia. Brit. J. Haemat., 16, 303-312.","journal-title":"Brit. J. Haemat"},{"key":"ref_8","first-page":"1309","article-title":"Die familiare panmyelopathie","volume":"94","author":"Fanconi, G.","year":"1964","unstructured":"Fanconi, G. (1964). Die familiare panmyelopathie. Schweiz. med. Wschr., 94, 1309-1318.","journal-title":"Schweiz. med. Wschr"},{"key":"ref_9","first-page":"957","article-title":"Disturbances in the blood following exposure to benzol","volume":"26","author":"Goldwater, L.J.","year":"1941","unstructured":"Goldwater, L. J. (1941). Disturbances in the blood following exposure to benzol. J. lab. clin. Med., 26, 957-973.","journal-title":"J. lab. clin. Med"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1182\/blood.V27.4.425.425","article-title":"Inhibition of erythropoiesis by a factor present in the plasma of patients with erythroblastopenia","volume":"27","author":"Jepson, J.H.; Lowenstein, L.","year":"1966","unstructured":"Jepson, J. H., and Lowenstein, L. (1966). Inhibition of erythropoiesis by a factor present in the plasma of patients with erythroblastopenia. Blood, 27, 425-434.","journal-title":"Blood"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/S0140-6736(71)80009-0","article-title":"Aplastic anaemia: a disorder of the bone-marrow sinusoidal microcirculation rather than stem-cell failure?","volume":"1","author":"Knospe, W.H.; Crosby, W.H.","year":"1971","unstructured":"Knospe, W. H., and Crosby, W. H. (1971). Aplastic anaemia: a disorder of the bone-marrow sinusoidal microcirculation rather than stem-cell failure? Lancet, 1, 20-22.","journal-title":"Lancet"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1073\/pnas.58.2.493","article-title":"Studies on red cell aplasia: I Demonstration of a plasma inhibitor to heme synthesis and an antibody to erythroblast nuclei","volume":"58","author":"Krantz, S.B.; Kao, V.","year":"1967","unstructured":"Krantz, S. B., and Kao, V. (1967). Studies on red cell aplasia: I Demonstration of a plasma inhibitor to heme synthesis and an antibody to erythroblast nuclei. Proc. Nat. Acad. Sci. (Wash)., 58, 493-500.","journal-title":"Proc. Nat. Acad. Sci. (Wash)"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1182\/blood.V34.1.1.1","article-title":"Studies in red cell aplasia. II. Report of a second patient with an antibody to erythroblast nuclei and a remission after immunosuppressive therapy","volume":"34","author":"Krantz, S.B.; Kao, V.","year":"1969","unstructured":"Krantz, S. B., and Kao, V. (1969). Studies in red cell aplasia. II. Report of a second patient with an antibody to erythroblast nuclei and a remission after immunosuppressive therapy. 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Aplastic anaemia: problems of diagnosis and of treatment. J. Roy. Coil. Phycns. London., 3, 253-264.","journal-title":"J. Roy. Coil. Phycns. London"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1111\/j.1365-2141.1967.tb08736.x","article-title":"The aplastic anaemia-paroxysmal nocturnal haemoglobinuria syndrome","volume":"13","author":"Lewis, S.M.; Dacie, J.V.","year":"1967","unstructured":"Lewis, S. M., and Dacie, J. V. (1967). The aplastic anaemia-paroxysmal nocturnal haemoglobinuria syndrome. Brit. J. Haemat., 13, 236-251. N","journal-title":"Brit. J. Haemat"},{"key":"ref_18","first-page":"465474","article-title":"Lysis by anti-I in dyserythropoietic anaemias: role of increased uptake of antibody","volume":"18","author":"Lewis, S.M.; Grammaticos, P.; Dacie, J.V.","year":"1970","unstructured":"Lewis, S. M., Grammaticos, P., and Dacie, J. V. (1970). Lysis by anti-I in dyserythropoietic anaemias: role of increased uptake of antibody. Brit. J. Haemat., 18, 465474.","journal-title":"Brit. J. Haemat"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2141.1972.tb03452.x","article-title":"Annotation: Dyserythropoiesis and dyserythropoietic anaemia","volume":"23","author":"Lewis, S.M.; Verwilghen, R.L.","year":"1972","unstructured":"Lewis, S. M., and Verwilghen, R. L. (1972). Annotation: Dyserythropoiesis and dyserythropoietic anaemia. Brit. J. Haemat., 23, 1-4.","journal-title":"Brit. J. Haemat"},{"key":"ref_20","first-page":"153","article-title":"Dyserythropoiesis and dyserythropoietic anaemia","volume":"8","author":"Lewis, S.M.; Verwilghen, R.L.","year":"1973","unstructured":"Lewis, S. M., and Verwilghen, R. L. (1973). Dyserythropoiesis and dyserythropoietic anaemia. Progr. Hemat., 8, Li, F. P., Alter, B. P., and Nathan, D. G. (1972). The mortality of acquired aplastic anemia in children. Blood, 40, 153-162.","journal-title":"Progr. Hemat; The mortality of acquired aplastic anemia in children"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1002\/jcp.1040770216","article-title":"Transformation of granulocytes to macrophages in bone marrow colonies in vitro","volume":"77","author":"Metcalf, D.","year":"1971","unstructured":"Metcalf, D. (1971). Transformation of granulocytes to macrophages in bone marrow colonies in vitro. J. cell Physiol., 77, 277-280.","journal-title":"J. cell Physiol"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1159\/000206664","article-title":"Effects du benzene sur la mitose 6rythroblastique. Investigations a la microcinematographie en contraste de phase","volume":"26","author":"Rondanelli, E.G.; Gorini, P.; Pecorari, D.; Trotta, N.; Colombi, R.","year":"1961","unstructured":"Rondanelli, E. G., Gorini, P., Pecorari, D., Trotta, N., and Colombi, R. (1961). Effects du benzene sur la mitose 6rythroblastique. 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Hemat"},{"key":"ref_25","unstructured":"The February 1974 Issue","article-title":"The February 1974 Issue"},{"key":"ref_26","unstructured":"THE FEBRUARY 1974 ISSUE CONTAINS THE FOLLOWING PAPERS","article-title":"THE FEBRUARY 1974 ISSUE CONTAINS THE FOLLOWING PAPERS"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.27.3.231","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T02:16:15Z","timestamp":1764987375000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.27.3.231"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,3]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1974,3,1]]},"published-print":{"date-parts":[[1974,3]]}},"alternative-id":["10.1136\/jcp.27.3.231"],"URL":"https:\/\/doi.org\/10.1136\/jcp.27.3.231","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1974,3]]}}}