{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T04:40:32Z","timestamp":1765082432769,"version":"3.46.0"},"reference-count":15,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1986,4]]},"abstract":"<jats:p>UCHL1 is a murine monoclonal antibody that recognises a 180-185 kD determinant on CD4 (72%) and CD8 (36%) positive T cells. This antibody is effective in formalin fixed and paraffin embedded tissues, using the immunoperoxidase method. One hundred and forty three cases of malignant lymphoma were examined. Neoplastic cells in 100% of cases of Mycosis fungoides (n = 10), 83% of cases of peripheral T cell lymphoma (n = 25), and 78% of cases of (T-ALL) T acute lymphoblastic lymphoma (n = 9) were stained by this antibody. In addition, staining was seen in 100% of cases of malignant histiocytosis of the intestine (n = 13), a condition now thought to be a T cell lymphoma. Two cases of true histiocytic lymphoma were also positive. This antibody stained neither the neoplastic cells in a wide range of B cell lymphomas (n = 62) nor Reed-Sternberg cells in 16 cases of Hodgkin's disease. UCHL1 also stained neoplastic cells in four cases of granulocytic sarcoma. A panel of normal tissues was similarly studied. Staining was seen in normal T cells and mucosal intraepithelial lymphocytes, macrophages, mature myeloid cells, and endometrial stromal granulocytes. UCHL1 is a monoclonal antibody that identifies T cells in formalin fixed paraffin embedded tissues, and should prove useful for diagnosing T cell lymphomas, especially when only formalin fixed tissue is available for diagnosis.<\/jats:p>","DOI":"10.1136\/jcp.39.4.399","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"399-405","source":"Crossref","is-referenced-by-count":197,"title":["Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues."],"prefix":"10.1136","volume":"39","author":[{"given":"A J","family":"Norton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A D","family":"Ramsay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S H","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P C","family":"Beverley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P G","family":"Isaacson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1986,4,1]]},"reference":[{"article-title":"Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHLI","author":"Smith, S.H.; Brown, M.H.; Rowe, D.","key":"ref_1","unstructured":"Smith SH, Brown MH, Rowe D, et al. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHLI. Immunology (in press)."},{"key":"ref_2","article-title":"Biopsy pathology of the lymphoreticular system","author":"Wright, D.H.; Isaacson, P.G.","year":"1983","unstructured":"Wright DH, Isaacson PG. Biopsy pathology of the lymphoreticular system. London: Chapman and Hall, 1983."},{"key":"ref_3","first-page":"249","article-title":"Immunocytochemistry of lymphoreticular tumours","volume-title":"Immunocytochemistry. Practical applications in pathology and biology","author":"Isaacson, P.G.; Wright, D.H.","year":"1983","unstructured":"Isaacson PG, Wright DH. Immunocytochemistry of lymphoreticular tumours. In: Polak JM, Van Noorden S, eds. Immunocytochemistry. Practical applications in pathology and biology. 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