{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:54:24Z","timestamp":1772164464259,"version":"3.50.1"},"reference-count":24,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1982,5]]},"abstract":"<jats:p>The incidence of ascorbic acid (AA) deficiency and its effect on serum ferritin concentration relative to body iron stores was studied in 61 unchelated patients with beta-thalassaemia major. Thirty-nine (64%) of patients had subnormal leucocyte ascorbate concentrations without clinical evidence of scurvy. The lowest leucocyte ascorbate concentrations tended to occur in the most transfused patients. No correlation was found between the units transfused and serum ferritin concentration in the AA-deficient patients but a close correlation (r =  +0.82; p less than 0.005) existed for the AA-replete group. Similarly a close correlation (r = +0.77; p less than 0.005) was obtained between liver iron concentration and serum ferritin in AA-replete patients but only a weak correlation (r = +0.385; p less than 0.025) existed for the AA-deficient group. When AA-deficient patients were treated with ascorbic acid, serum iron and percentage saturation of iron binding capacity rose significantly; serum ferritin rose in 13 of 21 patients despite the simultaneous commencement of desferrioxamine therapy. In contrast all three measurements tended to fall in AA-replete patients with ascorbic acid and desferrioxamine therapy. Thus, AA deficiency is commonly present in beta-thalassaemia patients with iron overload and may give rise to inappropriate serum ferritin concentrations in relation to body iron stores.<\/jats:p>","DOI":"10.1136\/jcp.35.5.487","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"487-491","source":"Crossref","is-referenced-by-count":66,"title":["Effect of ascorbic acid deficiency on serum ferritin concentration in patients with beta-thalassaemia major and iron overload."],"prefix":"10.1136","volume":"35","author":[{"given":"R W","family":"Chapman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M A","family":"Hussain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Gorman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Laulicht","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Politis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D M","family":"Flynn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Sherlock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A V","family":"Hoffbrand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1982,5,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1111\/j.1365-2141.1964.tb00677.x","article-title":"Iron metabolism in scurvy with special reference to erythropoiesis","volume":"10","author":"Bothwell, T.H.; Bradlow, B.A.; Jacobs, P.","year":"1964","unstructured":"Bothwell TH, Bradlow BA, Jacobs P, et al. Iron metabolism in scurvy with special reference to erythropoiesis. Br J Haematol 1964;10:50-8.","journal-title":"Br J Haematol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/j.1365-2141.1970.tb01624.x","article-title":"The relationship between serum iron levels and ascorbic acid stores in siderotic Bantu","volume":"19","author":"Wapnick, A.A.; Bothwell, T.H.; Seftel, M.","year":"1970","unstructured":"Wapnick AA, Bothwell TH, Seftel M. The relationship between serum iron levels and ascorbic acid stores in siderotic Bantu. Br J Haematol 1970;19:271-6.","journal-title":"Br J Haematol"},{"key":"ref_3","first-page":"704","article-title":"Effects of iron overload on ascorbic acid metabolism","volume":"iii:","author":"Wapnick, A.A.; Lynch, SR, Krawitz; P, Seftel; HC, Charlton; RW, Bothwell; T.H.","year":"1968","unstructured":"Wapnick AA, Lynch SR, Krawitz P, Seftel HC, Charlton RW, Bothwell TH. Effects of iron overload on ascorbic acid metabolism. Br MedJ 1968;iii:704-7.","journal-title":"Br MedJ"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.1365-2141.1971.tb07024.x","article-title":"The role of ascorbic acid in the metabolism of storage iron","volume":"20","author":"Lipschitz, D.A.; Bothwell, T.H.; Seftel, H.C.","year":"1971","unstructured":"Lipschitz DA, Bothwell TH, Seftel HC, et al. The role of ascorbic acid in the metabolism of storage iron. Br J Haematol 1971;20:155-63.","journal-title":"Br J Haematol"},{"key":"ref_5","series-title":"Proceedings of the Fourth International Symposium of Proteins of Iron Storage and Transport","article-title":"Iron chelation and the role of ascorbic acid","author":"Schwartz, E.; Cohen, A.","year":"1979","unstructured":"Schwartz E, Cohen A. Iron chelation and the role of ascorbic acid. In: Proceedings of the Fourth International Symposium of Proteins of Iron Storage and Transport. Davos Switzerland, April 1979."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1136\/jcp.26.10.770","article-title":"Serum ferritin concentration and iron stores in normal subjects","volume":"26","author":"Walters, G.O.; Miller, F.M.; Worwood, M.","year":"1973","unstructured":"Walters GO, Miller FM, Worwood M. Serum ferritin concentration and iron stores in normal subjects. J Clin Pathol 1973;26:770-2.","journal-title":"J Clin Pathol"},{"key":"ref_7","first-page":"218","article-title":"Transferrin iron, chelatable iron and ferritin in idiopathic haemochromatosis","volume":"27","author":"Beamish, M.R.; Walker, R.; Miller, F.","year":"1974","unstructured":"Beamish MR, Walker R, Miller F, et al. Transferrin iron, chelatable iron and ferritin in idiopathic haemochromatosis. Br J Haematol 1974;27:218-28.","journal-title":"Br J Haematol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"953","DOI":"10.3109\/00365527809181375","article-title":"The role of serum ferritin in the management of idiopathic haemochromatosis","volume":"13","author":"Batey, R.G.; Hussein, S.; Sherlock, S.; Hoffbrand, A.V.","year":"1978","unstructured":"Batey RG, Hussein S, Sherlock S, Hoffbrand AV. The role of serum ferritin in the management of idiopathic haemochromatosis. Scand J Gastroenterol 1978;13: 953-7.","journal-title":"Scand J Gastroenterol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/S0016-5085(75)80092-8","article-title":"Serum ferritin in patients with iron overload and with acute and chronic liver diseases","volume":"68","author":"Prieto, J.; Barry, M.; Sherlock, S.","year":"1975","unstructured":"Prieto J, Barry M, Sherlock S. Serum ferritin in patients with iron overload and with acute and chronic liver diseases. Gastroenterology 1975;68:525-33.","journal-title":"Gastroenterology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"171","DOI":"10.3109\/10408367909147133","volume":"10","author":"ferritin, Worwood M.Serum","year":"1979","unstructured":"Worwood M. Serum ferritin. CRC Crit Rev Clin Lab Sci 1979;10:171-204.","journal-title":"CRC Crit Rev Clin Lab Sci"},{"key":"ref_11","first-page":"241","article-title":"Serum ferritin in megaloblastic anaemia","volume":"20","author":"Hussein, S.; Laulicht, M.; Hoffbrand, A.V.","year":"1978","unstructured":"Hussein S, Laulicht M, Hoffbrand AV. Serum ferritin in megaloblastic anaemia. Scand J Haematol 1978;20: 241-5.","journal-title":"Scand J Haematol"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1136\/jcp.25.4.326","article-title":"An immunoradiometric assay for ferritin in the serum of normal subjects and patients with iron deficiency and iron overload","volume":"25","author":"Addison, G.M.; Beamish, M.R.; Hales, C.N.; Hodgkins, M.; Jacobs, A.; Llewellin, P.","year":"1972","unstructured":"Addison GM, Beamish MR, Hales CN, Hodgkins M, Jacobs A, Llewellin P. An immunoradiometric assay for ferritin in the serum of normal subjects and patients with iron deficiency and iron overload. J Clin Pathol 1972;25:326-9.","journal-title":"J Clin Pathol"},{"issue":"21","key":"ref_13","first-page":"157","article-title":"The determination of ascorbic acid in white cells. A comparison of WBC ascorbic acid and phenolic acid excretion in elderly patients","volume":"1961","author":"Denson, K.W.; Bowers, E.F.","unstructured":"Denson KW, Bowers EF. The determination of ascorbic acid in white cells. A comparison of WBC ascorbic acid and phenolic acid excretion in elderly patients. Clin Sci 1961:21:157-62.","journal-title":"Clin Sci"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1111\/j.1365-2141.1978.tb01044.x","article-title":"for Standardisation in Haematology (Iron Panel). The measurement of total and unsaturated iron binding capacity in serum. Recommendation for measurement of serum iron in human blood","volume":"38","author":"International Committee","year":"1978","unstructured":"International Committee for Standardisation in Haematology (Iron Panel). The measurement of total and unsaturated iron binding capacity in serum. Recommendation for measurement of serum iron in human blood. Br J Haematol 1978;38:281-94.","journal-title":"Br J Haematol"},{"key":"ref_15","first-page":"977","article-title":"Effect of dose, time and ascorbate on iron excretion after subcutaneous desferrioxamine","volume":"i:","author":"Hussain, M.A.M.; Green, N.; Flynn, D.M.; Hoffbrand, A.V.","year":"1977","unstructured":"Hussain MAM, Green N, Flynn DM, Hoffbrand AV. Effect of dose, time and ascorbate on iron excretion after subcutaneous desferrioxamine. Lancet 1977;i: 977-9.","journal-title":"Lancet"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/S0140-6736(71)90838-5","article-title":"Measurement of liver iron concentration in needle biopsy specimens","volume":"i:","author":"Barry, M.; Sherlock, S.","year":"1971","unstructured":"Barry M, Sherlock S. Measurement of liver iron concentration in needle biopsy specimens. Lancet 1971;i:100-3.","journal-title":"Lancet"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1159\/000198154","article-title":"Ascorbic acid status in idiopathic haemochromatosis","volume":"17","author":"Brissot, P.; Deugnier, Y.; Le Treut, A.","year":"1978","unstructured":"Brissot P, Deugnier Y, Le Treut A, et al. Ascorbic acid status in idiopathic haemochromatosis. Digestion 1978;17:479-87.","journal-title":"Digestion"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1093\/ajcn\/20.6.641","article-title":"Accelerated oxidative catabolism of ascorbic acid in siderotic Bantu","volume":"20","author":"Lynch, SR, Seftel; HC, Torrance; JD, Charlton; RW, Bothwell; T.H.","year":"1967","unstructured":"Lynch SR, Seftel HC, Torrance JD, Charlton RW, Bothwell TH. Accelerated oxidative catabolism of ascorbic acid in siderotic Bantu. Am J Clin Nutr 1967; 20:641-7.","journal-title":"Am J Clin Nutr"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1136\/jcp.27.8.652","article-title":"Serum ferritin in children with thalassaemia regularly transfused","volume":"27","author":"Letsky, E.A.; Miller, F.; Worwood, M.; Flynn, D.M.","year":"1974","unstructured":"Letsky EA, Miller F, Worwood M, Flynn DM. Serum ferritin in children with thalassaemia regularly transfused. J Clin Pathol 1974;27:652-5.","journal-title":"J Clin Pathol"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1056\/NEJM198101153040311","volume":"304","author":"C, Nienhuis A.S.Vitamin; iron","year":"1981","unstructured":"Nienhuis AS. Vitamin C and iron. N Engl J Med 1981; 304:170-1.","journal-title":"N Engl J Med"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/S0140-6736(79)91721-5","article-title":"Improvement in iron status and liver function in patients with transfusional iron overload with long-term subcutaenous desferrioxamine","volume":"i:","author":"Hoffbrand, A.V.; Gorman, A.; Laulicht, M.","year":"1979","unstructured":"Hoffbrand AV, Gorman A, Laulicht M, et al. Improvement in iron status and liver function in patients with transfusional iron overload with long-term subcutaenous desferrioxamine. Lancet 1979;i:947-9.","journal-title":"Lancet"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1111\/j.1365-2141.1980.tb05987.x","article-title":"Binding of serum ferritin to concanavalin A: patients with homozygous,-thalassaemia and transfusional iron overload","volume":"46","author":"Worwood, M.; Cragg, S.J.; Jacobs, A.","year":"1980","unstructured":"Worwood M, Cragg SJ, Jacobs A, et al. Binding of serum ferritin to concanavalin A: patients with homozygous,-thalassaemia and transfusional iron overload. Br J Haematol 1980;46:409-16.","journal-title":"Br J Haematol"},{"key":"ref_23","first-page":"457","article-title":"Serum ferritin in ascorbic acid deficiency","volume":"45","author":"Roeser, H.P.; Halliday, J.W.; Sizemore, D.J.; Nikles, A.; Willgoss, D.","year":"1980","unstructured":"Roeser HP, Halliday JW, Sizemore DJ, Nikles A, Willgoss D. Serum ferritin in ascorbic acid deficiency. Br J Haematol 1980;45:457-66.","journal-title":"Br J Haematol"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1056\/NEJM198101153040307","article-title":"Scurvy and altered iron stores in thalassaemia major","volume":"304","author":"Cohen, A.; Cohen, L.; Schwartz, E.","year":"1981","unstructured":"Cohen A, Cohen L, Schwartz E. Scurvy and altered iron stores in thalassaemia major. N Engl J Med 1981;304: 158-60.","journal-title":"N Engl J Med"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.35.5.487","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T17:21:38Z","timestamp":1758907298000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.35.5.487"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,5]]},"references-count":24,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1982,5,1]]},"published-print":{"date-parts":[[1982,5]]}},"alternative-id":["10.1136\/jcp.35.5.487"],"URL":"https:\/\/doi.org\/10.1136\/jcp.35.5.487","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,5]]}}}