{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:18:32Z","timestamp":1765729112910,"version":"3.48.0"},"reference-count":39,"publisher":"BMJ","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1983,12]]},"abstract":"<jats:p>Semiquantitative analysis of lactoferrin deficiency in neutrophil polymorphonuclear leucocytes in various haematological and non-haematological disease was carried out by scoring polymorphonuclear leucocytes stained for lactoferrin by the immunoperoxidase method. The staining patterns for lactoferrin were classified into four types (0-III) based on the intensity of reaction, and the sum of the ratings of 100 polymorphonuclear leucocytes was considered as \"lactoferrin score\" with a possible range of 0-300. As a result, significantly low lactoferrin-scores were frequently observed in acute leukaemias and the acute phase of chronic leukaemias. Of 35 cases with leukaemias, lactoferrin-negative polymorphonuclear leucocytes (type 0) were observed in the following cases: eight cases of acute myelogenous leukaemia (8\/14), a case of chronic myelogenous leukaemia (1\/10) in blast crisis, one of acute promyelocytic leukaemia (1\/1), one of acute monocytic leukaemia (1\/2), and a case of chronic myelomonocytic leukaemia (1\/2) in a transitional phase to an acute myelomonocytic leukaemia. In two cases of acute myelogenous leukaemia, in which the majority of polymorphonuclear leucocytes were negative for lactoferrin, ultrastructural cytochemical study revealed total lack of specific granules in these polymorphonuclear leucocytes. This suggests that lactoferrin is localised in the specific granules of neutrophils as has been postulated previously by others.<\/jats:p>","DOI":"10.1136\/jcp.36.12.1397","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"1397-1405","source":"Crossref","is-referenced-by-count":13,"title":["Lactoferrin-deficient neutrophil polymorphonuclear leucocytes in leukaemias: a semiquantitative and ultrastructural cytochemical study."],"prefix":"10.1136","volume":"36","author":[{"given":"J","family":"Miyauchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Enomoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Takeuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1983,12,1]]},"reference":[{"key":"ref_1","first-page":"115","article-title":"Studies on lactoferrin, the ironbinding protein of secretions","volume-title":"Prolides ofthe biological fluids","volume":"14","author":"Masson, P.L.; Heremans, J.F.","year":"1966","unstructured":"Masson PL, Heremans JF. Studies on lactoferrin, the ironbinding protein of secretions. In: Peeters H, ed. Prolides ofthe biological fluids. Amsterdam: Elsevier, 1966;14:115-24."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1126\/science.327545","article-title":"A bactericidal effect for human lactoferrin","author":"Arnold, R.R.; Cole, M.F.; McGhee, JR","year":"1977","unstructured":"Arnold RR, Cole MF, McGhee JR. A bactericidal effect for human lactoferrin. Science 1977;197:263-5.","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1136\/jcp.31.4.316","article-title":"Distribution of transferrin, ferritin, and lactoferrin in human tissues","volume":"31","author":"Mason, D.Y.; Taylor, C.R.","year":"1978","unstructured":"Mason DY, Taylor CR. Distribution of transferrin, ferritin, and lactoferrin in human tissues. J Clin Pathol 1978;31:316-27.","journal-title":"J Clin Pathol"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1084\/jem.130.3.643","article-title":"Lactoferrin, an ironbinding protein in neutrophilic leukocytes","volume":"130","author":"Masson, P.L.; Heremans, J.F.; Schonne, E.","year":"1969","unstructured":"Masson PL, Heremans JF, Schonne E. Lactoferrin, an ironbinding protein in neutrophilic leukocytes. J Exp Med 1969;130:643-58.","journal-title":"J Exp Med"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1084\/jem.131.3.559","article-title":"Association of lactoferrin with specific granules in rabbit heterophil leukocytes","volume":"131","author":"s Baggiolini M; C, DeDuve; PL, Masson; JF, Heremans","year":"1970","unstructured":"s Baggiolini M, DeDuve C, Masson PL, Heremans JF. Association of lactoferrin with specific granules in rabbit heterophil leukocytes. 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The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 1966;14:291-302.","journal-title":"J Histochem Cytochem"},{"key":"ref_16","first-page":"191","article-title":"Abnormal neutrophils in acute myelogenous leukemia: identification of subpopulations based on analysis of azurophil and specific granules","volume":"1","author":"Bainton, D.F.","year":"1975","unstructured":"Bainton DF. Abnormal neutrophils in acute myelogenous leukemia: identification of subpopulations based on analysis of azurophil and specific granules. 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Studies in developing neutrophils and monocytes from patients with myeloperoxidase deficienccy: comparison with peroxidase-deficient chicken heterophils. Lab Invest 1978;38:21-31.","journal-title":"Lab Invest"},{"issue":"9","key":"ref_22","first-page":"678","article-title":"Etude au microscope 6lectronique de la localisation des peroxydases dans les cellules de la moelle osseuse humaine","volume":"1969","author":"Breton-Gorius, J.; Guichard, J.","unstructured":"Breton-Gorius J, Guichard J. Etude au microscope 6lectronique de la localisation des peroxydases dans les cellules de la moelle osseuse humaine. 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The ultrastructural localization of human neutrophil alkaline phosphatase in normal individuals during pregnancy and patients with chronic granulocytic leukaemia. Histochem J 1981;13:31-43.","journal-title":"Histochem J"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1084\/jem.148.4.1052","article-title":"Identification of lactoferrin as the granulocyte-derived inhibitor of colony-stimulating activity production","volume":"148","author":"Broxmeyer, H.E.; Smithyman, A.; Eger, R.R.; Meyers, P.A.; Sousa, M.","year":"1978","unstructured":"Broxmeyer HE, Smithyman A, Eger RR, Meyers PA, Sousa M. Identification of lactoferrin as the granulocyte-derived inhibitor of colony-stimulating activity production. 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Histochem J 1978;10:31-43.","journal-title":"Histochem J"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1242\/jcs.36.1.401","article-title":"Studies on the subcellular localization of human neutrophil alkaline phosphatase","volume":"36","author":"Rustin, G.J.S.; Wilson, P.D.; Peters, T.J.","year":"1979","unstructured":"Rustin GJS, Wilson PD, Peters TJ. Studies on the subcellular localization of human neutrophil alkaline phosphatase. 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