{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T08:10:22Z","timestamp":1765699822811,"version":"3.48.0"},"reference-count":12,"publisher":"BMJ","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1984,1]]},"abstract":"<jats:p>Six techniques were compared to find the most suitable method for determining the cytomegalovirus (CMV) antibody status of blood donors. Five hundred and ninety-six random sera were tested by immunofluorescence (IF), complement fixation (CFT), two enzyme linked immunosorbent assays (ELISA), a commercial indirect haemagglutination test (IHA)--used as supplied, and a locally devised micromodification of the same IHA test. Five hundred and thirty-five sera shared total agreement of results by all tests. The ELISA tests were the most discordant with other methods (10.5% discordancies both positive and negative). IF and CFT correlated well with other tests (0.8% discordances each) but for different reasons are unsatisfactory for donor screening. The IHA test used as supplied and its micromodification gave the most consistent results (0.8% and 0.5% discordancies respectively). The micromodification is easy to perform and read; it compares very favourably with CFT and IF for material costs and expertise required, and readily lends itself to the testing of large numbers of sera in a reasonable time. Within certain provisos the micro-IHA technique described is recommended as the most suitable test for blood donor screening.<\/jats:p>","DOI":"10.1136\/jcp.37.1.95","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"95-97","source":"Crossref","is-referenced-by-count":14,"title":["Comparative trial of six methods for the detection of CMV antibody in blood donors."],"prefix":"10.1136","volume":"37","author":[{"given":"A F","family":"Hunt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D L","family":"Allen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R L","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B A","family":"Robb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A Y","family":"Puckett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C C","family":"Entwistle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1984,1,1]]},"reference":[{"key":"ref_1","first-page":"53841","article-title":"Cytomegalovirus and blood transfusion in neonates","volume":"54","author":"Benson, J.W.T.; Bodden, S.J.; JO'H, Tobin","year":"1979","unstructured":"Benson JWT, Bodden SJ, Tobin JO'H. Cytomegalovirus and blood transfusion in neonates. Arch Dis Child 1979;54:53841.","journal-title":"Arch Dis Child"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0022-3476(81)80662-2","article-title":"Prevention of transfusion acquired cytomegalovirus infections in newborn infants","volume":"98","author":"Yeager, A.S.; Grumet, F.C.; Hafleigh, M.T.; Arvin, A.M.; Bradley, J.S.; Prober, C.G.","year":"1981","unstructured":"Yeager AS, Grumet FC, Hafleigh MT, Arvin AM, Bradley JS, Prober CG. Prevention of transfusion acquired cytomegalovirus infections in newborn infants. 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New York: Plenum Medical Books Co, 1982."},{"key":"ref_5","first-page":"13458","article-title":"Comparison of enzyme-linked immunoabsorbent assay, radioimmunoassay, complement fixation, anticomplement immunofluorescence and passive haemagglutination techniques for detecting cytomegalovirus IgG antibody","volume":"35","author":"Booth, J.C.; Hannington, B.; Bakir, T.M.F.","year":"1982","unstructured":"Booth JC, Hannington B, Bakir TMF, et al. Comparison of enzyme-linked immunoabsorbent assay, radioimmunoassay, complement fixation, anticomplement immunofluorescence and passive haemagglutination techniques for detecting cytomegalovirus IgG antibody. J Clin Pathol 1982;35:13458.","journal-title":"J Clin Pathol"},{"key":"ref_6","article-title":"In: Blood transfusion in clinical medicine","author":"Mollison, P.","year":"1983","unstructured":"Mollison P. In: Blood transfusion in clinical medicine 7th ed. 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