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It is concluded that ELISA offers an economic and reliable alternative to isotope techniques for the assessment of antibody content in systemic lupus erythematosus (SLE) and related disease states for the clinical laboratory.<\/jats:p>","DOI":"10.1136\/jcp.35.5.566","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"566-573","source":"Crossref","is-referenced-by-count":28,"title":["A simple, rapid ELISA method for the detection of DNA antibodies."],"prefix":"10.1136","volume":"35","author":[{"given":"R P","family":"Stokes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Cordwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R A","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1982,5,1]]},"reference":[{"issue":"61","key":"ref_1","first-page":"7","article-title":"Antibodies to denatured deoxyribonucleic acid in lupus erythematosus serum. 1l-characterisation of antibodies in several sera","volume":"1962","author":"Stollar, D.B.; Levine, L.; Marmur, J.","unstructured":"Stollar DB, Levine L, Marmur J. 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Correlations between level. of DNA antibodies and clinical disease activity in SLEs Ann Rheum Dis 1977;36:157.","journal-title":"Ann Rheum Dis"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1002\/art.1780210402","article-title":"Analysis of DNA structure by Lydroxyapatite columns and ethidium bromide fluorescence techniques. A comparative study and effect on DNA binding","volume":"21","author":"Davis, P.; Burrington, M.; Russell, A.S.; Morgan, A.R.","year":"1978","unstructured":"Davis P, Burrington M, Russell AS, Morgan AR. Analysis of DNA structure by Lydroxyapatite columns and ethidium bromide fluorescence techniques. A comparative study and effect on DNA binding. 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Association of high avidity antinative DNA antibody with glomerulonephritis"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.35.5.566","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T09:39:15Z","timestamp":1765705155000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.35.5.566"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,5]]},"references-count":35,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1982,5,1]]},"published-print":{"date-parts":[[1982,5]]}},"alternative-id":["10.1136\/jcp.35.5.566"],"URL":"https:\/\/doi.org\/10.1136\/jcp.35.5.566","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1982,5]]}}}