{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:41:34Z","timestamp":1761100894564,"version":"3.44.0"},"reference-count":26,"publisher":"BMJ","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1985,11]]},"abstract":"<jats:p>Tissue sections embedded in paraffin and fixed in formalin from 32 patients with Hodgkin's disease, representing the major histological subtypes, were studied using two granulocyte specific monoclonal antibodies (Leu-M1 and 3C4) and an HLA-D region specific monoclonal antibody (TAL-IB5). Reed-Sternberg cells were stained with one or other of the antigranulocyte antibodies in the nodular sclerosing and lymphocyte depleted subtypes. Reed-Sternberg cells in all but three cases of mixed cellularity Hodgkin's disease were positive with both Leu-M1 and 3C4. One case stained with only Leu-M1, and two cases were consistently negative with both antibodies. HLA-DR was widely expressed in the Reed-Sternberg cells of all three subtypes. In the four cases of lymphocyte predominant Hodgkin's disease the multinucleated Reed-Sternberg cells did not stain with either antigranulocyte antibody but were strongly positive with anti-HLA-DR. Twenty five cases of non-Hodgkin's lymphoma, in which there were multinucleated giant cells resembling Reed-Sternberg cells, were studied in a similar way. These cases included pleomorphic T cell and B cell lymphomas, histiocytic lymphomas, and malignant histiocytosis of the intestine. In none of these did the multinucleated cells stain with either antigranulocyte antibody, but in most cases the multinucleated cells stained with anti-HLA-DR. In two cases of the tumour stage of mycosis fungoides dot like intracytoplasmic staining was shown in the tumour cells with both antigranulocyte markers. The monoclonal antigranulocyte antibodies Leu-M1 and 3C4 are of considerable value in both the diagnosis and the differential diagnosis of Hodgkin's disease and are particularly valuable in that they can be applied to tissue fixed in formalin and embedded in paraffin. Antibody to HLA-DR, while useful, is of less value.<\/jats:p>","DOI":"10.1136\/jcp.38.11.1241","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"1241-1246","source":"Crossref","is-referenced-by-count":33,"title":["Granulocyte and HLA-D region specific monoclonal antibodies in the diagnosis of Hodgkin's disease."],"prefix":"10.1136","volume":"38","author":[{"given":"A J","family":"Norton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P G","family":"Isaacson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1985,11,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1016\/S0140-6736(69)90552-2","article-title":"Reed-Sternberg-like cells in infectious mononucleosis","volume":"ii:","author":"Lukes, R.J.; Tindle, B.H.; Parker, J.W.","year":"1969","unstructured":"Lukes RJ, Tindle BH, Parker JW. Reed-Sternberg-like cells in infectious mononucleosis. Lancet 1969;ii: 1003-4.","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1093\/ajcp\/58.6.607","article-title":"\"Reed-Sternberg' cells in infectious mononucleosis?","volume":"58","author":"Tindle, B.H.; Parker, J.W.; Lukes, R.J.","year":"1972","unstructured":"Tindle BH, Parker JW, Lukes RJ. \"Reed-Sternberg' cells in infectious mononucleosis? Am J Clin Pathol 1972;58: 607-17.","journal-title":"Am J Clin Pathol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1002\/1097-0142(197007)26:1<176::AID-CNCR2820260123>3.0.CO;2-I","article-title":"Observation of cells resembling Sternberg-Reed cells in conditions other than Hodgkin's disease","volume":"26","author":"Strum, S.B.; Park, J.K.; Rappaport, H.","year":"1970","unstructured":"Strum SB, Park JK, Rappaport H. Observation of cells resembling Sternberg-Reed cells in conditions other than Hodgkin's disease. Cancer 1970;26: 176-90.","journal-title":"Cancer"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1093\/ajcp\/72.4.540","article-title":"Malignant lymphoma, T-cell type. A distinct morphologic variant with large multilobulated nuclei, with a report of four cases","volume":"72","author":"Pinkus, G.S.; Said, J.W.; Hargreaves, H.","year":"1979","unstructured":"Pinkus GS, Said JW, Hargreaves H. Malignant lymphoma, T-cell type. A distinct morphologic variant with large multilobulated nuclei, with a report of four cases. Am J Clin Pathol 1979;72:540-50.","journal-title":"Am J Clin Pathol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1093\/ajcp\/76.2.190","article-title":"Non-Hodgkin's lymphoma of large multilobulated cell type. A clinicopathologic study of ten cases","volume":"76","author":"Weinberg, D.S.; Pinkus, G.S.","year":"1981","unstructured":"Weinberg DS, Pinkus GS. Non-Hodgkin's lymphoma of large multilobulated cell type. A clinicopathologic study of ten cases. Am J Clin Pathol 1981; 76: 190-6.","journal-title":"Am J Clin Pathol"},{"key":"ref_6","first-page":"106","article-title":"Immunohistological analysis of human lymphoma: correlation of histological and immunological categories","volume-title":"Advances in cancer research","author":"Stein, H.; Lennert, K.; Feller, A.C.; Mason, D.Y.","year":"1984","unstructured":"Stein H, Lennert K, Feller AC, Mason DY. Immunohistological analysis of human lymphoma: correlation of histological and immunological categories. In: Klein G, Weinhouse S, eds. Advances in cancer research. New York: Academic Press, 1984:106."},{"key":"ref_7","first-page":"278","article-title":"Biopsy pathology ofthe lymphoreticular system","author":"Wright, D.H.; Isaacson, P.G.","year":"1983","unstructured":"Wright DH, Isaacson PG. Biopsy pathology ofthe lymphoreticular system. 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A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopathol 1982; 23:172-88.","journal-title":"Clin Immunol Immunopathol"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1182\/blood.V63.6.1299.1299","article-title":"A Hodgkin cellspecific antigen is expressed on a subset of autoand alloactivated T (helper) lymphoblasts","volume":"63","author":"Andreesen, R.; Osterholz, J.; Lohr, G.; Bross, K.","year":"1984","unstructured":"Andreesen R, Osterholz J, Lohr G, Bross K. A Hodgkin cellspecific antigen is expressed on a subset of autoand alloactivated T (helper) lymphoblasts. Blood 1984;63: 1299-302.","journal-title":"Blood"},{"article-title":"The expression of the Hodgkin's disease associated antigen Ki-1 in reactive and neoplastic lymphoid tissue. Evidence that Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells. Blood","author":"Stein, H.; Mason, D.Y.; Gerdes, J.","key":"ref_25","unstructured":"Stein H, Mason DY, Gerdes J, et al. The expression of the Hodgkin's disease associated antigen Ki-1 in reactive and neoplastic lymphoid tissue. Evidence that Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells. Blood. (in press)."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S0140-6736(85)91298-X","article-title":"Leu-Ml monoclonal antibody staining of Reed-Sternberg cells","volume":"i:","author":"Schnitzer, B.; Hyder, D.","year":"1985","unstructured":"Schnitzer B, Hyder D. Leu-Ml monoclonal antibody staining of Reed-Sternberg cells. Lancet 1985;i: 757.","journal-title":"Lancet"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.38.11.1241","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T12:15:40Z","timestamp":1758802540000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.38.11.1241"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,11]]},"references-count":26,"journal-issue":{"issue":"11","published-online":{"date-parts":[[1985,11,1]]},"published-print":{"date-parts":[[1985,11]]}},"alternative-id":["10.1136\/jcp.38.11.1241"],"URL":"https:\/\/doi.org\/10.1136\/jcp.38.11.1241","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1985,11]]}}}