{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T13:32:18Z","timestamp":1776691938876,"version":"3.51.2"},"reference-count":8,"publisher":"BMJ","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1987,11]]},"abstract":"<jats:p>The alkaline phosphatase-antialkaline phosphatase (APAAP) immunocytochemical staining technique was used to look for circulating cells of megakaryocyte lineage in peripheral blood smears from 67 cases of myelodysplasia. Small numbers of micromegakaryocytes positive for platelet glycoprotein IIIa were found in 23 cases. These cells superficially resemble small lymphoid cells and are hence difficult or impossible to recognise in conventional Romanowsky stained smears. Circulating micromegakaryocytes were found most commonly in more aggressive types of myelodysplasia (such as refractory anaemia with excess blasts (RAEB) and refractory anaemia with excess blasts in transformation (RAEB-t], and their presence may therefore indicate a poor prognosis. Because of the simplicity of this immunocytochemical labelling technique, it could be of wide use in the initial assessment of patients with myelodysplasia, and possibly for the early detection of acute leukaemic transformation.<\/jats:p>","DOI":"10.1136\/jcp.40.11.1349","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"1349-1352","source":"Crossref","is-referenced-by-count":29,"title":["Circulating micromegakaryocytes in myelodysplasia."],"prefix":"10.1136","volume":"40","author":[{"given":"W N","family":"Erber","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Jacobs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D G","family":"Oscier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A M","family":"O'Hea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D Y","family":"Mason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1987,11,1]]},"reference":[{"key":"ref_1","first-page":"83","article-title":"Myelodysplastic syndromes: a study of 101 cases according to the FAB classification","volume":"61","author":"Vallespi, T.; Torrabadella, A.; Julia, D.","year":"1985","unstructured":"Vallespi T, Torrabadella A, Julia D, et al. Myelodysplastic syndromes: a study of 101 cases according to the FAB classification. Br J Haematol 1985;61:83-92. coprotein seen in myelodysplasia are similar in morphology to the micromegakaryocytes we have previously detected (also by means of the APAAP staining technique) in the circulation of some cases of","journal-title":"Br J Haematol; coprotein seen in myelodysplasia are similar in morphology to the micromegakaryocytes we have previously detected (also by means of the APAAP staining technique) in the circulation of some cases of"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.1365-2141.1982.tb08475.x","article-title":"Proposals for the classification of the myelodysplastic syndromes","volume":"51","author":"Bennett, J.M.; Catovsky, D.; Daniel, M.T.","year":"1982","unstructured":"Bennett JM, Catovsky D, Daniel MT, et al. Proposals for the classification of the myelodysplastic syndromes. Br J Haematol 1982;51:189-99.","journal-title":"Br J Haematol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1111\/j.1365-2141.1987.tb06140.x","article-title":"Detection of cells of megakaryocyte lineage in acute myeloblastic leukaemia4 and other myeloproliferative disorders (such as chronic granulocytic leukaemia and essential thrombocythemia; unpublished observations). The present study sheds haematologic malignancies by immuno-alkaline phosphatase labelling cell smears with a panel of monoclonal antibodies","volume":"65","author":"Erber, W.N.; Breton-Gorius, J.; Villeval, J.L.; Oscier, D.G.; Bai, Y.; Mason, D.Y.","year":"1987","unstructured":"Erber WN, Breton-Gorius J, Villeval JL, Oscier DG, Bai Y, Mason DY. Detection of cells of megakaryocyte lineage in acute myeloblastic leukaemia4 and other myeloproliferative disorders (such as chronic granulocytic leukaemia and essential thrombocythemia; unpublished observations). The present study sheds haematologic malignancies by immuno-alkaline phosphatase labelling cell smears with a panel of monoclonal antibodies. Br J Haematol 1987;65:87-94.","journal-title":"Br J Haematol"},{"key":"ref_4","first-page":"509","article-title":"Von Dem Borne AEG. Monoclonal antibodies against human platelet glylight on the origin of the circulating micromegakaryocytes seen in patients with myelodysplasia as immunocytochemical labelling of bone marrow smears revealed, in addition to the previously coprotein II1a","volume":"55","author":"Tetteroo, P.A.T.; Lansdorp, P.M.; Leeksma, O.C.","year":"1983","unstructured":"Tetteroo PAT, Lansdorp PM, Leeksma OC, Von Dem Borne AEG. Monoclonal antibodies against human platelet glylight on the origin of the circulating micromegakaryocytes seen in patients with myelodysplasia as immunocytochemical labelling of bone marrow smears revealed, in addition to the previously coprotein II1a. Br J Haematol 1983;55:509-22.","journal-title":"Br J Haematol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1177\/32.2.6198355","article-title":"Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP) complexes","volume":"32","author":"Cordell, J.L.; Falini, B.; Erber, W.N.","year":"1984","unstructured":"Cordell JL, Falini B, Erber WN, et al. Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP) complexes. J Histochem Cytochem 1984;32:219-29.","journal-title":"J Histochem Cytochem"},{"key":"ref_6","first-page":"145","article-title":"Immunoenzymatic labelling of haematological samples with monoclonal antibodies","volume-title":"Methods in haematology: monoclonal antibodies","author":"Mason, D.Y.; Erber, W.N.; Falini, B.; Stein, H.; Gatter, K.C.","year":"1986","unstructured":"Mason DY, Erber WN, Falini B, Stein H, Gatter KC. Immunoenzymatic labelling of haematological samples with monoclonal antibodies. In: Beverley PCL, ed. Methods in haematology: monoclonal antibodies. Edinburgh: Churchill Livingstone, 1986:145-81. megakaryocytes were most commonly detected in cases of the RAEB and RAEB-t FAB subtypes of myelodysplastic syndrome (table). It has previously been noted that these two diagnostic groups are most"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1111\/j.1365-2141.1986.tb07550.x","article-title":"Diagnostic significance ofdetecting pseudo-Pelger-Huet anomalies and micro-megakaryocytes in myelodysplastic syndrome","volume":"63","author":"Kuriyama, K.; Tomonaga, M.; Matsuo, T.; Ginnai, I.; Ichimaru, M.","year":"1986","unstructured":"Kuriyama K, Tomonaga M, Matsuo T, Ginnai I, Ichimaru M. Diagnostic significance ofdetecting pseudo-Pelger-Huet anomalies and micro-megakaryocytes in myelodysplastic syndrome. Br J Haematol 1986;63:665-9.","journal-title":"Br J Haematol"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1136\/jcp.36.10.1129","article-title":"often associated with dysmegakaryopoietic features Haematological features of primary myclodysplastic synin the marrow9 and transformation to acute leukaemia.2 1 The \"spill-over\" of micromegakaryocytes dromes (PMDS) at initial presentation: a study of 118 cases","volume":"36","author":"Juneja, S.K.; Imbert, M.; Jouault, H.; Scoazec, J.Y.; Sigaux, F.; Sultan, C.","year":"1983","unstructured":"Juneja SK, Imbert M, Jouault H, Scoazec JY, Sigaux F, Sultan C. often associated with dysmegakaryopoietic features Haematological features of primary myclodysplastic synin the marrow9 and transformation to acute leukaemia.2 1 The \"spill-over\" of micromegakaryocytes dromes (PMDS) at initial presentation: a study of 118 cases. J Clin Pathol 1983;36:1129-35.","journal-title":"J Clin Pathol"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.40.11.1349","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T13:28:58Z","timestamp":1758806938000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.40.11.1349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,11]]},"references-count":8,"journal-issue":{"issue":"11","published-online":{"date-parts":[[1987,11,1]]},"published-print":{"date-parts":[[1987,11]]}},"alternative-id":["10.1136\/jcp.40.11.1349"],"URL":"https:\/\/doi.org\/10.1136\/jcp.40.11.1349","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,11]]}}}