{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T11:54:10Z","timestamp":1765713250054,"version":"3.48.0"},"reference-count":20,"publisher":"BMJ","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1983,8]]},"abstract":"<jats:p>A method for multimeric analysis of factor VIII related antigen\/von Willebrand protein is described. By modifying an existing method the technique has been simplified and optimised so that the different molecular forms of factor VIII related antigen and their triplet structure can be visualised. Clear differences can be seen between patterns in normals and type IIA von Willebrand's disease variants in that the latter lack high and intermediate multimers and also have a different configuration to the triplet pattern in the lower molecular weight multimers. Differences in triplet pattern can also be seen in different type IIA patients. The advantages and further application of this method for investigating the molecular defect in von Willebrand's disease are discussed.<\/jats:p>","DOI":"10.1136\/jcp.36.8.915","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"915-919","source":"Crossref","is-referenced-by-count":38,"title":["Analysis of the complexity of the multimeric structure of factor VIII related antigen\/von Willebrand protein using a modified electrophoretic technique."],"prefix":"10.1136","volume":"36","author":[{"given":"M S","family":"Enayat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F G","family":"Hill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1983,8,1]]},"reference":[{"key":"ref_1","first-page":"185","article-title":"Isolation and some chemical properties of human factor VIII (antihaemophilic factor)","volume":"77","author":"Hershgold, E.J.; Davison, A.M.; Janszen, M.E.","year":"1971","unstructured":"Hershgold EJ, Davison AM, Janszen ME. Isolation and some chemical properties of human factor VIII (antihaemophilic factor). J Lab Clin Med 1971;77:185-205.","journal-title":"J Lab Clin Med"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2151","DOI":"10.1172\/JCI107022","article-title":"Studies on the purification and characterization of human factor VIII","volume":"51","author":"Marchesi, S.L.; Shulman, N.R.; Gralnick, H.R.","year":"1972","unstructured":"Marchesi SL, Shulman NR, Gralnick HR. Studies on the purification and characterization of human factor VIII. J Clin Invest 1972;51:2151-61.","journal-title":"J Clin Invest"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3946","DOI":"10.1016\/S0021-9258(19)43824-6","article-title":"Isolation and characterization of human factor VIII (antihaemophilic factor)","volume":"248","author":"Legaz, M.E.; Schmer, G.; Counts, R.B.; Davie, E.W.","year":"1973","unstructured":"Legaz ME, Schmer G, Counts RB, Davie EW. 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Biochem Biophys Acta 1979;578:164-74.","journal-title":"Biochem Biophys Acta"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1182\/blood.V53.6.1095.1095","article-title":"Comparison of Factor VIII-related von Willebrand Factor proteins prepared from human cryoprecipitate and Factor VIII concentrate","volume":"53","author":"Wienstein, M.; Deykin, D.","year":"1979","unstructured":"Wienstein M, Deykin D. Comparison of Factor VIII-related von Willebrand Factor proteins prepared from human cryoprecipitate and Factor VIII concentrate. Blood 1979;53:1095-105.","journal-title":"Blood"},{"key":"ref_10","first-page":"590","article-title":"Multimetric structure of factor VIII\/von Willebrand factor in von Willebrand's disease","volume":"95","author":"Meyer, D.; Obert, B.; Pietu, G.; Lavergne, J.M.; Zimmerman, T.S.","year":"1980","unstructured":"Meyer D, Obert B, Pietu G, Lavergne JM, Zimmerman TS. Multimetric structure of factor VIII\/von Willebrand factor in von Willebrand's disease. J Lab Clin Med 1980;95:590-602.","journal-title":"J Lab Clin Med"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1056\/NEJM198005083021902","article-title":"Heightened interaction between platelets and Factor VIII\/ von Willebrand Factor in a new subtype of von Willebrand's disease","volume":"302","author":"Ruggeri, Z.M.; Fl, Pareti; Mannucci, P.M.; Ciavarella, N.; Zimmerman, T.S.","year":"1980","unstructured":"Ruggeri ZM, Pareti Fl, Mannucci PM, Ciavarella N, Zimmerman TS. Heightened interaction between platelets and Factor VIII\/ von Willebrand Factor in a new subtype of von Willebrand's disease. 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The iodination of follicle-stimulating and other hormones for radioimmunoassay. J Endocr 1972;55:453-4.","journal-title":"J Endocr"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1182\/blood.V59.6.1272.1272","article-title":"Multimeric Composition of Factor VIII\/von Willebrand Factor following administration of DDAVP: Implication for pathophysiology and therapy of von Willebrand's disease subtypes","volume":"59","author":"Ruggeri, Z.M.; Mannucci, P.M.; Lombardi, R.; Federici, A.B.; Zimmerman, T.S.","year":"1982","unstructured":"Ruggeri ZM, Mannucci PM, Lombardi R, Federici AB, Zimmerman TS. Multimeric Composition of Factor VIII\/von Willebrand Factor following administration of DDAVP: Implication for pathophysiology and therapy of von Willebrand's disease subtypes. 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