{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:21:47Z","timestamp":1758846107904,"version":"3.44.0"},"reference-count":15,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1987,5]]},"abstract":"<jats:p>Passive haemagglutination inhibition (PHI) was adapted to quantitate red cell associated IgG. Twenty one patients with autoimmune haemolytic anaemia (AIHA) had a raised red cell associated IgG, mean (SD) = 5.783 (6.183) ng\/10(6) red blood cell compared with that of 69 subjects with a red cell associated IgG of 0.433 (0.349) ng\/10(6) red blood cell. Thirteen of 14 blood donors with a positive direct antiglobulin test (DAGT) had a normal red cell associated IgG. The only blood donor with positive DAGT and raised red cell associated IgG had AIHA. Studies of red cell associated IgG in other groups of patients were also undertaken. The technique is simple, does not require the use of sophisticated equipment, and is suitable as a routine test in hospital laboratories. The results of red cell associated IgG by PHI are reproducible and clinically relevant.<\/jats:p>","DOI":"10.1136\/jcp.40.5.494","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"494-499","source":"Crossref","is-referenced-by-count":2,"title":["Passive haemagglutination inhibition for quantitation of red cell associated IgG."],"prefix":"10.1136","volume":"40","author":[{"given":"H I","family":"Atrah","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J G","family":"Templeton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R J","family":"Crawford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G S","family":"Gabra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Mitchell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1987,5,1]]},"reference":[{"key":"ref_1","first-page":"133","article-title":"Incidence of a positive direct Coombs test in patients on alpha-methyldopa","volume":"ii:","author":"KC, I.Carstairs; A, Breckenridge; CT, Dollery; S, Worlledge","year":"1966","unstructured":"I Carstairs KC, Breckenridge A, Dollery CT, Worlledge S. Incidence of a positive direct Coombs test in patients on alpha-methyldopa. Lancet 1966;ii:133-5.","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/j.1423-0410.1980.tb02337.x","article-title":"Positive direct antiglobulin test in normal individuals","volume":"38","author":"Gorst, D.W.; Rawlinson, VI, Merry; AH, Stratton; F.","year":"1980","unstructured":"Gorst DW, Rawlinson VI, Merry AH, Stratton F. Positive direct antiglobulin test in normal individuals. Vox Sang 1980;38:99-105.","journal-title":"Vox Sang"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1111\/j.1365-2141.1978.tb01079.x","article-title":"The role of adherence to human mononuclear phagocytes in the destruction of red cells sensitised with non-complement binding IgG antibodies","volume":"38","author":"van der, Meulen F.W.; van der, Hart Mia; A, Fleer; von dem, Borne A.E.G.Kr; CP, Engelfriet; van, Loghem J.J.","year":"1978","unstructured":"Meulen FW van der, Hart Mia van der, Fleer A, Borne AEG Kr von dem, Engelfriet CP, Loghem JJ van. The role of adherence to human mononuclear phagocytes in the destruction of red cells sensitised with non-complement binding IgG antibodies. Br J Haematol 1978;38:541-9.","journal-title":"Br J Haematol"},{"key":"ref_4","first-page":"17","article-title":"Positive direct antiglobulin test in normal individuals II","volume":"5","author":"Stratton, F.; Rawlinson, VI, Merry; AH, Thomson; E.E.","year":"1983","unstructured":"Stratton F, Rawlinson VI, Merry AH, Thomson EE. Positive direct antiglobulin test in normal individuals II. Clin Lab Haematol 1983;5:17-21.","journal-title":"Clin Lab Haematol"},{"key":"ref_5","first-page":"393","article-title":"A quantitative antiglobulin test for IgG for use in blood transfusion serology","volume":"4","author":"Merry, A.H.; Thomson, E.E.; Rawlinson, VI, Stratton; F.","year":"1982","unstructured":"Merry AH, Thomson EE, Rawlinson VI, Stratton F. A quantitative antiglobulin test for IgG for use in blood transfusion serology. Clin Lab Haematol 1982;4:393-402.","journal-title":"Clin Lab Haematol"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1056\/NEJM197107292850503","article-title":"Red cell antibodies in acquired haemolytic anaemia with negative antiglobulin serum test","volume":"285","author":"Gilliland, B.C.; Baxter, E.; Evans, R.C.","year":"1971","unstructured":"Gilliland BC, Baxter E, Evans RC. Red cell antibodies in acquired haemolytic anaemia with negative antiglobulin serum test. N Engl J Med 1971;285:252-6.","journal-title":"N Engl J Med"},{"key":"ref_7","first-page":"155","article-title":"Quantitation of RBC-bound immunoglobulin and complement components","volume-title":"haemolytic anaemias","author":"Schreibner, A.D.","year":"1985","unstructured":"Schreibner AD. Quantitation of RBC-bound immunoglobulin and complement components. In: Chaplin Jr H, ed. Immune haemolytic anaemias. Edinburgh: Churchill Livingston, 1985:155-75."},{"key":"ref_8","first-page":"29","article-title":"Treatment of autoimmune thrombocytopenic purpura with rhesus antibodies","volume":"49","author":"Salama, A.; Kiefel, V.; Amberg, R.; Mueller-Eckhardt, C.","year":"1984","unstructured":"Salama A, Kiefel V, Amberg R, Mueller-Eckhardt C. Treatment of autoimmune thrombocytopenic purpura with rhesus antibodies (anti-Rho [D]). Blut 1984;49:29-35."},{"issue":"2","key":"ref_9","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/0092-8674(74)90120-2","article-title":"A haemagglutination inhibition technique for detection of immunoglobulin in supernatants of human lymphoblastoid cell lines","volume":"3","author":"Evans, J.; Steel, M.; Arthur, E.","year":"1974","unstructured":"Evans J, Steel M, Arthur E. A haemagglutination inhibition technique for detection of immunoglobulin in supernatants of human lymphoblastoid cell lines. Cell 1974;3(2): 153-8.","journal-title":"Cell"},{"key":"ref_10","first-page":"339","article-title":"Modulation of suppressor T-cells for the treatment of aplastic anaemia","volume":"ii:","author":"Atrah, H.I.; Crawford, R.J.; Gabra, G.S.","year":"1985","unstructured":"Atrah HI, Crawford RJ, Gabra GS, et al. Modulation of suppressor T-cells for the treatment of aplastic anaemia. Lancet 1985;ii:339-440.","journal-title":"Lancet"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1046\/j.1537-2995.1984.24685066803.x","article-title":"Quantitation of red cell-associated IgG using an immunoradiometric assay","volume":"24","author":"Jeje, M.O.; Blajchman, M.A.; Steeves, K.; Horsewood, P.; Kelton, J.G.","year":"1984","unstructured":"Jeje MO, Blajchman MA, Steeves K, Horsewood P, Kelton JG. Quantitation of red cell-associated IgG using an immunoradiometric assay. Transfusion 1984;24:473-6.","journal-title":"Transfusion"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1182\/blood.V55.1.48.48","article-title":"Red blood cell associated IgG in normal and pathological states","volume":"55","year":"1980","unstructured":"Szymanski 10, Odgren PR, Normand LF, Snyder LM. Red blood cell associated IgG in normal and pathological states. Blood 1980;55:48-54.","journal-title":"Blood"},{"key":"ref_13","first-page":"675","article-title":"The use of purified 1251-labelled anti-yglobulin in the determination of the number of D antigen sites on red cells of different phenotypes","volume":"10","author":"Rochna, E.; Hughes-Jones, N.C.","year":"1965","unstructured":"Rochna E, Hughes-Jones NC. The use of purified 1251-labelled anti-yglobulin in the determination of the number of D antigen sites on red cells of different phenotypes. Vox Sang 1965;10:675-86.","journal-title":"Vox Sang"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"437","DOI":"10.7326\/0003-4819-85-4-437","article-title":"Felty's Syndrome: granulocyte-bound immunoglobulin G and splenectomy","volume":"85","author":"Lougue, G.","year":"1976","unstructured":"Lougue G. Felty's Syndrome: granulocyte-bound immunoglobulin G and splenectomy. Ann Intern Med 1976;85:437-42.","journal-title":"Ann Intern Med"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1182\/blood.V55.3.424.424","article-title":"Comparison of two direct assays for platelet associated IgG (PAIgG) in assessment of immune and non-immune thrombocytopenia","volume":"55","author":"Kelton, J.G.; Giles, A.R.; Neame, P.B.; Powers, P.; Hageman, N.; Hirsh, J.","year":"1980","unstructured":"Kelton JG, Giles AR, Neame PB, Powers P, Hageman N, Hirsh J. Comparison of two direct assays for platelet associated IgG (PAIgG) in assessment of immune and non-immune thrombocytopenia. Blood 1980;55:424-9.","journal-title":"Blood"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.40.5.494","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:28:36Z","timestamp":1758821316000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.40.5.494"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,5]]},"references-count":15,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1987,5,1]]},"published-print":{"date-parts":[[1987,5]]}},"alternative-id":["10.1136\/jcp.40.5.494"],"URL":"https:\/\/doi.org\/10.1136\/jcp.40.5.494","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1987,5]]}}}