{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T11:34:45Z","timestamp":1765712085257,"version":"3.48.0"},"reference-count":29,"publisher":"BMJ","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1985,1]]},"abstract":"<jats:p>We present the data obtained from routine quantitation of normal venous blood mononuclear cells using 19 monoclonal antibodies against defined mononuclear cell surface antigens. The results indicate different values for percentage and absolute numbers of T lymphocytes and of T lymphocyte subsets depending on the monoclonal antibodies used to quantify these populations. In most instances the total number of identified cells was significantly less than the total number of recovered blood mononuclear cells, which suggests the existence in blood of a null cell population. Evidence is advanced to support previous observations that at least a proportion of this population is of B cell lineage, expressing cytoplasmic immunoglobulin but lacking other class or lineage specific markers. The value of a diverse monoclonal panel in routine quantitation of peripheral blood mononuclear cells is discussed.<\/jats:p>","DOI":"10.1136\/jcp.38.1.54","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"54-59","source":"Crossref","is-referenced-by-count":12,"title":["Normal values for the different classes of venous blood mononuclear cells defined by monoclonal antibodies."],"prefix":"10.1136","volume":"38","author":[{"given":"L K","family":"Goff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Habeshaw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M L","family":"Rose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Gracie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Gregory","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1985,1,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.4049\/jimmunol.123.3.1312","article-title":"A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human cells","volume":"123","author":"Reinherz, E.L.; Kung, P.C.; Goldstein, G.; Schlossman, S.F.","year":"1979","unstructured":"Reinherz EL, Kung PC, Goldstein G, Schlossman SF. A monoclonal antibody with selective reactivity with functionally mature human thymocytes and all peripheral human cells. J Immunology 1979; 123:1312-7.","journal-title":"J Immunology"},{"key":"ref_2","first-page":"544","article-title":"Thymus dependent membrane antigens in man: inhibition of cell mediated lympholysis by monoclonal antibodies","volume-title":"to TH2 antigen. Proc Natl Acad Sci","volume":"78","author":"Evans, R.L.; Wall, D.W.; Platsoucas, C.D.","year":"1981","unstructured":"Evans RL, Wall DW, Platsoucas CD, et al. Thymus dependent membrane antigens in man: inhibition of cell mediated lympholysis by monoclonal antibodies to TH2 antigen. 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Distinctive functional characteristics of human T' lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol 1981; 11:329-34.","journal-title":"Eur J Immunol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.4049\/jimmunol.124.3.1301","article-title":"A monoclonal antibody reactive with the human cytotoxic\/suppressor T cell subset previously defined by a heteroantiserum termed TH2","volume":"124","author":"Reinherz, E.L.; Kung, P.C.; Goldstein, G.; Schlossman, S.F.","year":"1980","unstructured":"Reinherz EL, Kung PC, Goldstein G, Schlossman SF. A monoclonal antibody reactive with the human cytotoxic\/suppressor T cell subset previously defined by a heteroantiserum termed TH2. J Immunol 1980; 124:1301-7.","journal-title":"J Immunol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1002\/eji.1830120115","article-title":"Monoclonal antibodies OKT1 1 and OKT1 1A have pan-T reactivity and block sheep erythrocyte \" receptors\"","volume":"12","author":"Verbi, W.; Greaves, M.F.; Schneider, C.","year":"1982","unstructured":"Verbi W, Greaves MF, Schneider C, et al. Monoclonal antibodies OKT1 1 and OKT1 1A have pan-T reactivity and block sheep erythrocyte \" receptors\". Eur J Immunol 1982; 12:81-6.","journal-title":"Eur J Immunol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3848","DOI":"10.1073\/pnas.78.6.3848","article-title":"Expression of cell surface markers after human B lymphocyte activation","volume":"78","author":"Stashenko, P.; Nadler, L.M.; Hardy, R.; Schlossman, S.F.","year":"1981","unstructured":"Stashenko P, Nadler LM, Hardy R, Schlossman SF. Expression of cell surface markers after human B lymphocyte activation. 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A monoclonal antibody with reactivity for lymphoid cells transformed or activated by EBV. J Cell Biochem 1982;6:39.","journal-title":"J Cell Biochem"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/283583a0","article-title":"A monoclonal antibody to human acute lymphoblastic leukaemia antigen","volume":"283","author":"Ritz, J.; Pesando, J.M.; Notis-McConarty, J.; Lazarus, H.; Schlossman, S.F.","year":"1980","unstructured":"Ritz J, Pesando JM, Notis-McConarty J, Lazarus H, Schlossman SF. A monoclonal antibody to human acute lymphoblastic leukaemia antigen. Nature 1980;283:583-5.","journal-title":"Nature"},{"key":"ref_13","first-page":"1024","article-title":"A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1). J1mmunol","volume":"127","author":"Abo, T.; Balch, C.M.","year":"1981","unstructured":"Abo T, Balch CM. A differentiation antigen of human NK and K cells identified by a monoclonal antibody (HNK-1).J1mmunol 1981; 127:1024-9."},{"key":"ref_14","first-page":"342","article-title":"Use of a monoclonal antibody (W6\/32) in structural studies of HLA-A","volume":"123","author":"Parham, P.; Barnstable, C.J.; Bodmer, W.F.","year":"1979","unstructured":"Parham P, Barnstable CJ, Bodmer WF. Use of a monoclonal antibody (W6\/32) in structural studies of HLA-A, B, C antigens. J Immunol 1979; 123:342-9.","journal-title":"B, C antigens. J Immunol"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1038\/287332a0","article-title":"Isolation of human haematopoietic or progenitor cells using monoclonal antibodies","volume":"287","author":"Beverley, P.C.L.; Linch, D.; Delia, D.","year":"1980","unstructured":"Beverley PCL, Linch D, Delia D. Isolation of human haematopoietic or progenitor cells using monoclonal antibodies. 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Phenotypes of \"null\" lymphoid cells in human blood. 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