{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T17:40:01Z","timestamp":1758908401819,"version":"3.44.0"},"reference-count":18,"publisher":"BMJ","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1984,2]]},"abstract":"<jats:p>Factor VIII related antigen has been measured and epitope distribution has been explored by testing the degree of parallelism between standard and test plasma dose response curves using an enzyme immunoassay. Normal plasma, plasma fractions, and plasma from patients with haemophilia and von Willebrand's disease have been tested. All showed parallelism except for plasma from patients with the variant type IIA von Willebrand's disease, of which 10 had parallel and five had non-parallel dose response curves when compared with that of normal plasma. In one family plasma from seven members showed parallelism but from four others did not. An unrelated patient was tested on three occasions, and although the samples were parallel to each other, no sample was parallel to the standard. No correlation was found between parallelism as shown by the enzyme immunoassay and differences in factor VIII related antigen multimeric pattern, including triplet configuration, seen in the type IIA patients.<\/jats:p>","DOI":"10.1136\/jcp.37.2.194","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"194-199","source":"Crossref","is-referenced-by-count":1,"title":["Lack of correlation between factor VIII related antigen multimeric analysis pattern and parallel or non-parallel dose response curves in an ELISA factor VIII related antigen assay."],"prefix":"10.1136","volume":"37","author":[{"given":"P E","family":"Short","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C E","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M S","family":"Enayat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A M","family":"Picken","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F G","family":"Hill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1984,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1111\/j.1365-2141.1973.tb01778.x","article-title":"Factor VIII antigen and platelet retention in a glass bead column","volume":"25","author":"Bouma, B.N.; Sixma, J.J.; de Graff, S.; Wiegerinck, Y.; van Mourik, T.A.; Mochtar, I.A.","year":"1973","unstructured":"Bouma BN, Sixma JJ, de Graff S, Wiegerinck Y, van Mourik TA, Mochtar IA. Factor VIII antigen and platelet retention in a glass bead column. Br J Haematol 1973;25:645-56.","journal-title":"Br J Haematol"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1056\/NEJM197303222881202","article-title":"Two genetic variants of von Willebrand's disease","volume":"288","author":"Holmberg, L.; Nilsson, I.M.","year":"1973","unstructured":"Holmberg L, Nilsson IM. Two genetic variants of von Willebrand's disease. N Engl J Med 1973;288:595-8.","journal-title":"N Engl J Med"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1016\/S0140-6736(74)92652-X","article-title":"Evidence for a qualitative defect in factor VIII related antigen in von Willebrand's disease","volume":"i:","author":"Thomson, C.; Forbes, C.D.; Prentice, C.R.M.","year":"1974","unstructured":"Thomson C, Forbes CD, Prentice CRM. Evidence for a qualitative defect in factor VIII related antigen in von Willebrand's disease. Lancet 1974;i:594-6.","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1111\/j.1749-6632.1981.tb29733.x","article-title":"Classification of variant von Willebrand' s disease subtypes by analysis of functional characteristics and multimeric composition of factor VIII\/von Willebrand factor","volume":"370","author":"Ruggeri, Z.M.; Zimmerman, T.S.","year":"1981","unstructured":"Ruggeri ZM, Zimmerman TS. Classification of variant von Willebrand' s disease subtypes by analysis of functional characteristics and multimeric composition of factor VIII\/von Willebrand factor. Ann NY Acad Sci 1981;370:205-9.","journal-title":"Ann NY Acad Sci"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1111\/j.1365-2141.1974.tb00484.x","article-title":"A variant of factor VIII related antigen","volume":"26","author":"Kemoff, P.B.A.; Gruson, R.; Rizza, C.R.","year":"1974","unstructured":"Kemoff PBA, Gruson R, Rizza CR. A variant of factor VIII related antigen. Br J Haematol 1974;26:435-44.","journal-title":"Br J Haematol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1056\/NEJM197407182910301","article-title":"Inherited variants of factor VIII related protein in von Willebrand's disease","volume":"291","author":"Peake, I.R.; Bloom, A.L.; Giddings, J.C.","year":"1974","unstructured":"Peake IR, Bloom AL, Giddings JC. Inherited variants of factor VIII related protein in von Willebrand's disease. N Engl J Med 1974;291: 113-7.","journal-title":"N Engl J Med"},{"key":"ref_7","first-page":"276","article-title":"Von Willebrand' s syndrome. Studies on a variant factor VIII","volume":"5","author":"Hill, F.G.H.; Chan, M.C.K.; Hardisty, R.M.","year":"1976","unstructured":"Hill FGH, Chan MCK, Hardisty RM. Von Willebrand' s syndrome. Studies on a variant factor VIII. Haemostasis 1976;5:276-84.","journal-title":"Haemostasis"},{"key":"ref_8","first-page":"1318","article-title":"Variant von Wiliebrand's disease. Characterisations of two subtypes by analysis of multimeric composition of factor VIII\/von Willebrand factor in plasma and platelets","volume":"65","author":"ZM, K.Ruggeri; TS, Zimmerman","year":"1980","unstructured":"K Ruggeri ZM, Zimmerman TS. Variant von Wiliebrand's disease. Characterisations of two subtypes by analysis of multimeric composition of factor VIII\/von Willebrand factor in plasma and platelets. J Clin Invest 1980;65: 1318-25.","journal-title":"J Clin Invest"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1136\/jcp.36.8.915","article-title":"Analysis of the complexity of the multimeric structure of factor VIII related antigen\/von Willebrand protein using a modified electrophoretic technique","volume":"36","author":"Enayat, M.S.; Hill, F.G.H.","year":"1983","unstructured":"Enayat MS, Hill FGH. Analysis of the complexity of the multimeric structure of factor VIII related antigen\/von Willebrand protein using a modified electrophoretic technique. J Clin Pathol 1983;36:915-9.","journal-title":"J Clin Pathol"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1055\/s-0038-1649242","article-title":"Assay of FVIII related antigen in a variant of von Willebrand's disease","volume":"37","author":"Over, J.; Vlooswijk, H.A.A.; Sixma, J.J.","year":"1977","unstructured":"Over J, Vlooswijk HAA, Sixma JJ. Assay of FVIII related antigen in a variant of von Willebrand's disease. Thromb Haemostas 1977;37:367-70.","journal-title":"Thromb Haemostas"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0049-3848(77)90078-0","article-title":"The use of an immunoradiometric assay for factor VIII related antigen in the study of atypical von Willebrand's disease","volume":"10","author":"Peake, I.R.; Bloom, A.L.","year":"1977","unstructured":"Peake IR, Bloom AL. The use of an immunoradiometric assay for factor VIII related antigen in the study of atypical von Willebrand's disease. Thromb Res 1977;10:27-32.","journal-title":"Thromb Res"},{"key":"ref_12","first-page":"817","article-title":"Variants' of von Willebrand's disease. Demonstration of a decreased antigenic reactivity by immunoradiometric assay","volume":"i","author":"Ardaillou, N.; Girma, J.P.; Meyer, D.; Lavergne, J.M.; Shoa'i, I.; Larrieu, M.J.","year":"1978","unstructured":"Ardaillou N, Girma JP, Meyer D, Lavergne JM, Shoa'i I, Larrieu MJ. i Variants' of von Willebrand's disease. Demonstration of a decreased antigenic reactivity by immunoradiometric assay. Thromb Res 1978;5:817-30.","journal-title":"Thromb Res"},{"key":"ref_13","first-page":"351","article-title":"Abnormal antigenic reactivity of factor VIII\/von Willebrand factor subunit in variants of von Willebrand's disease","volume":"39","author":"Girma, J.P.; Pietu, G.; Lavergne, J.M.; Meyer, D.; Larrieu, M.J.","year":"1982","unstructured":"Girma JP, Pietu G, Lavergne JM, Meyer D, Larrieu MJ. Abnormal antigenic reactivity of factor VIII\/von Willebrand factor subunit in variants of von Willebrand's disease. J Lab Clin Med 1982;39:351-5. \" Short PE, Williams CE, Picken AM, Hill FGH. Factor VIII related antigen: an improved enzyme immunoassay. Med Lab Sci 1982;39:351-5.","journal-title":"J Lab Clin Med; 39: 351-5. \" Short PE, Williams CE, Picken AM, Hill FGH. Factor VIII related antigen: an improved enzyme immunoassay"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1055\/s-0038-1665298","article-title":"VIIIR: AG multimeric analysis of a large kindred with type IIA von Willebrand's disease","volume":"50","year":"1983","unstructured":"VIIIR:AG multimeric analysis of a large kindred with type IIA von Willebrand's disease. Thromb Haemostas 1983;50:735-9.","journal-title":"Thromb Haemostas"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/0049-3848(78)90148-2","article-title":"Preservation of platelet receptors for platelet aggregating factor by air drying, freezing or lyophilization: new stable platelet preparations for von Willebrand factor assays","volume":"13","author":"Brinkhous KM, Read MS","year":"1978","unstructured":"Brinkhous KM, Read MS. Preservation of platelet receptors for platelet aggregating factor by air drying, freezing or lyophilization: new stable platelet preparations for von Willebrand factor assays. Thromb Res 1978;13:591.","journal-title":"Thromb Res"},{"key":"ref_16","first-page":"357","article-title":"Qualitative VIIIR: AG function screening of multiple samples by two-dimensional crossed immunoelectrophoretic technique","volume":"39","author":"Enayat, M.S.; Hill, F.G.H.","year":"1982","unstructured":"Enayat MS, Hill FGH. Qualitative VIIIR:AG function screening of multiple samples by two-dimensional crossed immunoelectrophoretic technique. Med Lab Sci 1982;39:357-62.","journal-title":"Med Lab Sci"},{"key":"ref_17","first-page":"224","article-title":"Immunologic differentiation of classic haemophilia (factor VIII deficiency) and von Willebrand's disease with observations on combined deficiencies of anti-haemophilic factor and proaccelerin (factor V) and on an acquired circulating anticoagulant against haemophilic factor","volume":"50","author":"Zimmerman, T.S.; Ratnoff, O.D.; Pow6ll, A.E.","year":"1971","unstructured":"Zimmerman TS, Ratnoff OD, Pow6ll AE. Immunologic differentiation of classic haemophilia (factor VIII deficiency) and von Willebrand's disease with observations on combined deficiencies of anti-haemophilic factor and proaccelerin (factor V) and on an acquired circulating anticoagulant against haemophilic factor. J Clin Invest 1971;50:224-54.","journal-title":"J Clin Invest"},{"key":"ref_18","article-title":"Statistical methods in medical research","author":"Armitage, P.","year":"1971","unstructured":"Armitage P. Statistical methods in medical research. London: Blackwell Scientific Publications, 1971."}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.37.2.194","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T17:01:58Z","timestamp":1758906118000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.37.2.194"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,2]]},"references-count":18,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1984,2,1]]},"published-print":{"date-parts":[[1984,2]]}},"alternative-id":["10.1136\/jcp.37.2.194"],"URL":"https:\/\/doi.org\/10.1136\/jcp.37.2.194","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1984,2]]}}}