{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:47:08Z","timestamp":1771656428654,"version":"3.50.1"},"reference-count":14,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1977,6]]},"abstract":"<jats:p>A modification of the microimmunofluorescence test to provide a practicable routine serodiagnostic test for detecting and characterising chlamydial infection is described which uses four antigen pools, one of which corresponds with each of the four main clinical and epidemiological types of chlamydial infection. The three subgroup A Chlamydia (Chlamydia trachomatis) pools are: pool 1, hyperendemic trachoma TRIC agent serotypes A, B, and C; pool 2, paratrachoma TRIC agent serotypes D, E, F, G, H, I, and K; pool 3, lymphogranuloma venereum (LGV) agent serotypes L1, L2, and L3. Pool 4 contained four representative isolates of subgroup B Chlamydia (Chlamydia psittaci). For routine purposes sera need be screened only against these four representative antigen pools. This will detect antibody and indicate which clinical and epidemiological type of chlamydial infection is implicated, thereby clearly distinguishing those infections that are due to C. psittaci. The pattern of the cross-reactions may indicate the individual serotype involved, and further titration requiring a maximum of four individual antigens is sufficient to determine the serotype. The slight loss in sensitivity (twofold) is more than compensated for by the reduction in cost and the tenfold increase in the total number of sera which can be examined.<\/jats:p>","DOI":"10.1136\/jcp.30.6.510","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"510-517","source":"Crossref","is-referenced-by-count":143,"title":["Modification of the microimmunofluorescence test to provide a routine serodiagnostic test for chlamydial infection."],"prefix":"10.1136","volume":"30","author":[{"given":"J D","family":"Treharne","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Darougar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B R","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1977,6,1]]},"reference":[{"key":"ref_1","first-page":"1262","article-title":"Type-specific antibodies in human Chlamydia trachomatis infections of the eye","volume":"113","year":"1975","unstructured":"Briones, 0. C., Hanna, L., Jawetz, E., Dawson, C. R., and Ostler, H. B. (1975). Type-specific antibodies in human Chlamydia trachomatis infections of the eye. J. Immunol., 113, 1262-1270.","journal-title":"J. Immunol"},{"key":"ref_2","first-page":"452","article-title":"Results ofmicro-immunofluorescence tests for the detection of type-specific antibody in certain chlamydial infections","volume":"48","author":"Dwyer, R.St C.; Treharne, J.D.; Jones, B.R.; Herring, J.","year":"1972","unstructured":"Dwyer, R. St. C., Treharne, J. D., Jones, B. R, and Herring, J. (1972). Results ofmicro-immunofluorescence tests for the detection of type-specific antibody in certain chlamydial infections. Brit. J. vener. Dis., 48, 452-459.","journal-title":"Brit. J. vener. Dis"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"102","DOI":"10.4049\/jimmunol.108.1.102","article-title":"Titrating and typing of serum antibodies in TRIC infections by immunofluorescence","volume":"108","author":"Hanna, L.; Jawetz, E.; Nabli, B.; Hoshiwara, I.; Ostler, B.; Dawson, C.","year":"1972","unstructured":"Hanna, L., Jawetz, E., Nabli, B., Hoshiwara, I., Ostler, B., and Dawson, C. (1972). Titrating and typing of serum antibodies in TRIC infections by immunofluorescence. J. Immunol., 108, 102-107","journal-title":"J. Immunol"},{"key":"ref_4","first-page":"16","article-title":"The prevention of blindness from trachoma","volume":"95","author":"Jones, B.R.","year":"1975","unstructured":"Jones, B. R. (1975). The prevention of blindness from trachoma. Trans. Ophthal. Soc. U.K., 95, 16-33.","journal-title":"Trans. Ophthal. Soc. U. K"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1177\/003591577406700820","article-title":"Micro-immunofluorescence type-specific serological tests for Chlamydia infection","volume":"67","author":"Jones, B.R.; Treharne, J.D.","year":"1974","unstructured":"Jones, B. R. and Treharne, J. D. (1974). Micro-immunofluorescence type-specific serological tests for Chlamydia infection. Proc. roy. Soc. Med., 67, 735-538.","journal-title":"Proc. roy. Soc. Med"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/infdis\/114.1.1","article-title":"Guinea pig inclusion conjunctivitis virus. I. Isolation and identification as a member of the psittacosis-lymphogranuloma-trachoma group","volume":"114","author":"Murray, E.S.","year":"1964","unstructured":"Murray, E. S. (1964). Guinea pig inclusion conjunctivitis virus. I. Isolation and identification as a member of the psittacosis-lymphogranuloma-trachoma group. J. infect. 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Characterization of some recently isolated strains. J. Bact., 99, 636-638.","journal-title":"J. Bact"},{"key":"ref_9","author":"Treharne, J.D.; Darougar, S.; Jones, B.R.","year":"1973","unstructured":"Treharne, J. D., Darougar, S., and Jones, B. R. (1973)."},{"key":"ref_10","first-page":"289","article-title":"Comparison of serotyping subgroup A chlamydial isolates by a one-way cross-reaction and by a two-way cross-reaction immunofluorescence test","volume-title":"Trachoma and Related Disorders Caused by Chlamydial Agents","author":"Trehame, J.D.; Katzenelson, E.; Davey, S.J.; Gray, S.J.","year":"1971","unstructured":"Trehame, J. D., Katzenelson, E., Davey, S. J., and Gray, S. J. (1971). Comparison of serotyping subgroup A chlamydial isolates by a one-way cross-reaction and by a two-way cross-reaction immunofluorescence test. In Trachoma and Related Disorders Caused by Chlamydial Agents, edited by R. L. Nichols, pp. 289-304. 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T., and Gale, J. L. (1973). Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol., 110, 873-879.","journal-title":"J. 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