{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:16:50Z","timestamp":1791044210102,"version":"4.1.0"},"reference-count":37,"publisher":"BMJ","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[1988,7]]},"abstract":"<jats:p>A new monoclonal antibody, Y1\/82A, was raised against phytohaemagglutinin activated peripheral blood mononuclear cells. Using an immunohistochemical technique it was shown that Y1\/82A reacts against peripheral blood and bone marrow monocytes and resident macrophages from essentially all human tissues. Y1\/82A bound to determinants present in leukaemic cells from patients with acute myelomonocytic leukaemia and acute monocytic leukaemia, but not to neoplastic cells from patients with malignant lymphoproliferative disorders or malignant epithelial tumours. Y1\/82A failed to react with other cell types, with the exception of osteoclasts and megakaryocytes. Analysis by Western blotting showed that the antigen detected by antibody Y1\/82A was associated with intracellular granules in macrophages. Monoclonal antibody Y1\/82A may be useful in the diagnosis of monocytic leukaemias and histiocytic neoplasms and in the identification of macrophages in tissues from various inflammatory and neoplastic conditions.<\/jats:p>","DOI":"10.1136\/jcp.41.7.753","type":"journal-article","created":{"date-parts":[[2007,6,9]],"date-time":"2007-06-09T00:50:46Z","timestamp":1181350246000},"page":"753-758","source":"Crossref","is-referenced-by-count":42,"title":["Monoclonal antibody (Y1\/82A) with specificity towards peripheral blood monocytes and tissue macrophages."],"prefix":"10.1136","volume":"41","author":[{"given":"F R","family":"Davey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J L","family":"Cordell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W N","family":"Erber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K A","family":"Pulford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K C","family":"Gatter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D Y","family":"Mason","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1988,7,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"78","DOI":"10.7326\/0003-4819-88-1-78","article-title":"Monocytes and macrophages: Functions and diseases","volume":"88","author":"MJ, I.Cline; RI, Lehrer; MC, Territo; DW, Golde","year":"1978","unstructured":"I Cline MJ, Lehrer RI, Territo MC, Golde DW. Monocytes and macrophages: Functions and diseases. Ann Intern Med 1978;88:78-88.","journal-title":"Ann Intern Med"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/j.1600-065X.1980.tb01042.x","article-title":"Dendritic cells: features and functions","volume":"53","author":"Steinman, R.M.; Nussenzweig, M.C.","year":"1980","unstructured":"Steinman RM, Nussenzweig MC. Dendritic cells: features and functions. Immunol Rev 1980;53:127-47.","journal-title":"Immunol Rev"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1056\/NEJM197910043011404","article-title":"Hematopoietic stem cells","volume":"301","author":"Quesenberry, P.; Levitt, L.","year":"1979","unstructured":"Quesenberry P, Levitt L. Hematopoietic stem cells. N Engi J Med 1979;301:755-60,819-23,868-72.","journal-title":"N Engi J Med"},{"key":"ref_4","first-page":"937","article-title":"Bone marrow origin of hepatic macrophages (Kupffer cells) in humans","author":"Gale, R.P.; Sparkes, R.S.; Golde, D.W.","year":"1978","unstructured":"Gale RP, Sparkes RS, Golde DW. Bone marrow origin of hepatic macrophages (Kupffer cells) in humans. Science 1978;201: 937-8.","journal-title":"Science"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/0008-8749(83)90151-X","article-title":"Differential immunohistochemical resolution of the human mononuclear phagocyte system","volume":"82","author":"Radzun, H.J.; Parwaresch, M.R.","year":"1983","unstructured":"Radzun HJ, Parwaresch MR. Differential immunohistochemical resolution of the human mononuclear phagocyte system. Cell Immunol 1983;82:174-83.","journal-title":"Cell Immunol"},{"key":"ref_6","first-page":"1487","article-title":"A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes","volume":"134","author":"Zwadlo, G.; Brocker, E.B.; von Bassewitz, D.B.; Feige, U.; Sorg, C.","year":"1985","unstructured":"Zwadlo G, Brocker EB, von Bassewitz DB, Feige U, Sorg C. A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes. J Immunol 1985;134:1487-92.","journal-title":"J Immunol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1172\/JCI109076","article-title":"Differentiation of macrophages from normal human bone marrow in liquid culture. Electron microscopy and cytochemistry","volume":"61","author":"Bainton, D.F.; Golde, D.W.","year":"1978","unstructured":"Bainton DF, Golde DW. Differentiation of macrophages from normal human bone marrow in liquid culture. Electron microscopy and cytochemistry. J Clin Invest 1978;61:1555-69.","journal-title":"J Clin Invest"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1182\/blood.V62.6.1271.1271","article-title":"Antigenic heterogeneity of human mononuclear phagocytes: Immunohistologic analysis using mononuclear antibodies","volume":"62","author":"Hancock, W.W.; Zola, H.; Atkins, R.C.","year":"1983","unstructured":"Hancock WW, Zola H, Atkins RC. Antigenic heterogeneity of human mononuclear phagocytes: Immunohistologic analysis using mononuclear antibodies. Blood 1983;62:1271-9.","journal-title":"Blood"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1093\/ajcp\/55.3.283","article-title":"Cytochemical identification of monocytes and granulocytes","volume":"55","author":"Yam, L.T.; Li, C.Y.; Crosby, W.H.","year":"1971","unstructured":"Yam LT, Li CY, Crosby WH. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol 1971;55:283-90.","journal-title":"Am J Clin Pathol"},{"key":"ref_10","first-page":"322","article-title":"Immunohistological analysis of human mononuclear phagocytes and dendritic cells by using monoclonal antibodies","volume":"54","author":"Franklin, W.A.; Mason, D.Y.; Pulford, K.","year":"1986","unstructured":"Franklin WA, Mason DY, Pulford K, et al. Immunohistological analysis of human mononuclear phagocytes and dendritic cells by using monoclonal antibodies. Lab Invest 1986;54:322-35.","journal-title":"Lab Invest"},{"key":"ref_11","first-page":"8","article-title":"Tissue distribution of macrophage cell surface antigens in human spleen","volume":"0","author":"Buckley, P.J.; Dickson, S.A.; Walker, W.S.","year":"1984","unstructured":"Buckley PJ, Dickson SA, Walker WS. Tissue distribution of macrophage cell surface antigens in human spleen. Lab Invest 1984;S0:8A.","journal-title":"Lab Invest"},{"key":"ref_12","first-page":"145","article-title":"Human mononuclear phagocytes differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies","volume":"130","author":"Dimitriu-Bona, A.; Burmester, G.R.; Waters, S.J.; Winchester, R.J.","year":"1983","unstructured":"Dimitriu-Bona A, Burmester GR, Waters SJ, Winchester RJ. Human mononuclear phagocytes differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol 1983;130:145-52.","journal-title":"J Immunol"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/0008-8749(85)90006-1","article-title":"An anti-human monocyte\/macrophage monoclonal antibody, reacting most strongly with macrophages in lymphoid tissue","volume":"92","author":"Hogg, N.; Selvendran, Y.","year":"1985","unstructured":"Hogg N, Selvendran Y. An anti-human monocyte\/macrophage monoclonal antibody, reacting most strongly with macrophages in lymphoid tissue. Cell Immunol 1985;92:247-53.","journal-title":"Cell Immunol"},{"key":"ref_14","first-page":"2170","article-title":"Monoclonal antihuman monocyte antibodies OKM 1 and OKM5 possess distinctive tissue distributions including differential reactivity with vascular endothelium","volume":"132","author":"DM, II, Knowles; B, Tolijain; D, Marboe; V, D.'Agati; M, Grimes; L, Chess","year":"1984","unstructured":"Knowles DM II, Tolijain B, Marboe D, D'Agati V, Grimes M, Chess L. Monoclonal antihuman monocyte antibodies OKM 1 and OKM5 possess distinctive tissue distributions including differential reactivity with vascular endothelium. J Immunol 1984;132:2170-3.","journal-title":"J Immunol"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1084\/jem.152.3.581","article-title":"Macrophage heterogeneity in man. A subpopulation of HLA-DR-bearing macrophages required for antigen-induced T cell activation contains stimulators for autologous-reactive T cells","volume":"152","author":"RaffHV, Picker L.J.; JD, Stobo","year":"1980","unstructured":"RaffHV, Picker LJ, Stobo JD. Macrophage heterogeneity in man. A subpopulation of HLA-DR-bearing macrophages required for antigen-induced T cell activation contains stimulators for autologous-reactive T cells. J Exp Med 1980;152:581-93.","journal-title":"J Exp Med"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1016\/S0140-6736(86)91781-2","article-title":"APAAP labelling of blood and bone marrow samples for phenotyping leukaemia","volume":"i:","author":"Erber, W.N.; Mynheer, L.C.; Mason, D.Y.","year":"1986","unstructured":"Erber WN, Mynheer LC, Mason DY. APAAP labelling of blood and bone marrow samples for phenotyping leukaemia. Lancet 1986;i:761-5.","journal-title":"Lancet"},{"key":"ref_17","article-title":"Production ofmonoclonal antibodies for immunocytochemical use","volume-title":"in immunocytochemistry","author":"Mason, D.Y.; Cordell, J.L.; Pulford, K.A.F.","unstructured":"Mason DY, Cordell JL, Pulford KAF. Production ofmonoclonal antibodies for immunocytochemical use. In: Bullock GR, Petrusz P, eds. Techniques in immunocytochemistry. London: Academic Press, 1983:175."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1177\/32.2.6198355","article-title":"Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes)","volume":"32","author":"Cordell, J.L.; Falini, B.; Erber, W.N.","year":"1984","unstructured":"Cordell JL, Falini B, Erber WN, et al. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 1984;32:219-29.","journal-title":"J Histochem Cytochem"},{"key":"ref_19","first-page":"145","article-title":"Immunoenzymatic labelling ofhaematological samples with monoclonal antibodies","volume-title":"Methods in hematology: monoclonal antibodies","author":"Mason, D.Y.; Erber, W.N.; Falini, B.; Stein, H.; Gatter, K.C.","year":"1986","unstructured":"Mason DY, Erber WN, Falini B, Stein H, Gatter KC. Immunoenzymatic labelling ofhaematological samples with monoclonal antibodies. In: Beverley PCL, ed. Methods in hematology: monoclonal antibodies. Edinburgh: Churchill Livingstone, 1986:145-81."},{"key":"ref_20","first-page":"519","article-title":"The purification of antigens and other studies with monoclonal antibody affinity columns: the complementary new dimension of monoclonal antibodies","volume-title":"Monoclonal antibodies in clinical medicine","author":"Dalchau, R.; Fabre, J.W.","year":"1982","unstructured":"Dalchau R, Fabre JW. The purification of antigens and other studies with monoclonal antibody affinity columns: the complementary new dimension of monoclonal antibodies. In: McMichael AJ, Fabre JW, eds. Monoclonal antibodies in clinical medicine. London: Academic Press, 1982:519-56."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Clearage ofstructural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemli, U.K.","year":"1970","unstructured":"Laemli UK. Clearage ofstructural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-5.","journal-title":"Nature"},{"key":"ref_22","first-page":"4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets","volume-title":"Procedure and some applications. Proc Natil Acad Sci","volume":"76","author":"Towbin, H.; Staehelin, T.; Gordon, J.","year":"1979","unstructured":"Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natil Acad Sci USA 1979;76:4350-4."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1083\/jcb.95.2.580","article-title":"Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies","volume":"95","author":"Woodcock-Mitchell, J.; Eichner, R.; Nelson, W.G.; Sun, T.T.","year":"1982","unstructured":"Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 1982;95:580-8.","journal-title":"J Cell Biol"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1056\/NEJM198003273021301","article-title":"Successful bone marrow transplantation for infantile malignant osteopetrosis","volume":"302","author":"Coccia, P.F.; Krivit, W.; Cervenka, J.","year":"1980","unstructured":"Coccia PF, Krivit W, Cervenka J, et al. Successful bone marrow transplantation for infantile malignant osteopetrosis. N Engl J Med 1980;302:701-8.","journal-title":"N Engl J Med"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1084\/jem.142.3.651","article-title":"Control of bone marrow resorption by hematopoietic tissue. The induction and reversal of congenital osteopetrosis in mice through use ofbone marrow and splenic transplants","volume":"142","author":"Walker, D.G.","year":"1975","unstructured":"Walker DG. Control of bone marrow resorption by hematopoietic tissue. The induction and reversal of congenital osteopetrosis in mice through use ofbone marrow and splenic transplants. J Exp Med 1975;142:651-63.","journal-title":"J Exp Med"},{"key":"ref_26","first-page":"5663","article-title":"Monoclonal antibodies to osteoclastomas (giant cell bone tumors): defiinition of osteoclast-specific cellular antigens","volume":"45","author":"Horton, M.A.; Lewis, D.; McNulty, K.; Pringle, J.A.S.; Chambers, T.J.","year":"1985","unstructured":"Horton MA, Lewis D, McNulty K, Pringle JAS, Chambers TJ. Monoclonal antibodies to osteoclastomas (giant cell bone tumors): defiinition of osteoclast-specific cellular antigens. Cancer Res 1985;45:5663-9.","journal-title":"Cancer Res"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1002\/path.1711500403","article-title":"Osteoclasts contain macrophage and megakaryocyte antigens","volume":"150","author":"Athanasou, N.A.; Heryet, A.; Quinn, J.; Gatter, K.C.; Mason, D.Y.; McGee, J.O.D.","year":"1986","unstructured":"Athanasou NA, Heryet A, Quinn J, Gatter KC, Mason DY, McGee JOD. Osteoclasts contain macrophage and megakaryocyte antigens. J Pathol 1986;150:239-46.","journal-title":"J Pathol"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1002\/eji.1830160306","article-title":"The p150,95 molecule is a marker of human mononuclear phagocytes: Comparison with expression of class II molecules","volume":"16","author":"Hogg, N.; Takas, L.; Palmer, D.G.; Selvendran, Y.; Allan, C.","year":"1986","unstructured":"Hogg N, Takas L, Palmer DG, Selvendran Y, Allan C. The p150,95 molecule is a marker of human mononuclear phagocytes: Comparison with expression of class II molecules. Eur J Immunol 1986;16:240-8.","journal-title":"Eur J Immunol"},{"key":"ref_29","first-page":"427","article-title":"A monoclonal antibody recognizing the p150\/95 leucocyte differentiation antigen","volume":"59","author":"MacDonald, S.M.; Pulford, K.; Falini, B.; Micklem, K.; Mason, D.Y.","year":"1986","unstructured":"MacDonald SM, Pulford K, Falini B, Micklem K, Mason DY. A monoclonal antibody recognizing the p150\/95 leucocyte differentiation antigen. Immunol 1986;59:427-3 1.","journal-title":"Immunol"},{"key":"ref_30","first-page":"141","article-title":"Monocyte\/macrophage-reactive monoclonal antibody Ki-M6 recognizes an intracytoplasmic antigen","volume":"124","author":"Parwaresch, M.R.; Radzun, H.J.; Kreipe, H.; Hansmann, M.L.; Barth, J.","year":"1986","unstructured":"Parwaresch MR, Radzun HJ, Kreipe H, Hansmann ML, Barth J. Monocyte\/macrophage-reactive monoclonal antibody Ki-M6 recognizes an intracytoplasmic antigen. Am J Pathol 1986;124:141-51.","journal-title":"Am J Pathol"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1111\/j.1365-2141.1976.tb03563.x","article-title":"Proposals for the classification of the acute leukaemias","volume":"33","author":"Bennett, J.M.; Catovsky, D.; Daniel, M.T.","year":"1976","unstructured":"Bennett JM, Catovsky D, Daniel MT, et al. Proposals for the classification of the acute leukaemias. Br J Haematol 1976;33:451-8.","journal-title":"Br J Haematol"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1182\/blood.V68.6.1232.1232","article-title":"Use of surface marker analysis to predict outcome of adult acute myeloblastic leukemia","volume":"68","author":"Griffin, J.D.; Davis, R.; Nelson, D.A.","year":"1986","unstructured":"Griffin JD, Davis R, Nelson DA, et al. Use of surface marker analysis to predict outcome of adult acute myeloblastic leukemia. Blood 1986;68:1232-41.","journal-title":"Blood"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1093\/ajcp\/82.1.29","article-title":"Leu Ml and peanut agglutinin stain in the neoplastic cells of Hodgkin's disease","volume":"82","author":"Hsu, S.M.; Jaffe, E.S.","year":"1984","unstructured":"Hsu SM, Jaffe ES. Leu Ml and peanut agglutinin stain in the neoplastic cells of Hodgkin's disease. Am J Clin Pathol 1984;82:29-32.","journal-title":"Am J Clin Pathol"},{"key":"ref_34","first-page":"209","article-title":"Phenotypic expression of Hodgkin's and Reed-Sternberg cells in Hodgkin's disease","volume":"118","author":"Hsu, S.M.; Yang, K.; Jaffe, E.S.","year":"1985","unstructured":"Hsu SM, Yang K, Jaffe ES. Phenotypic expression of Hodgkin's and Reed-Sternberg cells in Hodgkin's disease. Am J Pathol 1985;118:209-17.","journal-title":"Am J Pathol"},{"key":"ref_35","first-page":"370","article-title":"Lectin receptors as markers of lymphoma cells. II. Reed-Stemnberg cells share lectin-binding properties of monocyte macrophages","volume":"116","author":"Strauchen, J.A.","year":"1984","unstructured":"Strauchen JA. Lectin receptors as markers of lymphoma cells. II. Reed-Stemnberg cells share lectin-binding properties of monocyte macrophages. Am J Pathol 1984;116:370-6.","journal-title":"Am J Pathol"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1182\/blood.V66.4.848.bloodjournal664848","article-title":"The expression of Hodgkin's disease associated antigen Ki-l in reactive and neoplastic lymphoid tissue: Evidence that Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells and histiocytic malignancies are derived from activated lymphoid cells","volume":"66","author":"Stein, H.; Mason, D.Y.; Gerdes, J.","year":"1985","unstructured":"Stein H, Mason DY, Gerdes J, et al. The expression of Hodgkin's disease associated antigen Ki-l in reactive and neoplastic lymphoid tissue: Evidence that Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells and histiocytic malignancies are derived from activated lymphoid cells. Blood 1985;66:848-58.","journal-title":"Blood"},{"key":"ref_37","first-page":"293","article-title":"Immunopathology of Hodgkin's disease. Characterization of Reed-Stemnberg cells with monoclonal antibodies","volume":"123","author":"Strauchen, J.A.; Dimitriu-Bona, A.","year":"1986","unstructured":"Strauchen JA, Dimitriu-Bona A. Immunopathology of Hodgkin's disease. Characterization of Reed-Stemnberg cells with monoclonal antibodies. Am J Pathol 1986;123:293-300.","journal-title":"Am J Pathol"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp.41.7.753","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T08:36:18Z","timestamp":1765701378000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp.41.7.753"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,7]]},"references-count":37,"journal-issue":{"issue":"7","published-online":{"date-parts":[[1988,7,1]]},"published-print":{"date-parts":[[1988,7]]}},"alternative-id":["10.1136\/jcp.41.7.753"],"URL":"https:\/\/doi.org\/10.1136\/jcp.41.7.753","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,7]]}}}