{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T12:48:09Z","timestamp":1758804489014,"version":"3.44.0"},"reference-count":8,"publisher":"BMJ","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:p>A lysis filtration system was used in conjunction with conventional broth culture for 1112 blood cultures. The system, which entailed collection of 5 ml of blood into bottles containing 50 ml isotonic phosphate buffer, Tween 20, and Rhozyme with subsequent filtration using a 0.45 micron Millipore field monitor, was simple and economical to use. Positive results were obtained earlier than those obtained with conventional broth cultures, and almost twice as many fungi and yeasts were isolated. Some fastidious organisms such as Haemophilus influenzae and Streptococcus pneumoniae however, were not recovered from the lysis system, and contaminants in lysis cultures were three times as common as in conventional culture. The number of positive cultures was also adversely influenced by incubation of the blood lysis mixture overnight before filtration. We conclude that this lysis filtration system is useful as an adjunct to conventional broth culture in selected patients in cases in which filtration can be carried out soon after collection.<\/jats:p>","DOI":"10.1136\/jcp.39.1.89","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"89-92","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of lysis filtration as an adjunct to conventional blood culture."],"prefix":"10.1136","volume":"39","author":[{"given":"R","family":"Chan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Munro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Tomlinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1986,1,1]]},"reference":[{"key":"ref_1","first-page":"154","article-title":"Improved method for cultivation of Brucella","volume-title":"from the blood. Proc Soc Exp Biol Med","volume":"85","author":"Braun, W.; Kelsh, J.","year":"1954","unstructured":"Braun W, Kelsh J. Improved method for cultivation of Brucella from the blood. Proc Soc Exp Biol Med 1954;85:154-5."},{"key":"ref_2","first-page":"561","volume-title":"Proc Soc Exp Biol Med 1955","volume":"88","unstructured":"Proc Soc Exp Biol Med 1955;88:561-3."},{"key":"ref_3","first-page":"539","article-title":"Rapid diagnosis of bacteraemia with quantitative differentialmembrane filtration culture","author":"Winn, W.R.; White, M.L.; Carter, W.T.; Miller, A.B.; Finegold, S.M.","year":"1966","unstructured":"Winn WR, White ML, Carter WT, Miller AB, Finegold SM. Rapid diagnosis of bacteraemia with quantitative differentialmembrane filtration culture. JAMA 1966;197:539-48.","journal-title":"JAMA"},{"key":"ref_4","first-page":"105","article-title":"Rapid diagnosis of bacteraemia. Appl Microbiol 1969; 18: 458-63. sKozub WR, Kirkham WR, Chatman CE, Pribor HC. A practical blood culturing method employing dilution and filtration","volume":"52","author":"Finegold, S.M.; White, M.L.; l, Ziement; Winn, W.R.","year":"1969","unstructured":"Finegold SM, White ML, Ziement l, Winn WR. Rapid diagnosis of bacteraemia. Appl Microbiol 1969;18:458-63. sKozub WR, Kirkham WR, Chatman CE, Pribor HC. A practical blood culturing method employing dilution and filtration. Am J Clin Pathol 1969;52:105-13.","journal-title":"Am J Clin Pathol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1128\/AEM.23.3.500-504.1972","article-title":"Evaluation of the Sterifil lysisfiltration blood culture system","volume":"23","author":"Farmer, S.G.; Komorowski, R.A.","year":"1972","unstructured":"Farmer SG, Komorowski RA. Evaluation of the Sterifil lysisfiltration blood culture system. Appl Microbiol 1972;23:500-4.","journal-title":"Appl Microbiol"},{"key":"ref_6","first-page":"172","volume":"15","author":"Microbiol, J.Clin","year":"1982","unstructured":"J Clin Microbiol 1982;15: 172-4."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1128\/JCM.20.5.927-932.1984","article-title":"Comparison of lysis-centrifugation with lysis filtration and conventional unvented bottles for blood cultures","volume":"20","year":"1984","unstructured":"Comparison of lysis-centrifugation with lysis filtration and conventional unvented bottles for blood cultures. J Clin Microbiol 1984;20:927-32.","journal-title":"J Clin Microbiol"},{"key":"ref_8","article-title":"Manual for the identification of medical bacteria","author":"Cowan, S.T.; Steel, K.J.","year":"1974","unstructured":"Cowan ST, Steel KJ. Manual for the identification of medical bacteria. 2nd ed. Cambridge: Cambridge University Press, 1974.","edition":"2nd ed"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.39.1.89","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T12:00:02Z","timestamp":1758801602000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.39.1.89"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,1]]},"references-count":8,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1986,1,1]]},"published-print":{"date-parts":[[1986,1]]}},"alternative-id":["10.1136\/jcp.39.1.89"],"URL":"https:\/\/doi.org\/10.1136\/jcp.39.1.89","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1986,1]]}}}