{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T06:53:49Z","timestamp":1780556029141,"version":"3.54.1"},"reference-count":14,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1979,3]]},"abstract":"<jats:p>An enzyme-immunoassay was developed to measure the concentration of serum antibody specific for the secretory antigens released by migrating toxocaral larvae. This technique was evaluated by testing sera from healthy UK adults, and from patients with and without toxocariasis. In 922 healthy adults, 2.6% were found to have elevated specific antibody levels. Elevated values were observed twice as frequently in males as in females but showed no significant regression with age between 20 and 65 years. Of 62 patients with non-toxocaral helminthic infections, all had antitoxocaral antibody levels within the range of values observed in healthy controls and had a mean level which was not significantly elevated. All of 13 patients with clinical toxocariasis had enzyme-linked immunosorbent assay (ELISA) antibody levels above the 100th percentiles of both the healthy population and the helminth-infected group and had a significantly high mean value (p less than 0.001) more than 12 times that of the healthy or infected controls. The high degree of sensitivity and specificity of the toxocariasis enzyme-immunoassay indicates that this new test should be useful in reference immunodiagnostic applications and in large-scale seroepidemiological surveys.<\/jats:p>","DOI":"10.1136\/jcp.32.3.284","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"284-288","source":"Crossref","is-referenced-by-count":256,"title":["Toxocariasis: serological diagnosis by enzyme immunoassay."],"prefix":"10.1136","volume":"32","author":[{"given":"D H","family":"de Savigny","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Voller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A W","family":"Woodruff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1979,3,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1136\/jcp.21.4.449","article-title":"The detection of circulating antibody in human Toxocara infections using the indirect fluorescent antibody test","volume":"21","author":"Bisseru, B.; Woodruff, A.W.","year":"1968","unstructured":"Bisseru, B., and Woodruff, A. W. (1968). The detection of circulating antibody in human Toxocara infections using the indirect fluorescent antibody test. Journal of Clinical Pathology, 21, 449-455.","journal-title":"Journal of Clinical Pathology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1093\/ajcp\/69.1.24","article-title":"Percentile estimates of reference values for fourteen chemical constituents in sera of children and adolescents","volume":"69","author":"Cherian, A.G.; Hill, J.G.","year":"1978","unstructured":"Cherian, A. G., and Hill, J. G. (1978). Percentile estimates of reference values for fourteen chemical constituents in sera of children and adolescents. 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The use of indirect fluorescent antibody tests and in vitro larval precipitate test for detecting specific antibodies","volume":"51","author":"Stevenson, P.; Jacobs, D.E.","year":"1977","unstructured":"Stevenson, P., and Jacobs, D. E. (1977). Toxocara infection in pigs. The use of indirect fluorescent antibody tests and in vitro larval precipitate test for detecting specific antibodies. Journal of Helminthology, 51, 149-154.","journal-title":"Journal of Helminthology"},{"key":"ref_9","author":"Voller, A.; Bidwell, D.E.; Bartlett, A.","year":"1976","unstructured":"Voller, A., Bidwell, D. E., and Bartlett, A. (1976a)."},{"key":"ref_10","first-page":"55","article-title":"Enzyme immunoassays in diagnostic medicine: Theory and practice","volume":"53","unstructured":"Enzyme immunoassays in diagnostic medicine: Theory and practice. 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British Medical Journal, 1, 1576-1579.","journal-title":"British Medical Journal; Infection with animal helminths as a factor causing poliomyelitis and epilepsy"},{"key":"ref_13","doi-asserted-by":"crossref","DOI":"10.1136\/bmj.2.6154.1747-a","article-title":"A study of toxocaral infection in dog breeders","author":"Woodruff, A.W.; Jacobs, D.E.; de Savigny, D.H.","year":"1978","unstructured":"Woodruff, A. W., Jacobs, D. E., and de Savigny, D. H. (1978). A study of toxocaral infection in dog breeders. British Medical Journal, (In press).","journal-title":"British Medical Journal"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1136\/bmj.1.5389.1001","article-title":"Infection with animal helminths","volume":"1","author":"Woodruff, A.W.; Thacker, C.K.; Shah, A.I.","year":"1964","unstructured":"Woodruff, A. W., Thacker, C. K., and Shah, A. I. (1964). Infection with animal helminths. 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