{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T04:24:01Z","timestamp":1765599841076,"version":"3.48.0"},"reference-count":8,"publisher":"BMJ","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1988,12]]},"abstract":"<jats:p>The use of gas chromatography and mass spectrometry with selected ion monitoring detected tuberculostearic acid (TBSA) in 10 of 12 formalin fixed, paraffin wax embedded nasopharyngeal and head and neck biopsy specimens from patients with confirmed tuberculosis and carcinoma, and in one of 50 control specimens (giving a sensitivity of 83% and a specificity of 98%). The two false negative cases had very small tissue fragments and the patient with a false positive result may have had pulmonary tuberculosis. Tuberculostearic acid (TBSA) was also detected in nine of 16 specimens from the head and neck region with non-caseating granulomas suspected, but not confirmed, to be tuberculosis. It is concluded that nasopharyngeal tuberculosis is relatively common in Hong Kong and should be considered when biopsy specimens show granulomas. The detection of TBSA in tissue biopsy specimens is a useful, rapid method for the diagnosis of tuberculosis and other mycobacterial infections, and can be conveniently performed within two days on formalin fixed and paraffin wax embedded material.<\/jats:p>","DOI":"10.1136\/jcp.41.12.1334","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"1334-1336","source":"Crossref","is-referenced-by-count":4,"title":["Diagnosis of nasopharyngeal tuberculosis by detection of tuberculostearic acid in formalin fixed, paraffin wax embedded tissue biopsy specimens."],"prefix":"10.1136","volume":"41","author":[{"given":"M","family":"Arnold","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C Y","family":"Chan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S W","family":"Cheung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C A","family":"Van Hasselt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G L","family":"French","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1988,12,1]]},"reference":[{"article-title":"Director of Medical and Health Services, 1986-1987. Departmental report. Hong Kong Government","year":"1987","key":"ref_1","unstructured":"Director of Medical and Health Services, 1986-1987. Departmental report. Hong Kong Government, Hong Kong, 1987."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1136\/jcp.36.7.723","article-title":"Granulomatous inflammation-a review","volume":"36","author":"Williams, G.T.; Williams, W.J.","year":"1984","unstructured":"Williams GT, Williams WJ. Granulomatous inflammation-a review. J Clin Pathol 1984;36:723-33.","journal-title":"J Clin Pathol"},{"key":"ref_3","first-page":"1079","article-title":"Granulomas of liver","volume":"1","author":"Anonymous","year":"1975","unstructured":"Anonymous. Granulomas of liver. [Editorial] Lancet 1975; i1:1079-80.","journal-title":"[Editorial] Lancet"},{"key":"ref_4","article-title":"Gas chromatography\/mass spectrometry applications in microbiology","author":"Larsson, L.; Mardh, P.A.; Odham, G.","year":"1984","unstructured":"Larsson L, Mardh PA, Odham G. Gas chromatography\/mass spectrometry applications in microbiology. Plenum: New York, 1984."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0378-4347(00)81468-1","article-title":"Detection of tuberculostearic acid in mycobacteria and nocardiae by gas chromatography and mass spectrometry using selected ion monitoring","volume":"13","author":"Larsson, L.; Mardh, P.A.; Odham, G.","year":"1979","unstructured":"Larsson L, Mardh PA, Odham G. Detection of tuberculostearic acid in mycobacteria and nocardiae by gas chromatography and mass spectrometry using selected ion monitoring. 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Diagnosis of pulmonary tuberculosis by detection of tuberculostearic acid in sputum by using gas chromatography-mass spectrometry with selected ion monitoring. J Infect Dis 1987;156:356-62.","journal-title":"J Infect Dis"},{"key":"ref_8","first-page":"17","article-title":"Diagnosis of tuberculous meningitis by detection of tuberculostearic acid in cerebrospinal fluid","volume":"1","author":"French, G.L.; Teoh, R.; Chan, C.Y.; Humphries, M.J.; Cheung, S.W.; O'Mahoney, G.","year":"1987","unstructured":"French GL, Teoh R, Chan CY, Humphries MJ, Cheung SW, O'Mahoney G. Diagnosis of tuberculous meningitis by detection of tuberculostearic acid in cerebrospinal fluid. Lancet 1987;ii:1 17-9.","journal-title":"Lancet"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.41.12.1334","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T04:21:16Z","timestamp":1765599676000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.41.12.1334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,12]]},"references-count":8,"journal-issue":{"issue":"12","published-online":{"date-parts":[[1988,12,1]]},"published-print":{"date-parts":[[1988,12]]}},"alternative-id":["10.1136\/jcp.41.12.1334"],"URL":"https:\/\/doi.org\/10.1136\/jcp.41.12.1334","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1988,12]]}}}