{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T00:13:27Z","timestamp":1758932007077,"version":"3.44.0"},"reference-count":17,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1988,5]]},"abstract":"<jats:p>A simple and rapid method for the measurement of cobalamin bound intrinsic factor (Cbl-IF) complex and intrinsic factor binding antibody is described. The method is based on the principle of affinity chromatography and adapted to a batch separation technique. A specific ligand staphylococcal protein A was coupled to Sepharose to form a convenient solid phase matrix. The intrinsic factor binding antibody in patients with pernicious anaemia was used to form an immune complex with Cbl-IF. This complex was adsorbed on to staphylococcal protein A. Gastric juice from control subjects and patients with pernicious anaemia was assayed for intrinsic factor activity and the results correlated very closely with two other established methods. Sera from 30 control subjects were assayed for binding intrinsic factor antibody and all were found to be negative; of 15 patients with pernicious anaemia, six were positive. These patients were selected with blocking antibody. The method does not require technologically advanced protein separation techniques and could therefore be applied in any clinical laboratory using radioisotopes. It could also be adapted to assay cobalamin in body fluids or in food.<\/jats:p>","DOI":"10.1136\/jcp.41.5.568","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"568-572","source":"Crossref","is-referenced-by-count":6,"title":["Rapid protein A assay for intrinsic factor and its binding antibody."],"prefix":"10.1136","volume":"41","author":[{"given":"N A","family":"Sourial","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1988,5,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1016\/S0140-6736(63)90736-0","article-title":"A method for the assay of human gastric intrinsic factor and for the detection and titration of antibodies against intrinsic factor","volume":"ii:","author":"Ardeman, S.; Chanarin, I.","year":"1963","unstructured":"Ardeman S, Chanarin I. A method for the assay of human gastric intrinsic factor and for the detection and titration of antibodies against intrinsic factor. Lancet 1963;ii: 1350-4.","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1182\/blood.V25.6.875.875","article-title":"Rapid charcoal assay for intrinsic factor (IF), gastric juice unsaturated B,2 binding capacity, antibody to IF, and serum unsaturated BR2 binding capacity","volume":"25","author":"Gottlieb, C.; Lau, K.S.; Wasserman, L.R.; Herbert, V.","year":"1965","unstructured":"Gottlieb C, Lau KS, Wasserman LR, Herbert V. Rapid charcoal assay for intrinsic factor (IF), gastric juice unsaturated B,2 binding capacity, antibody to IF, and serum unsaturated BR2 binding capacity. Blood 1965;25:875-83. Sourial","journal-title":"Blood"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1182\/blood.V53.4.788.788","article-title":"An assay for intrinsic factor based on blocking of the R binder of gastric juice by cobinamide","volume":"53","author":"Begley, J.A.; Trachtenberg, A.","year":"1979","unstructured":"Begley JA, Trachtenberg A. An assay for intrinsic factor based on blocking of the R binder of gastric juice by cobinamide. Blood 1979;53:788-93.","journal-title":"Blood"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1136\/jcp.25.4.320","article-title":"A simple assay of intrinsic factor-vitamin B12 complex employing the binding intrinsic factor antibody","volume":"25","author":"Jacob, E.; O'Brien, H.A.W.","year":"1972","unstructured":"Jacob E, O'Brien HAW. A simple assay of intrinsic factor-vitamin B12 complex employing the binding intrinsic factor antibody. 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J Lab Clin Med 1966;67:879-90.","journal-title":"J Lab Clin Med"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1093\/clinchem\/28.8.1794","article-title":"Functional activity of intrinsic factor measured by using solubilized receptor protein","volume":"28","author":"Marcoulis, G.; Rothenberg, S.P.","year":"1982","unstructured":"Marcoulis G, Rothenberg SP. Functional activity of intrinsic factor measured by using solubilized receptor protein. Clin Chem 1982;28:1794-6.","journal-title":"Clin Chem"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"140","DOI":"10.4049\/jimmunol.104.1.140","article-title":"Phylogenetic insight into evolution of mammalian fragment of y G globulin using staphylococcal protein A","volume":"104","author":"Kronvall, G.; Seal, U.S.; Finstad, S.; Jnr, Williams R.C.","year":"1970","unstructured":"Kronvall G, Seal US, Finstad S, Williams RC Jnr. Phylogenetic insight into evolution of mammalian fragment of y G globulin using staphylococcal protein A. J Immunol 1970;104:140-7.","journal-title":"J Immunol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/0022-1759(78)90131-X","article-title":"protein A: a tracer for general use in immunoassay","volume":"24","author":"Langone, J.J.","year":"1978","unstructured":"Langone JJ. (125I) protein A: a tracer for general use in immunoassay. J Immunol Methods 1978;24:269-85.","journal-title":"J Immunol Methods"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1172\/JCI109083","article-title":"Correction of cobalamin malabsorption in pancreatic insufficiency with a cobalamin analogue that binds with high affinity to R protein but not to intrinsic factor. 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