{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T10:43:16Z","timestamp":1772707396040,"version":"3.50.1"},"reference-count":40,"publisher":"BMJ","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1979,11]]},"abstract":"<jats:p>The cytopathic changes of herpes simplex virus, varicella-zoster virus, the human polyomaviruses, and the trachoma agent can be recognised in smears prepared from a wide variety of specimens. The morphology of virus-infected cells is described, and the clinical value of these observations is discussed. Cytological diagnosis offers a rapid and convenient method of detecting infection with these viruses which can be confirmed by conventional virological method while the patient is still in the acute stages of the disease. Confirmation can also be made retrospectively by reprocessing cells, identified in the Papanicolaou smear, for electron microscopy. Screening for virus-infected cells in cytological smears is a technique that can be developed in any pathology laboratory prepared to provide a comprehensive cytology service, and it is proving to be a useful tool in clinical diagnosis and applied research.<\/jats:p>","DOI":"10.1136\/jcp.32.11.1075","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"1075-1089","source":"Crossref","is-referenced-by-count":10,"title":["Cytological diagnosis of virus-infected cells in Papanicolaou smears and its application in clinical practice."],"prefix":"10.1136","volume":"32","author":[{"given":"D V","family":"Coleman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1979,11,1]]},"reference":[{"key":"ref_1","first-page":"354","article-title":"Herpes simplex virus infection detected on routine gynecological cell specimens","volume":"13","author":"An, S.H.","year":"1969","unstructured":"An, S. 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Journal of Clinical Pathology, 31, 338-347.","journal-title":"Journal of Clinical Pathology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1136\/jcp.30.11.1015","article-title":"Human papovavirus in Papanicolaou smears of urinary sediment detected by transmission electron microscopy","volume":"30","author":"Coleman, D.V.; Russell, W.J.I.; Hodgson, J.; Pe, Tun; Mowbray, J.F.","year":"1977","unstructured":"Coleman, D. V., Russell, W. J. I., Hodgson, J., Tun Pe, and Mowbray, J. F. (1977). Human papovavirus in Papanicolaou smears of urinary sediment detected by transmission electron microscopy. Journal of Clinical Pathology, 30, 1015-1020.","journal-title":"Journal of Clinical Pathology"},{"key":"ref_10","volume-title":"A comparison of laboratory methods of diagnosis of chlamidial infection. In Trachoma and Related Disorders Caused by Chlamydial Agents (Excerpta Medica International Congress Series, 223)","author":"Darougar, S.; Dwyer, R.St C.; Treharne, J.D.; Harper, I.A.; Garland, J.A.; Jones, B.R.","year":"1971","unstructured":"Darougar, S., Dwyer, R. St. C., Treharne, J. D., Harper, I. A., Garland, J. A., and Jones, B. R. (1971). A comparison of laboratory methods of diagnosis of chlamidial infection. In Trachoma and Related Disorders Caused by Chlamydial Agents (Excerpta Medica International Congress Series, 223), edited by R."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1016\/0002-9394(67)94114-1","article-title":"TRIC agent infections of the eye and genital tract","volume":"63","author":"Dawson, C.R.; Schachter, J.","year":"1967","unstructured":"Dawson, C. R., and Schachter, J. (1967). TRIC agent infections of the eye and genital tract. American Journal of Ophthalmology, 63, 1288-1298.","journal-title":"American Journal of Ophthalmology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1093\/ajcp\/22.5.424","article-title":"A new laboratory aid in the clinical diagnosis of inclusion disease of infancy","volume":"22","author":"Fetterman, G.H.","year":"1952","unstructured":"Fetterman, G. H. (1952). A new laboratory aid in the clinical diagnosis of inclusion disease of infancy. American Journal of Clinical Pathology, 22, 424-425.","journal-title":"American Journal of Clinical Pathology"},{"key":"ref_13","article-title":"New human papovavirus","author":"Gardner, S.D.; Field, A.M.; Coleman, D.V.; Hulme, B.","year":"1971","unstructured":"Gardner, S. D., Field, A. M., Coleman, D. V., and Hulme, B. (1971). 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Lancet, 1, 1257-1260.","journal-title":"Lancet"},{"key":"ref_29","first-page":"2647","article-title":"Degenerative changes in ciliated cells exfoliating from the bronchial epithelium as a cytologic criterion in the diagnosis of diseases of the lung","volume":"56","author":"Papanicolaou, G.N.","year":"1956","unstructured":"Papanicolaou, G. N. (1956). Degenerative changes in ciliated cells exfoliating from the bronchial epithelium as a cytologic criterion in the diagnosis of diseases of the lung. New York State Journal of Medicine, 56, 2647-2650.","journal-title":"New York State Journal of Medicine"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"489","DOI":"10.3181\/00379727-98-24084","article-title":"Ciliocytophthoria: relationship to viral respiratory infections of humans. Proceedings ofthe Society for","volume":"98","author":"Pierce, C.H.; Hirsch, J.G.","year":"1958","unstructured":"Pierce, C. H., and Hirsch, J. G. (1958). 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Journal of Medical Research, 14, 361-392.","journal-title":"Journal of Medical Research"},{"key":"ref_38","first-page":"205","article-title":"Le cytodiagnostic imm6diat en dermatologie","volume":"8","author":"Tzanck, A.","year":"1948","unstructured":"Tzanck, A. (1948). Le cytodiagnostic imm6diat en dermatologie. Annales de Dermatologieet Syphiligraphie, 8, 205-218.","journal-title":"Annales de Dermatologieet Syphiligraphie"},{"key":"ref_39","first-page":"358","article-title":"Primary infection of herpes simplex causing necrotic cervicitis","volume":"22","author":"Willcox, R.R.","year":"1968","unstructured":"Willcox, R. R. (1968). Primary infection of herpes simplex causing necrotic cervicitis. 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