{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:13:47Z","timestamp":1765728827307,"version":"3.48.0"},"reference-count":14,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1983,6]]},"abstract":"<jats:p>Operation specimens from 52 Hong Kong patients considered to have tuberculosis of the spine were sent at -78 degrees C to London where they were cultured on two L\u00f6wenstein-Jensen medium slopes, a slope of 7H11 medium made selective with antibiotics and in two bottles of selective liquid Kirchner medium. Cultures of M tuberculosis, usually with only scanty colonies, were obtained from 37 of the patients. Pus and caseous matter yielded more positive cultures and more numerous colonies than other specimens, but eight of the 37 patients yielded positive cultures only from tissues lining abscess walls or from bone or intervertebral disc specimens. Cultures in Kirchner medium were never contaminated and twice as many were positive as cultures on the slopes; nine patients had cultures positive only on Kirchner medium. It is recommended that (i) a variety of operation specimens, always including pus or caseous material in volumes of at least 1 ml, should be sent for bacteriology; (ii) specimens should be cultured in selective Kirchner medium, with or without L\u00f6wenstein-Jensen slopes; (iii) cultures should be incubated for a minimum of 8-9 wk.<\/jats:p>","DOI":"10.1136\/jcp.36.6.662","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"662-666","source":"Crossref","is-referenced-by-count":14,"title":["Examination of operation specimens from patients with spinal tuberculosis for tubercle bacilli."],"prefix":"10.1136","volume":"36","author":[{"given":"B W","family":"Allen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D A","family":"Mitchison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Darbyshire","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W W","family":"Chew","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Gabriel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1983,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1136\/jcp.34.4.404","article-title":"Extrapulmonary tuberculosis. 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Part VI The choice of culture media for the isolation of tubercle bacilli from sputum","volume":"17","author":"Public Health Laboratory Service","year":"1958","unstructured":"Public Health Laboratory Service. Cultivation of Mycobacterium tuberculosis. Part VI The choice of culture media for the isolation of tubercle bacilli from sputum. Mon Bul Min Health 1958;17: 112-8.","journal-title":"Mon Bul Min Health"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1099\/00222615-5-2-165","article-title":"A selective oleic acid albumin agar medium for tubercle bacilli","volume":"5","author":"Mitchison, D.A.; Allen, B.W.; Carrol, L.; Dickinson, J.M.; Aber, V.R.","year":"1972","unstructured":"Mitchison DA, Allen BW, Carrol L, Dickinson JM, Aber VR. A selective oleic acid albumin agar medium for tubercle bacilli. 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Prog Explor Tuberc 1966;15:125-88."},{"key":"ref_11","first-page":"255","article-title":"Detection of acid fast bacilli in material from cases of bone and joint tuberculosis","volume":"23","author":"Mathur, M.; Bhatia, V.N.; Agarwal, D.S.; Abbott, M.G.","year":"1980","unstructured":"Mathur M, Bhatia VN, Agarwal DS, Abbott MG. Detection of acid fast bacilli in material from cases of bone and joint tuberculosis. Ind J Pathol Microbiol 1980;23:255-60.","journal-title":"Ind J Pathol Microbiol"},{"key":"ref_12","first-page":"107","article-title":"Non tuberculous mycobacteria and associated diseases. State of the art","volume":"119","author":"Wolinsky, E.","year":"1979","unstructured":"Wolinsky E. Non tuberculous mycobacteria and associated diseases. State of the art. Am Rev Respir Dis 1979;119:107-59.","journal-title":"Am Rev Respir Dis"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0041-3879(72)90043-8","article-title":"Ending the routine guinea-pig test","volume":"53","author":"Marks, J.","year":"1972","unstructured":"Marks J. Ending the routine guinea-pig test. Tubercle 1972;53:31-4.","journal-title":"Tubercle"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1136\/jcp.26.4.250","article-title":"Selective media in the isolation of tubercle bacilli from tissues","volume":"26","author":"Mitchison, D.A.; Allen, B.W.; Lambert, R.A.","year":"1973","unstructured":"Mitchison DA, Allen BW, Lambert RA. Selective media in the isolation of tubercle bacilli from tissues. 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