{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T03:58:01Z","timestamp":1765598281216,"version":"3.48.0"},"reference-count":19,"publisher":"BMJ","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1987,8]]},"abstract":"<jats:p>Aerobic and anaerobic culture of sputum on selective bacteriological media, combined with a new method of plating and plate reading, permitted rapid identification and quantitation of three genera of bacteria commonly associated with chronic bronchial sepsis (Haemophilus spp, Pseudomonas aeruginosa, and Staphylococcus aureus) and avoided time consuming serial dilution of sputum and subculture of organisms. The accuracy of this new technique was assessed in patients with chronic bronchial sepsis and was used to detect changes in the colonising microbial load of Haemophilus spp and Ps aeruginosa in patients with bronchiectasis receiving one of three different antibiotic regimens: intermittent seven day courses of amoxycillin for exacerbations; or a six month course of continuous oral or nebulised amoxycillin. The colonising microbial load of Haemophilus spp was reduced only temporarily (+++ to ++) after each intermittent course of antibiotic, but a sustained and greater reduction in the colonising microbial load of both Haemophilus spp (+++ to +) and antibiotic resistant P aeruginosa (+++ to +) was seen during both continuous treatments. Sputum purulence decreased in parallel with colonising microbial load, reflecting a reduction in host inflammatory response to the colonising microbial load.<\/jats:p>","DOI":"10.1136\/jcp.40.8.830","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"830-836","source":"Crossref","is-referenced-by-count":12,"title":["Simple method of monitoring colonising microbial load in chronic bronchial sepsis: pilot comparison of reduction in colonising microbial load with antibiotics given intermittently and continuously."],"prefix":"10.1136","volume":"40","author":[{"given":"D C","family":"Currie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Higgs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Metcalfe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D E","family":"Roberts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P J","family":"Cole","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1987,8,1]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"A new look at the pathogenesis and management of persistent bronchial sepsis: a \"vicious circle\" hypothesis and its logical therapeutic connotations. In: Strategiesfor the management of chronic bronchial sepsis","author":"PJ, I.Cole","year":"1984","unstructured":"I Cole PJ. A new look at the pathogenesis and management of persistent bronchial sepsis: a \"vicious circle\" hypothesis and its logical therapeutic connotations. In: Strategiesfor the management of chronic bronchial sepsis. Oxford: Medicine Publishing Foundation, 1984:1-20."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1016\/S0140-6736(80)91295-7","article-title":"Use of selective media in bacteriological investigation of patients with chronic suppurative respiratory infection","volume":"i:","author":"Roberts, D.E.; Cole, P.","year":"1980","unstructured":"Roberts DE, Cole P. Use of selective media in bacteriological investigation of patients with chronic suppurative respiratory infection. 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Lancet 1953;ii:534-7.","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/S0041-3879(64)80051-9","article-title":"N-acetyl-L-cysteine as liquefying agent in the bacteriological examination of sputum","volume":"45","author":"Mead, G.R.; Woodhams, A.W.","year":"1964","unstructured":"Mead GR, Woodhams AW. N-acetyl-L-cysteine as liquefying agent in the bacteriological examination of sputum. Tubercle 1964;45:370-3.","journal-title":"Tubercle"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/S0140-6736(53)90275-X","article-title":"Liquefaction of sputum for bacteriological examination","volume":"ii:","author":"Rawlins, G.A.","year":"1953","unstructured":"Rawlins GA. Liquefaction of sputum for bacteriological examination. Lancet 1953;ii:538-9.","journal-title":"Lancet"},{"key":"ref_7","first-page":"1306","article-title":"An evaluation of sputum examination in chronic bronchitis","volume":"ii:","author":"Brumfitt, W.; Willoughby, M.L.N.; Bromley, L.L.","year":"1957","unstructured":"Brumfitt W, Willoughby MLN, Bromley LL. An evaluation of sputum examination in chronic bronchitis. Lancet 1957;ii: 1306-9.","journal-title":"Lancet"},{"key":"ref_8","first-page":"112","article-title":"Bacteriology of lower respiratory tract secretions in \"normals\" and chronic bronchitics","volume":"ii:","author":"Lees, A.W.; McNaught, W.","year":"1959","unstructured":"Lees AW, McNaught W. Bacteriology of lower respiratory tract secretions in \"normals\" and chronic bronchitics. Lancet 1959; ii: 112-5.","journal-title":"Lancet"},{"key":"ref_9","first-page":"1019","article-title":"Bacteriology of expectorated sputum with quantitative culture and wash technique compared to transtracheal aspirates","volume":"117","author":"Bartlett, J.G.; Finegold, S.M.","year":"1978","unstructured":"Bartlett JG, Finegold SM. Bacteriology of expectorated sputum with quantitative culture and wash technique compared to transtracheal aspirates. Am Rev Respir Dis 1978;117:1019-27.","journal-title":"Am Rev Respir Dis"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1016\/S0140-6736(65)90572-6","article-title":"Value of dilute inocula in cultural examination of sputum","volume":"ii:","author":"Dixon, J.M.S.; Miller, D.C.","year":"1965","unstructured":"Dixon JMS, Miller DC. Value of dilute inocula in cultural examination of sputum. Lancet 1965;ii: 1046-8.","journal-title":"Lancet"},{"issue":"18","key":"ref_11","first-page":"214","article-title":"Quantitation ofmicro-organisms in sputum","volume":"1969","author":"Monroe, P.W.; Muchmore, H.G.; Felton, F.G.; Pirtle, J.K.","unstructured":"Monroe PW, Muchmore HG, Felton FG, Pirtle JK. Quantitation ofmicro-organisms in sputum. Appi Microbiol 1969;18:214-20.","journal-title":"Appi Microbiol"},{"issue":"100","key":"ref_12","first-page":"831","article-title":"Diagnostic and therapeutic advantages of serial quantitative cultures of fresh sputum in acute bacterial pneumonia","volume":"1969","author":"Pirtle, J.K.; Monroe, P.W.; Mohr, Smalley T.K.; JA, Rhoades; E.R.","unstructured":"Pirtle JK, Monroe PW, Smalley TK. Mohr JA, Rhoades ER. Diagnostic and therapeutic advantages of serial quantitative cultures of fresh sputum in acute bacterial pneumonia. Am Rev Respir Dis 1969;100:831-8.","journal-title":"Am Rev Respir Dis"},{"key":"ref_13","first-page":"760","article-title":"An evaluation of some methods for culturing sputum from patients with bronchitis and emphysema","volume":"89","author":"Lapinski, E.M.; Flakas, E.D.; Taylor, B.C.","year":"1964","unstructured":"Lapinski EM, Flakas ED, Taylor BC. An evaluation of some methods for culturing sputum from patients with bronchitis and emphysema. Am Rev Respir Dis 1964;89:760-3.","journal-title":"Am Rev Respir Dis"},{"issue":"98","key":"ref_14","first-page":"810","article-title":"Quantitative bacteriology of sputum","volume":"1968","unstructured":"Kilboum JP, Campbell RA, Grach JL, Willis MD. Quantitative bacteriology of sputum. 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N Z Med J 1973;78:442-3.","journal-title":"N Z Med J"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1136\/bmj.289.6456.1409","article-title":"Isolation of spheroplastic forms of Haemophilus influenzae from sputum in conventionally treated chronic bronchial sepsis using selective medium supplemented with N-acetyl-D-glucosamine: a possible reservoir for re-emergence of infection","volume":"289","author":"Roberts, D.; Higgs, E.; Rutman, A.; Cole, P.","year":"1984","unstructured":"Roberts D, Higgs E, Rutman A, Cole P. Isolation of spheroplastic forms of Haemophilus influenzae from sputum in conventionally treated chronic bronchial sepsis using selective medium supplemented with N-acetyl-D-glucosamine: a possible reservoir for re-emergence of infection. 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Reduction of ciliary beat frequency in vitro by sputum from patients with bronchiectasis: a serine proteinase effect. Thorax 1984;39:663-7.","journal-title":"Thorax"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.40.8.830","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T03:54:32Z","timestamp":1765598072000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.40.8.830"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,8]]},"references-count":19,"journal-issue":{"issue":"8","published-online":{"date-parts":[[1987,8,1]]},"published-print":{"date-parts":[[1987,8]]}},"alternative-id":["10.1136\/jcp.40.8.830"],"URL":"https:\/\/doi.org\/10.1136\/jcp.40.8.830","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1987,8]]}}}