{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T13:48:43Z","timestamp":1758808123164,"version":"3.44.0"},"reference-count":11,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1988,6]]},"abstract":"<jats:p>The use of a faecal preservative and several staining methods, together with formalin ether concentration, were evaluated for the improved diagnosis of intestinal amoebiasis and giardiasis in 1285 patients with diarrhoea or dysentery and from asymptomatic controls. All samples were screened by three wet mount techniques. Thirty eight specimens of diarrhoeal or dysenteric stool were preserved in polyvinyl alcohol (PVA) and stained by trichrome and Spencer and Monroe short iron haematoxylin stain. Thirty nine preserved faecal samples submitted for routine screening were subjected to formalin ether concentration, wet mount examination, and permanent staining. Saline and buffered methylene blue (BMB) mounts were equally good for detection of trophozoite Entamoebae while Giardia trophozoites were detected only by the saline mount. The iodine mount was superior to the other mounts for protozoan cyst detection. The concentration procedure enhanced cyst recovery. Faecal preservation and subsequent staining was superior to wet mount examination for detection of the trophozoite stage and avoided the need for fresh specimens. Both the trichrome and the iron haematoxylin stains were comparable for the detection of cysts and trophozoites of the Entomoebae. Giardia lamblia trophozoites stained better with iron haematoxylin than with the trichrome. Preservation and permanent staining is recommended as the most productive means for the accurate identification of the various protozoan parasites.<\/jats:p>","DOI":"10.1136\/jcp.41.6.694","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"694-699","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of faecal preservation and staining methods in the diagnosis of acute amoebiasis and giardiasis."],"prefix":"10.1136","volume":"41","author":[{"given":"N","family":"Shetty","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Prabhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1988,6,1]]},"reference":[{"key":"ref_1","first-page":"417","volume":"63","author":"Davis, A.; Pawlowski, Z.S.","year":"1985","unstructured":"Davis A, Pawlowski ZS. Amoebiasis and its control. Bull WHO 1985;63:417-26.","journal-title":"Amoebiasis and its control. Bull WHO"},{"key":"ref_2","first-page":"478","article-title":"The laboratory diagnosis ofamoebiasis","volume":"47","author":"Healy, G.R.","year":"1971","unstructured":"Healy GR. The laboratory diagnosis ofamoebiasis. Bull N Y Acad Med 1971;47:478-93.","journal-title":"Bull N Y Acad Med"},{"key":"ref_3","first-page":"459","article-title":"Diagnostic clinical parasitology I. Proper specimen collection and processing","volume":"46","author":"Garcia, L.S.; Voge, M.","year":"1980","unstructured":"Garcia LS, Voge M. Diagnostic clinical parasitology I. Proper specimen collection and processing. Am J Med Technol 1980;46:459-67.","journal-title":"Am J Med Technol"},{"key":"ref_4","first-page":"174","article-title":"The use and limitations of the indirect haemagglutination test in the diagnosis ofintestinal amoebiasis","volume":"5","author":"Healy, G.R.","year":"1968","unstructured":"Healy GR. The use and limitations of the indirect haemagglutination test in the diagnosis ofintestinal amoebiasis. Health Lab Sci 1968;5: 174-9.","journal-title":"Health Lab Sci"},{"key":"ref_5","first-page":"84","article-title":"Diagnosis of giardiasis by specific IgM antibody ELISA","volume":"1","author":"Goka, A.K.J.; Rolston, D.D.K.; Mathan, VI, Farthing; M.J.G.","year":"1986","unstructured":"Goka AKJ, Rolston DDK, Mathan VI, Farthing MJG. Diagnosis of giardiasis by specific IgM antibody ELISA. Lancet 1986;ii: 1 84-6.","journal-title":"Lancet"},{"key":"ref_6","first-page":"691","article-title":"Immunodiagnostic detection of Giardia antigen in faeces by a rapid visual enzyme linked immunosorbent assay","volume":"ii:","author":"Green, E.L.; Miles, M.A.; Warhurst, D.C.","year":"1985","unstructured":"Green EL, Miles MA, Warhurst DC. Immunodiagnostic detection of Giardia antigen in faeces by a rapid visual enzyme linked immunosorbent assay. Lancet 1985;ii:691-3.","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/0035-9203(87)90436-6","article-title":"An enzyme-linked immunosorbent assay for the detection of Entamoeba histolytica antigens in faecal material","volume":"81","author":"Grundy, M.S.; Voller, A.; Warhurst, D.","year":"1987","unstructured":"Grundy MS, Voller A, Warhurst D. An enzyme-linked immunosorbent assay for the detection of Entamoeba histolytica antigens in faecal material. Trans R Soc Trop Med Hyg 1987;81:627-32.","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1038\/1721051a0","article-title":"Rapid staining of intestinal amoebae on wet mounts","volume":"172","author":"Nair, C.P.","year":"1953","unstructured":"Nair CP. Rapid staining of intestinal amoebae on wet mounts. Nature 1953;172:1051.","journal-title":"Nature"},{"key":"ref_9","first-page":"457","article-title":"Laboratory diagnosis of parasitic infections","author":"Garcia, L.S.","year":"1982","unstructured":"Garcia LS. Laboratory diagnosis of parasitic infections. In: Finegold SM, Martin WJ, eds. Diagnostic microbiology. St Louis: The CV Mosby Co, 1982:457-75."},{"key":"ref_10","first-page":"932","article-title":"A comparison of the formalin ether concentration and trichrome stained smear methods for the recovery and identification of intestinal protozoa","volume":"45","author":"Garcia, L.S.; Brewer, T.C.; Bruckner, D.A.","year":"1979","unstructured":"Garcia LS, Brewer TC, Bruckner DA. A comparison of the formalin ether concentration and trichrome stained smear methods for the recovery and identification of intestinal protozoa. Am J Med Technol 1979;45:932-5.","journal-title":"Am J Med Technol"},{"key":"ref_11","first-page":"1554","article-title":"Polyvinyl alcohol fixative as a preservative and adhesive for protozoa in dysenteric stools and other liquid material","volume":"34","author":"Brooke, M.M.; Goldman, M.","year":"1949","unstructured":"Brooke MM, Goldman M. Polyvinyl alcohol fixative as a preservative and adhesive for protozoa in dysenteric stools and other liquid material. J Lab Clin Med 1949;34:1554-60.","journal-title":"J Lab Clin Med"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.41.6.694","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T13:03:50Z","timestamp":1758805430000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.41.6.694"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,6]]},"references-count":11,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1988,6,1]]},"published-print":{"date-parts":[[1988,6]]}},"alternative-id":["10.1136\/jcp.41.6.694"],"URL":"https:\/\/doi.org\/10.1136\/jcp.41.6.694","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[1988,6]]}}}