{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:03:35Z","timestamp":1765728215338,"version":"3.48.0"},"reference-count":33,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1981,3]]},"abstract":"<jats:p>One hundred endometrial biopsies of various histological patterns, and material from 10 tubal pregnancies together with their associated uterine decidua, were examined for the presence of human placental lactogen using affinity-purified first and second antibodies and an indirect immunoperoxidase technique. Positive cells in endometrial curettings were seen only in association with an intrauterine pregnancy and morphologically resembled syncytiotrophoblast. Decidua associated with tubal pregnancy, pseudodecidua in progestogen-treated patients, and proliferative, secretory, and basal endometria were all negative. An immunoperoxidase stain for human placental lactogen is a useful marker for intrauterine pregnancy and supports the placental origin of the syncytial giant cells in so-called 'syncytial endometritis'. The technique is of potential value in those endometrial biopsies where pregnancy is suspected but no villi are seen.<\/jats:p>","DOI":"10.1136\/jcp.34.3.303","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"303-307","source":"Crossref","is-referenced-by-count":25,"title":["Immunoperoxidase localisation of human placental lactogen: a marker for the placental origin of the giant cells in 'syncytial endometritis' of pregnancy."],"prefix":"10.1136","volume":"34","author":[{"given":"E","family":"Heyderman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A R","family":"Gibbons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S W","family":"Rosen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1981,3,1]]},"reference":[{"issue":"10","key":"ref_1","first-page":"366","article-title":"Chorioma: a clinical and pathological study","volume":"1910","author":"Ewing, J.","unstructured":"Ewing J. Chorioma: a clinical and pathological study. Surg Gynec Obstet 1910;10:366-92.","journal-title":"Surg Gynec Obstet"},{"key":"ref_2","article-title":"A study of its pathology","author":"Choriocarcinoma, Park W.W.","year":"1971","unstructured":"Park WW. Choriocarcinoma. A study of its pathology. London: William Heinemann, 1971;22."},{"issue":"67","key":"ref_3","first-page":"208","article-title":"The giant cells of the pregnant human uterus","volume":"1960","author":"Boyd, J.D.; Hamilton, W.J.","unstructured":"Boyd JD, Hamilton WJ. The giant cells of the pregnant human uterus. J Obstet Gynaec Br Emp 1960;67:208-17.","journal-title":"J Obstet Gynaec Br Emp"},{"issue":"203-1","key":"ref_4","first-page":"1","article-title":"Ultrastructure of the human placental bed","volume":"112","author":"Robertson, W.B.; Warner, B.","year":"1974","unstructured":"Robertson WB, Warner B. Ultrastructure of the human placental bed. JPath 1974;112:203-1 1.","journal-title":"JPath"},{"key":"ref_5","article-title":"Benign trophoblastic lesions in Mathieu chorionepithelioma registry (hydatidiform mole, syncytial endometri is)","author":"Novak, E.; Seah, C.S.","year":"1954","unstructured":"Novak E, Seah CS. Benign trophoblastic lesions in Mathieu chorionepithelioma registry (hydatidiform mole, syncytial endometri is). Amer J Obstet Gynaec 1954; Immunoperoxidase localisation of human placental lactogen","journal-title":"Amer J Obstet Gynaec; Immunoperoxidase localisation of human placental lactogen"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1136\/jcp.25.2.111","article-title":"The diagnosis of trophoblastic tumours from uterine curettings","volume":"25","author":"Elston, C.W.; Bagshawe, K.D.","year":"1972","unstructured":"Elston CW, Bagshawe KD. The diagnosis of trophoblastic tumours from uterine curettings. J Clin Pathol 1972;25: 111-8.","journal-title":"J Clin Pathol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1002\/path.1700930217","article-title":"The trophoblast of the human chorionic villus","volume":"93","author":"Tighe, JR, Garrod; PR, Curran; R.C.","year":"1967","unstructured":"Tighe JR, Garrod PR, Curran RC. The trophoblast of the human chorionic villus. J Path Bact 1967;93:559-67.","journal-title":"J Path Bact"},{"key":"ref_8","first-page":"47","article-title":"Electron microscopic observations on the formation of syncytiotrophoblast from cytotrophoblast","volume":"17","author":"Okudaira, Y.; Susuki, S.; Okudaira, M.; Hashimoto, T.; Hayakawa, K.","year":"1968","unstructured":"Okudaira Y, Susuki S, Okudaira M, Hashimoto T, Hayakawa K. Electron microscopic observations on the formation of syncytiotrophoblast from cytotrophoblast. J Electron Microsc 1968;17:47-54. (Japanese text, English summary.)","journal-title":"J Electron Microsc"},{"key":"ref_9","first-page":"3","volume-title":"Trophoblastic invasion of human decidua from 8 to 18 weeks of pregnancy. Placenta","volume":"1","author":"Pijnenborg, R.; Dixon, G.; Robertson, W.B.; Brosens, I.","year":"1980","unstructured":"Pijnenborg R, Dixon G, Robertson WB, Brosens I. Trophoblastic invasion of human decidua from 8 to 18 weeks of pregnancy. Placenta 1980;1:3-19."},{"key":"ref_10","first-page":"209","article-title":"Brosens 1. Ultrastructure of the spiral arteries in the human placental bed at the end of normal pregnancy","volume":"71","author":"F, De Wolf; C, De Wolf-Peeters","year":"1973","unstructured":"De Wolf F, De Wolf-Peeters C, Brosens 1. Ultrastructure of the spiral arteries in the human placental bed at the end of normal pregnancy. Am J Obstet Gynaec 1973;111: 833-48. \" Josimovich JB, MacLaren JA. Presence in the human placenta and term serum of a highly lactogenic substance immunologically related to pituitary growth hormone. Endocrinology 1962;71:209-20.","journal-title":"Am J Obstet Gynaec; 111: 833-48. \" Josimovich JB, MacLaren JA. Presence in the human placenta and term serum of a highly lactogenic substance immunologically related to pituitary growth hormone"},{"key":"ref_11","first-page":"387","article-title":"The chemistry of growth hormone and the lactogenic hormones Rec Prog Horm Res","volume":"29","author":"Niall, H.D.; Hogan, M.L.; Tregear, G.W.; Segre, G.V.; Hwang, P.; Friesen, H.","year":"1973","unstructured":"Niall HD, Hogan ML, Tregear GW, Segre GV, Hwang P, Friesen H. The chemistry of growth hormone and the lactogenic hormones Rec Prog Horm Res 1973;29: 387-416."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/path.1700920217","article-title":"Read CH. Immunofluorescent localisation of a growth hormone-like factor in normal and abnormal syncytiotrophoblast","volume":"92","author":"Currie, A.R.; Beck, J.S.; Ellis, S.T.","year":"1966","unstructured":"Currie AR, Beck JS, Ellis ST, Read CH. Immunofluorescent localisation of a growth hormone-like factor in normal and abnormal syncytiotrophoblast. J Path Bact 1966;92:395-9.","journal-title":"J Path Bact"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1016\/S0140-6736(78)92019-6","article-title":"Immunoperoxidase technique in routine histopathology","volume":"ii:","author":"Heyderman, E.; Neville, A.M.","year":"1978","unstructured":"Heyderman E, Neville AM. Immunoperoxidase technique in routine histopathology. 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Cambridge: Heffer, 1970:182-3."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0002-9378(16)33248-3","article-title":"Decidua: a possible source of amniotic fluid prolactin","volume":"127","author":"Riddick, D.H.; Kusmik, W.F.","year":"1977","unstructured":"Riddick DH, Kusmik WF. Decidua: a possible source of amniotic fluid prolactin. Am J Obstet Gynec 1977;127: 187-90.","journal-title":"Am J Obstet Gynec"},{"key":"ref_27","first-page":"896","article-title":"Location of the protein hormones of the placenta by the immunoperoxidase technique","volume":"83","author":"Gau, G.; Chard, T.","year":"1976","unstructured":"Gau G, Chard T. Location of the protein hormones of the placenta by the immunoperoxidase technique. Br J Obstet Gynaec 1976;83:896-8.","journal-title":"Br J Obstet Gynaec"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0140-6736(76)90731-5","article-title":"Prognostic significance of inappropriate production of pregnancy proteins by breast cancers","volume":"ii:","author":"Horne, C.H.W.; Reid, I.N.; Milne, G.D.","year":"1976","unstructured":"Horne CHW, Reid IN, Milne GD. Prognostic significance of inappropriate production of pregnancy proteins by breast cancers. Lancet 1976;ii:279-82.","journal-title":"Lancet"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1177\/26.4.96175","article-title":"Use of immunocytochemical techniques for the localisation of human placental lactogen","volume":"26","author":"Watkins, W.B.","year":"1978","unstructured":"Watkins WB. Use of immunocytochemical techniques for the localisation of human placental lactogen. J Histochem Cytochem 1978;26:288-92.","journal-title":"J Histochem Cytochem"},{"key":"ref_30","first-page":"119","article-title":"Immunoperoxidase reactions in resin-embedded sections","volume":"2","author":"Heyderman, E.; Monaghan, P.","year":"1979","unstructured":"Heyderman E, Monaghan P. Immunoperoxidase reactions in resin-embedded sections. Invest Cell Pathol 1979;2: 119-22.","journal-title":"Invest Cell Pathol"},{"issue":"2","key":"ref_31","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1002\/ar.1091230202","article-title":"Electron microscopy of the human placenta","volume":"123","author":"Wislocki, G.B.; Dempsey, E.W.","year":"1955","unstructured":"Wislocki GB, Dempsey EW. Electron microscopy of the human placenta. Anat Rec 1955;123\/2:133-67.","journal-title":"Anat Rec"},{"issue":"78\/8","key":"ref_32","first-page":"673","article-title":"Human chorionic somatomammotrophin (HCS): a new index of placental function","volume":"1971","author":"Teoh, E.S.; Spellacy, W.N.; Buhi, W.C.","unstructured":"Teoh ES, Spellacy WN, Buhi WC. Human chorionic somatomammotrophin (HCS): a new index of placental function. J Obstet Gynaec Br Cmwlth 1971:78\/8:673-85.","journal-title":"J Obstet Gynaec Br Cmwlth"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/S0022-5347(17)60958-3","article-title":"Human placental lactogen in the male subject","volume":"107","author":"Payne, R.A.; Ryan, R.J.","year":"1972","unstructured":"Payne RA, Ryan RJ. Human placental lactogen in the male subject. 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