{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:45:18Z","timestamp":1773420318409,"version":"3.50.1"},"reference-count":33,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1987,5]]},"abstract":"<jats:p>The characteristics of cholesterol uptake by 83 human gall bladders (obtained at cholecystectomy) were studied with a modified Ussing technique. Real and artificial biles labelled with 14C-cholesterol and 3H-dextran (the latter to correct for adherent mucosal bile) were used; all gall bladders absorbed cholesterol (average 3.5 nmol\/cm2\/minute). Recovery of the absorbed cholesterol from the tissue showed that about 4% was esterified over 60 minutes. In artificial bile the rate of absorption of cholesterol increased as the bile saturation index rose, but became constant once supersaturation was achieved. In contrast, supersaturated real bile permitted greater absorption of cholesterol, possibly due to enhanced cholesterol solubilisation. Preincubation of gall bladder tissue in sodium cyanide (5 mM) caused a 30% reduction in cholesterol uptake indicating that, although absorption is predominantly a \"passive\" process, there is a partial \"active\" component. There were no pronounced differences in the rate of cholesterol absorption as gall bladders became more diseased, but there was a reduction in the amount of cholesterol ester formed.<\/jats:p>","DOI":"10.1136\/jcp.40.5.524","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"524-529","source":"Crossref","is-referenced-by-count":40,"title":["Characteristics of cholesterol absorption by human gall bladder: relevance to cholesterolosis."],"prefix":"10.1136","volume":"40","author":[{"given":"M R","family":"Jacyna","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P E","family":"Ross","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M A","family":"Bakar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Hopwood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I A","family":"Bouchier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1987,5,1]]},"reference":[{"issue":"324","key":"ref_1","first-page":"1","article-title":"Cholesterosis of the gall-bladder: a clinical study based on 269 cholecystectomies","volume":"1964","author":"Salmenkivi, K.","unstructured":"Salmenkivi K. Cholesterosis of the gall-bladder: a clinical study based on 269 cholecystectomies. Acta Chir Scand 1964;324 (suppl): 1-93.","journal-title":"Acta Chir Scand"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/S0016-5085(19)36106-2","article-title":"Cholesterosis of the gallbladder: an autopsy study of 165 cases","volume":"27","author":"Feldman, M.; M, Jr, Feldman","year":"1954","unstructured":"Feldman M, Feldman M Jr. Cholesterosis of the gallbladder: an autopsy study of 165 cases. Gastroenterology 1954;27:641-8.","journal-title":"Gastroenterology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/BF00490366","article-title":"Electron microscopic observations on the cholesterol distributed in the epithelial cells of the gallbladder","volume":"44","author":"Koga, A.; Todo, S.; Nishimura, M.","year":"1975","unstructured":"Koga A, Todo S, Nishimura M. 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Pathogenetic interrelations between cholesterolosis and cholesterol gallstone disease. Review Roumanian Medecin 1983;21:175-9.","journal-title":"Review Roumanian Medecin"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/S0022-2275(20)36995-9","article-title":"Absorption of cholesterol by the gall bladder","volume":"17","author":"Neiderhiser, D.H.; Harmon, C.K.; Roth, P.H.","year":"1976","unstructured":"Neiderhiser DH, Harmon CK, Roth PH. Absorption of cholesterol by the gall bladder. J Lipid Res 1976;17:117-24.","journal-title":"J Lipid Res"},{"key":"ref_9","series-title":"health and disease: proceedings of the sixth British Society of Gastroenterology\/ Smith Kline & French international workshop 1985. 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Amsterdam: Elsevier, 1985:159."}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.40.5.524","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:19:10Z","timestamp":1758817150000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.40.5.524"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,5]]},"references-count":33,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1987,5,1]]},"published-print":{"date-parts":[[1987,5]]}},"alternative-id":["10.1136\/jcp.40.5.524"],"URL":"https:\/\/doi.org\/10.1136\/jcp.40.5.524","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,5]]}}}