{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:26:51Z","timestamp":1770823611527,"version":"3.50.1"},"reference-count":35,"publisher":"BMJ","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1987,2]]},"abstract":"<jats:p>A total of 67 samples from normal and pathological thyroid glands were stained (as formalin fixed paraffin sections) with a panel of monoclonal antibodies directed against intermediate filament proteins. The study confirmed previous reports of cytokeratin and vimentin coexpression in primary thyroid carcinomas, but coexpression was also detected in normal thyroid and in a range of benign conditions including follicular adenomas, Hashimoto's thyroiditis, and diffuse hyperplasia (thyrotoxicosis). Prekeratin expression was found (using antibodies recognising higher molecular weight cytokeratins) predominantly in areas of squamous change, independent of the underlying thyroid pathology. This study does not therefore support previous findings that prekeratin expression provides a reliable means of distinguishing follicular pattern papillary carcinoma from follicular carcinoma with its poorer prognosis or that it helps distinguish benign from malignant papillary lesions. No evidence of desmin or neurofilament expression was seen, and in particular, neurofilaments could not be detected in any of the cases of medullary carcinoma studied.<\/jats:p>","DOI":"10.1136\/jcp.40.2.136","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"136-142","source":"Crossref","is-referenced-by-count":35,"title":["Expression of intermediate filament proteins in normal and diseased thyroid glands."],"prefix":"10.1136","volume":"40","author":[{"given":"I D","family":"Buley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K C","family":"Gatter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Heryet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D Y","family":"Mason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1987,2,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.3109\/01913128409141467","article-title":"Antibodies to intermediate filament proteins in the diagnosis and classification of human tumours","volume":"7","author":"Miettinen, M.; Lehto, V.-P.; Virtanen, I.","year":"1984","unstructured":"Miettinen M, Lehto V-P, Virtanen I. Antibodies to intermediate filament proteins in the diagnosis and classification of human tumours. Ultrastruct Pathol 1984;7:83-107.","journal-title":"Ultrastruct Pathol"},{"key":"ref_2","first-page":"262","article-title":"Expression of intermediate filament proteins in thyroid gland and thyroid tumours","volume":"50","author":"Miettinen, M.; Franssila, K.; Lehto, V.-P.; Paasivuo, R.; Virtanen, I.","year":"1984","unstructured":"Miettinen M, Franssila K, Lehto V-P, Paasivuo R, Virtanen I. Expression of intermediate filament proteins in thyroid gland and thyroid tumours. Lab Invest 1984;50:262-70.","journal-title":"Lab Invest"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/BF00618871","article-title":"Immunohistochemical analysis of thyroglobulin and keratin in benign and malignant thyroid tumours","volume":"398","author":"Permanetter, W.; Nathrath, W.B.J.; Lohrs, V.","year":"1982","unstructured":"Permanetter W, Nathrath WBJ, Lohrs V. 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Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations. Lab Invest 1985;52:3:243-56.","journal-title":"Lab Invest"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/0092-8674(82)90400-7","article-title":"The catalogue of human cytokeratins: patterns of expression in normal epithelia, tumours and cultured cells","volume":"31","author":"Moll, R.; Franke, W.; Schiller, D.; Geiger, B.; Krepler, R.","year":"1982","unstructured":"Moll R, Franke W, Schiller D, Geiger B, Krepler R. The catalogue of human cytokeratins: patterns of expression in normal epithelia, tumours and cultured cells. Cell 1982;31:11-24.","journal-title":"Cell"},{"key":"ref_8","first-page":"90","article-title":"Papillary carcinoma. 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The differentiation of atypical adenomas and encapsulated follicular carcinomas in the thyroid gland. Virchows Arch (Pathol Anat) 1980;385: 125-41.","journal-title":"Virchows Arch (Pathol Anat)"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/j.1365-2559.1985.tb02435.x","article-title":"A study on the relationship between solid cell nests and mucoepidermoid carcinomas of the thyroid","volume":"9","author":"Harach, H.","year":"1985","unstructured":"Harach H. A study on the relationship between solid cell nests and mucoepidermoid carcinomas of the thyroid. Histopathology 1985;9:195-207.","journal-title":"Histopathology"},{"key":"ref_12","first-page":"35","article-title":"The use of monoclonal antibodies in histopathological diagnosis","volume":"12","author":"Gatter, K.C.; Falini, B.; Mason, D.Y.","year":"1984","unstructured":"Gatter KC, Falini B, Mason DY. The use of monoclonal antibodies in histopathological diagnosis. Rec Adv Histopathol 1984;12:35-67.","journal-title":"Rec Adv Histopathol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1177\/32.2.6198355","article-title":"Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP complexes)","volume":"32","author":"Cordell, J.L.; Falini, B.; Erber, W.N.","year":"1984","unstructured":"Cordell JL, Falini B, Erber WN, et al. Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP complexes). 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In: Cancer Cells: the transformed phenotype","author":"Osborn, M.; Altmannsberger, M.; Debus, E.; Weber, K.","year":"1984","unstructured":"Osborn M, Altmannsberger M, Debus E, Weber K. Conventional and monoclonal antibodies to intermediate filament proteins in human tumour diagnosis. In: Cancer Cells: the transformed phenotype. New York: Cold Spring Harbor Laboratory, 1984:191-200."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/BF02890169","article-title":"Monophasic synovial sarcoma of spindle cell type. Epithelial differentiation as revealed by ultrastructural features, content of prekeratin and binding of peanut agglutinin","volume":"44","author":"Miettinen, M.; Lehto, V.-P.; Virtanen, I.","year":"1983","unstructured":"Miettinen M, Lehto V-P, Virtanen I. Monophasic synovial sarcoma of spindle cell type. Epithelial differentiation as revealed by ultrastructural features, content of prekeratin and binding of peanut agglutinin. Virchows Arch (Cell Pathol) 1983;44: 187-99.","journal-title":"Virchows Arch (Cell Pathol)"},{"key":"ref_25","first-page":"435","article-title":"Keratin in epithelioid sarcoma. An immunohistochemical study","volume":"8","author":"Chase, D.R.; Enzinger, F.M.; Weiss, S.W.; Langloss, J.M.","year":"1985","unstructured":"Chase DR, Enzinger FM, Weiss SW, Langloss JM. Keratin in epithelioid sarcoma. An immunohistochemical study. Am J Surg Pathol 1985;8:435-41.","journal-title":"Am J Surg Pathol"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"950","DOI":"10.1136\/jcp.39.9.950","article-title":"Human lung tumours may coexpress different classes of intermediate filaments","volume":"39","author":"Gatter, K.C.; Dunnill, M.S.; van Muijen, G.N.P.; Mason, D.Y.","year":"1986","unstructured":"Gatter KC, Dunnill MS, van Muijen GNP, Mason DY. Human lung tumours may coexpress different classes of intermediate filaments. J Clin Pathol 1986;39:950-4.","journal-title":"J Clin Pathol"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1136\/jcp.39.4.435","article-title":"Epithelial membrane antigen and cytokeratin expression by meningiomas: an immunohistological study","volume":"39","author":"Theaker, J.M.; Gatter, K.C.; Esiri, M.M.; Fleming, K.A.","year":"1986","unstructured":"Theaker JM, Gatter KC, Esiri MM, Fleming KA. Epithelial membrane antigen and cytokeratin expression by meningiomas: an immunohistological study. J Clin Pathol 1986;39: 435-9.","journal-title":"J Clin Pathol"},{"key":"ref_28","first-page":"2618","article-title":"Coexpression of keratin and vimentin type intermediate filaments","volume-title":"human metastatic carcinoma cells. Proc Nail Acad Sci","volume":"80","author":"Raemakers, F.C.S.; Haag, D.; Kont, A.; Maesker, D.; Jap, P.H.K.; Vooijs, G.P.","year":"1983","unstructured":"Raemakers FCS, Haag D, Kont A, Maesker D, Jap PHK, Vooijs GP. Coexpression of keratin and vimentin type intermediate filaments in human metastatic carcinoma cells. Proc Nail Acad Sci USA 1983;80 2618-22."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/303701a0","article-title":"Coexpressin of vimentin and cytokeratins in parietal endoderm cells of early mouse embryo","volume":"303","author":"Lowe, E.B.; Hogan, B.L.M.; Kurkinen, M.; Garrels, J.I.","year":"1983","unstructured":"Lowe EB, Hogan BLM, Kurkinen M, Garrels JI. Coexpressin of vimentin and cytokeratins in parietal endoderm cells of early mouse embryo. Nature 1983;303:701-4.","journal-title":"Nature"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1007\/BF00509196","article-title":"Various keratin antibodies produce immunohistochemical staining of human myocardium and myometrium","volume":"83","author":"Huitfeld, H.S.; Brandzaeg, P.","year":"1985","unstructured":"Huitfeld HS, Brandzaeg P. Various keratin antibodies produce immunohistochemical staining of human myocardium and myometrium. Histochemistry 1985;83:381-9.","journal-title":"Histochemistry"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1002\/1097-0142(195209)5:5<966::AID-CNCR2820050512>3.0.CO;2-Z","article-title":"Squamous metaplasia of the thyroid gland","volume":"5","author":"Bullock, W.; Hummer, G.; Kahler, J.","year":"1952","unstructured":"Bullock W, Hummer G, Kahler J. Squamous metaplasia of the thyroid gland. Cancer 1952;5:966-74.","journal-title":"Cancer"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1136\/jcp.19.4.384","article-title":"Squamous epithelium in the human thyroid gland","volume":"19","author":"Harcourt-Webster, J.N.","year":"1966","unstructured":"Harcourt-Webster JN. Squamous epithelium in the human thyroid gland. J Clin Pathol 1966;19:384-8.","journal-title":"J Clin Pathol"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1097\/00000478-197806000-00002","article-title":"Squamous cells in the human thyroid gland","volume":"2","author":"LiVolsi, V.; Merino, M.","year":"1978","unstructured":"LiVolsi V, Merino M. Squamous cells in the human thyroid gland. Am J Surg Pathol 1978;2:133-40.","journal-title":"Am J Surg Pathol"},{"key":"ref_34","first-page":"984","article-title":"The coexpression of distinct classes of intermediate filaments in human neoplasms","volume":"109","author":"Gould, V.","year":"1985","unstructured":"Gould V. The coexpression of distinct classes of intermediate filaments in human neoplasms. Arch Pathol Lab Med 1985;109:984-5.","journal-title":"Arch Pathol Lab Med"},{"key":"ref_35","first-page":"2","article-title":"Farewell to the dual histogenesis of thyroid tumours","volume":"8","author":"Sobrinho-Simoes, M.; Nesland, J.M.; Johannesson, J.V.","year":"1985","unstructured":"Sobrinho-Simoes M, Nesland JM, Johannesson JV. Farewell to the dual histogenesis of thyroid tumours. Ultrastruct Pathol 1985;8:2-3.","journal-title":"Ultrastruct Pathol"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.40.2.136","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T15:43:18Z","timestamp":1765726998000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.40.2.136"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,2]]},"references-count":35,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1987,2,1]]},"published-print":{"date-parts":[[1987,2]]}},"alternative-id":["10.1136\/jcp.40.2.136"],"URL":"https:\/\/doi.org\/10.1136\/jcp.40.2.136","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,2]]}}}